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1. 发明申请

WO2005098040A8 LIGATION AND AMPLIFICATION REACTIONS FOR DETERMINING TARGET MOLECULES 审中-公开
标题翻译：用于确定目标分子的放射反应和放大反应
公开(公告)号：WO2005098040A8
公开(公告)日：2005-12-01
申请号：PCT/US2005010184
申请日：2005-03-24
申请人： APPLERA CORP , ANDERSEN MARK R , LIVAK KENNETH J , BROOMER ADAM , CHEN CAIFU
发明人： ANDERSEN MARK R , LIVAK KENNETH J , BROOMER ADAM , CHEN CAIFU
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6851 , C12Q2525/191 , C12Q2525/185 , C12Q2521/501 , C12Q2525/155 , C12Q2521/313 , C12Q2537/143 , C12Q2525/161
摘要： The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences and proteins in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample for example, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one labeling probe and at least one address primer can be employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments, the encoding comprises a ligation reaction with linker probes, and single nucleotide polymorphisms (SNPs) are analyzed.
摘要翻译：本发明涉及用于使用编码和解码反应检测至少一个样品中靶多核苷酸序列和蛋白质的存在或不存在（或定量）的方法，试剂和试剂盒。当特定靶多核苷酸例如存在于样品中时，在包含可寻址引物部分的编码反应中形成反应产物。可以在解码扩增反应中使用至少一个标记探针和至少一个地址引物，从而根据序列是否存在提供可检测的信号值。在一些实施方案中，编码包含与接头探针的连接反应，并分析单核苷酸多态性（SNP）。

2. 发明申请

WO2005098040A3 LIGATION AND AMPLIFICATION REACTIONS FOR DETERMINING TARGET MOLECULES 审中-公开
标题翻译：用于确定目标分子的配位和放大反应
公开(公告)号：WO2005098040A3
公开(公告)日：2006-03-23
申请号：PCT/US2005010184
申请日：2005-03-24
申请人： APPLERA CORP , ANDERSEN MARK R , LIVAK KENNETH J , BROOMER ADAM , CHEN CAIFU
发明人： ANDERSEN MARK R , LIVAK KENNETH J , BROOMER ADAM , CHEN CAIFU
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/6851 , C12Q2525/191 , C12Q2525/185 , C12Q2521/501 , C12Q2525/155 , C12Q2521/313 , C12Q2537/143 , C12Q2525/161
摘要： The present invention is directed to methods, reagents, and kits for detecting the presence or absence of (or quantifying) target polynucleotide sequences and proteins in at least one sample using encoding and decoding reactions. When a particular target polynucleotide is present in a sample for example, a reaction product is formed in the encoding reaction that includes addressable primer portions. At least one labeling probe and at least one address primer can be employed in the decoding amplification reaction thereby providing a detectable signal value depending upon whether a sequence is present or absent. In some embodiments, the encoding comprises a ligation reaction with linker probes, and single nucleotide polymorphisms (SNPs) are analyzed.

3. 发明申请

WO2006071568A3 METHODS, COMPOSITIONS, AND KITS FOR FORMING LABELED POLYNUCLEOTIDES 审中-公开
标题翻译：用于形成标记多核苷酸的方法，组合物和试剂盒
公开(公告)号：WO2006071568A3
公开(公告)日：2006-10-05
申请号：PCT/US2005045615
申请日：2005-12-15
申请人： APPLERA CORP , HENNESSY LORI K , ANDERSEN MARK R
发明人： HENNESSY LORI K , ANDERSEN MARK R
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6806 , C12Q2565/102 , C12Q2537/162 , C12Q2521/501
摘要： The present teachings provide novel methods, compositions, and kits for forming multi-labeled polynucleotides. In some embodiments of the present teachings, multiplexed amplification reactions are performed with a plurality of primer pairs, wherein one primer in a given primer pair comprises a distinct label. Additional labeling of the resulting amplicons can be accomplished by using at least one bridge oligonucleotide to ligate a labeled tag oligonucleotide to each labeled extension product, thereby forming a plurality of multi-labeled polynucleotides. Detection of labels such as florophores and mobility modifiers in the plurality of multi-labeled polynucleotides can identify a sample. Such sample identification can be performed using a mobility dependent analysis technique such as capillary electrophoresis, and can applicable in the field of forensics.
摘要翻译：本教导提供了用于形成多标记多核苷酸的新方法，组合物和试剂盒。在本教导的一些实施方案中，用多个引物对进行多重扩增反应，其中给定引物对中的一个引物包含不同的标记。通过使用至少一个桥寡核苷酸将标记的标签寡核苷酸连接到每个标记的延伸产物上，由此形成多个多标记的多核苷酸，可以实现所得扩增子的额外标记。检测多种多标记多核苷酸中的标记物例如荧光团和迁移率调节剂可以鉴定样品。这种样品鉴定可以使用诸如毛细管电泳之类的与移动性相关的分析技术来进行，并且可以应用于取证领域。

4. 发明申请

WO2006028496A3 LESION REPAