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1. 发明申请

WO2007041201A2 COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS 审中-公开
标题翻译：用于放大核酸的组合物，方法和试剂盒
公开(公告)号：WO2007041201A2
公开(公告)日：2007-04-12
申请号：PCT/US2006/037829
申请日：2006-09-29
申请人： APPLERA CORPORATION , DONG, Shoulian , STEVENS, Junko , LEE, Danny H.
发明人： DONG, Shoulian , STEVENS, Junko , LEE, Danny H.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6846 , C12N15/115 , C12N2310/16 , C12P19/34 , C12Q1/6848 , G01N21/6486 , C12Q2549/125 , C12Q2527/127 , C12Q2527/107
摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.
摘要翻译：本教导涉及用于扩增靶核酸的组合物，方法和试剂盒，同时减少非特异性荧光和不期望的扩增产物，有时称为二级扩增产物或假副产物。本文公开的酶抑制剂包含核苷酸序列和至少一种猝灭剂。还提供了包含与酶相关的酶抑制剂的复合物，其中所述酶的至少一种酶活性被抑制。公开了减少不期望的扩增产物同时扩增靶核酸的方法，还有用于降低非特异性荧光的方法。还提供了用于加速某些公开方法的执行的套件。

2. 发明申请

WO2012159025A2 CHROMOSOME CONFORMATION ANALYSIS 审中-公开
标题翻译：染色体构象分析
公开(公告)号：WO2012159025A2
公开(公告)日：2012-11-22
申请号：PCT/US2012/038558
申请日：2012-05-18
申请人： LIFE TECHNOLOGIES CORPORATION , DONG, Shoulian , STEVENS, Junko F. , LIU, Chunmei , WOO, Cora L. , MONTESCLAROS, Luz
发明人： DONG, Shoulian , STEVENS, Junko F. , LIU, Chunmei , WOO, Cora L. , MONTESCLAROS, Luz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6827 , C12Q1/6848 , C12Q1/6886 , C12Q2600/154 , C12Q2600/158 , C12Q2600/178 , C12Q2523/101 , C12Q2537/164 , C12Q2565/133 , C12Q2545/113 , C12Q2525/173 , C12Q2525/191
摘要： Disclosed herein are compositions, methods and kits for analyzing three-dimensional chromatin and/or chromosome conformation. Method are also disclosed for using the methods disclosed herein for diagnosing diseases such as cancer.
摘要翻译：本文公开了用于分析三维染色质和/或染色体构象的组合物，方法和试剂盒。还公开了使用本文公开的用于诊断疾病如癌症的方法的方法。

3. 发明申请

WO2010022098A3 DNA METHYLATION DETECTION METHODS 审中-公开
公开(公告)号：WO2010022098A3
公开(公告)日：2010-02-25
申请号：PCT/US2009/054220
申请日：2009-08-18
申请人： LIFE TECHNOLOGIES CORPORATION , DONG, Shoulian , STEVENS, Junko
发明人： DONG, Shoulian , STEVENS, Junko
IPC分类号： C12Q1/68 , C12N15/11
摘要： The present teachings provide DNA methylation quantification methods that avoid bisulfite treatment of DNA. Methylation-specific binding proteins (MeDNA binding proteins) and non-methylation specific binding proteins (non-MeDNA binding proteins) are employed in various embodiments to modulate the accessibility of nucleic acids to primer extension reactions. After selectively removing the target nucleic acids, the extension products can be analyzed and methylation quantitated. In some embodiments, the analysis comprises real-time PCR.

4. 发明申请

WO2003012118A1 COMPLEXITY MANAGEMENT OF GENOMIC DNA 审中-公开
标题翻译：基因组DNA的复杂管理
公开(公告)号：WO2003012118A1
公开(公告)日：2003-02-13
申请号：PCT/US2002/023568
申请日：2002-07-23
申请人： AFFYMETRIX, INC. , DONG, Shoulian
发明人： DONG, Shoulian
IPC分类号： C12P19/34
CPC分类号： C12Q1/6855 , C12N15/1093 , C12Q1/6806 , C12Q1/683 , C12Q1/686 , Y10T436/143333 , C12Q2521/301
摘要： The presently claimed invention provides for novel methods and kits for reducing the complexity of a nucleic acid sample by providing non-gel based methods for amplification of a subset of the sequences in a sample. In a preferred embodiment, amplification of a subset can be accomplished by digesting a sample with two or more restriction enzymes and ligating adaptors to the fragments so that only a subset of the fragments can be amplified. The invention further provides for analysis of the above amplified sample by hybridization to an array, which may be specifically designed to interrogate the desired fragments for particular characteristics, such as, for example, the presence or absence of a polymorphism.
摘要翻译：目前要求保护的发明提供了用于通过提供用于扩增样品中的序列的子集的非凝胶的方法来降低核酸样品的复杂性的新方法和试剂盒。在一个优选的实施方案中，子集的扩增可以通过用两个或多个限制性内切酶消化样品并且将连接适配子连接到片段来实现，从而只能扩增片段的一个子集。本发明进一步提供了通过与阵列杂交来分析上述扩增的样品，其可以被特别设计成询问特定特征的期望片段，例如多态性的存在或不存在。

5. 发明公开

EP1943348A2 COMPOSITIONS, METHODS, AND KITS FOR AMPLIFYING NUCLEIC ACIDS 有权转让
标题翻译：用于扩增核酸组合物，方法和试剂盒
公开(公告)号：EP1943348A2
公开(公告)日：2008-07-16
申请号：EP06815660.3
申请日：2006-09-29
申请人： Applera Corporation
发明人： DONG, Shoulian , STEVENS, Junko , LEE, Danny H.
IPC分类号： C12P19/34
CPC分类号： C12Q1/6846 , C12N15/115 , C12N2310/16 , C12P19/34 , C12Q1/6848 , G01N21/6486 , C12Q2549/125 , C12Q2527/127 , C12Q2527/107
摘要： The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided.

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