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1. 发明申请

WO2003046146A2 COMPOSITIONS AND METHODS OF SELECTIVE NUCLEIC ACID ISOLATION 审中-公开
标题翻译：选择性核酸分离的组合物和方法
公开(公告)号：WO2003046146A2
公开(公告)日：2003-06-05
申请号：PCT/US2002/038123
申请日：2002-11-27
申请人： APPLERA CORPORATION , BOST, Douglas, A. , GREENFIELD, Lawrence, I
发明人： BOST, Douglas, A. , GREENFIELD, Lawrence, I
IPC分类号： C12N
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
摘要翻译：本发明涉及用于分离和/或鉴定核酸的方法。本发明还提供用于分离和/或鉴定核酸的试剂盒。

2. 发明申请

WO2005066359A1 MULTIPLEX PROTEASE PROFILING 审中-公开
标题翻译： MULTIPLEX PROTEASE配置文件
公开(公告)号：WO2005066359A1
公开(公告)日：2005-07-21
申请号：PCT/US2004/043438
申请日：2004-12-23
申请人： APPLERA CORPORATION , SUN, Hongye , GREENFIELD, Lawrence, I. , BOST, Douglas, A.
发明人： SUN, Hongye , GREENFIELD, Lawrence, I. , BOST, Douglas, A.
IPC分类号： C12Q1/37
CPC分类号： C12Q1/37
摘要： In certain embodiments, provided are methods and compositions useful for the detection and quantitation of catalytically active enzymes, particularly catalytically active proteases. The compositions comprise one or more multifunctional tags, wherein each different multifunctional tag comprises a different mobility modifier, a partitioner, a reporter, and a peptide substrate that is specifically or substantially specifically hydrolyzed by a target protease or target protease family. Hydrolysis of each different multifunctional tag by a target protease provides a different labeled hydrolytic product comprising a reporter and a mobility modifier but not a partitioner, where the mobility modifier confers a distinctive mobility on the labeled hydrolytic product in a mobility-dependent analysis technique.
摘要翻译：在某些实施方案中，提供了可用于检测和定量催化活性酶，特别是催化活性蛋白酶的方法和组合物。组合物包含一个或多个多功能标签，其中每个不同的多功能标签包含由靶蛋白酶或靶蛋白酶家族特异性或基本上特异性水解的不同的迁移率调节剂，分隔器，报道分子和肽底物。通过靶蛋白酶水解每种不同的多功能标签物提供了不同的标记的水解产物，其包含报道分子和迁移率调节剂，而不是分配器，其中迁移率调节剂在迁移依赖性分析技术中赋予标记的水解产物上独特的迁移率。

3. 发明申请

WO2003046146A3 COMPOSITIONS AND METHODS OF SELECTIVE NUCLEIC ACID ISOLATION 审中-公开
公开(公告)号：WO2003046146A3
公开(公告)日：2003-06-05
申请号：PCT/US2002/038123
申请日：2002-11-27
申请人： APPLERA CORPORATION , BOST, Douglas, A. , GREENFIELD, Lawrence, I
发明人： BOST, Douglas, A. , GREENFIELD, Lawrence, I
IPC分类号： C12Q1/68
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.

4. 发明申请

WO2005056836A2 METHOD FOR GENERATING A PREDETERMINED AMOUNT OF NUCLEIC ACID 审中-公开
标题翻译：产生预定量的核酸的方法
公开(公告)号：WO2005056836A2
公开(公告)日：2005-06-23
申请号：PCT/US2004/017928
申请日：2003-12-18
申请人： APPLERA CORPORATION , GREENFIELD, I., Lawrence , BOST, Douglas, A.
发明人： GREENFIELD, I., Lawrence , BOST, Douglas, A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q2527/125 , C12Q2527/119
摘要： A method is provided for binding a predetermined amount of a nucleic acid. The method involves contacting one or more sample solutions comprising a nucleic acid with a multiplicity of solid substrate binding units, where the solid substrate binding units bind the nucleic acid. Each binding unit has a predetermined binding capacity for the nucleic acid. An apparatus for binding one or more predetermined amounts of a nucleic acid is also provided.
摘要翻译：提供了用于结合预定量的核酸的方法。该方法包括使包含核酸的一种或多种样品溶液与多种固体底物结合单元接触，其中固体底物结合单元与核酸结合。每个结合单元对核酸具有预定的结合能力。还提供了用于结合一个或多个预定量的核酸的装置。

5. 发明申请

WO2004009851A2 METHOD FOR PCR CLEANUP AND OLIGONUCLEOTIDE REMOVAL 审中-公开
标题翻译： PCR清除和寡核苷酸去除方法
公开(公告)号：WO2004009851A2
公开(公告)日：2004-01-29
申请号：PCT/US2003/023022
申请日：2003-07-20
申请人： APPLERA CORPORATION , GREENFIELD, Lawrence , BOST, Douglas, A.
发明人： GREENFIELD, Lawrence , BOST, Douglas, A.
IPC分类号： C12S
CPC分类号： C12Q1/6848 , C12Q2525/197
摘要： A method is provided for purifying a desired polynucleotide product by removing unincorporated oligonucleotides from a polymerase or ligase reaction mixture.
摘要翻译：提供了通过从聚合酶或连接酶反应混合物中除去未掺入的寡核苷酸来纯化所需多核苷酸产物的方法。

6. 发明公开

EP1579015A3 METHOD FOR PCR CLEANUP AND OLIGONUCLEOTIDE REMOVAL 审中-公开
标题翻译：用于PCR清除和寡核苷酸去除的方法
公开(公告)号：EP1579015A3
公开(公告)日：2005-10-12
申请号：EP03765969.5
申请日：2003-07-20
申请人： Applera Corporation
发明人： GREENFIELD, Lawrence , BOST, Douglas, A.
IPC分类号： C12S1/00

7. 发明公开

EP1579015A2 METHOD FOR PCR CLEANUP AND OLIGONUCLEOTIDE REMOVAL 审中-公开
标题翻译：用于PCR清除和寡核苷酸去除的方法
公开(公告)号：EP1579015A2
公开(公告)日：2005-09-28
申请号：EP03765969.5
申请日：2003-07-20
申请人： Applera Corporation
发明人： GREENFIELD, Lawrence , BOST, Douglas, A.
IPC分类号： C12S1/00
CPC分类号： C12Q1/6848 , C12Q2525/197
摘要： A method is provided for purifying a desired polynucleotide product by removing unincorporated oligonucleotides from a polymerase or ligase reaction mixture.
摘要翻译：提供了通过从聚合酶或连接酶反应混合物中除去未掺入的寡核苷酸来纯化所需多核苷酸产物的方法。

8. 发明公开

EP1704245A1 MULTIPLEX PROTEASE PROFILING 审中-公开
标题翻译：多重PROTEASEPROFILERSTELLUNG
公开(公告)号：EP1704245A1
公开(公告)日：2006-09-27
申请号：EP04815506.3
申请日：2004-12-23
申请人： Applera Corporation
发明人： SUN, Hongye , GREENFIELD, Lawrence, I. , BOST, Douglas, A.
IPC分类号： C12Q1/37
CPC分类号： C12Q1/37
摘要： In certain embodiments, provided are methods and compositions useful for the detection and quantitation of catalytically active enzymes, particularly catalytically active proteases. The compositions comprise one or more multifunctional tags, wherein each different multifunctional tag comprises a different mobility modifier, a partitioner, a reporter, and a peptide substrate that is specifically or substantially specifically hydrolyzed by a target protease or target protease family. Hydrolysis of each different multifunctional tag by a target protease provides a different labeled hydrolytic product comprising a reporter and a mobility modifier but not a partitioner, where the mobility modifier confers a distinctive mobility on the labeled hydrolytic product in a mobility-dependent analysis technique.

9. 发明公开

EP1448799A2 COMPOSITIONS AND METHODS OF SELECTIVE NUCLEIC ACID ISOLATION 有权
标题翻译：组合物和方法用于核酸的选择性分离
公开(公告)号：EP1448799A2
公开(公告)日：2004-08-25
申请号：EP02794065.9
申请日：2002-11-27
申请人： Applera Corporation
发明人： BOST, Douglas, A. , GREENFIELD, Lawrence, I
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.

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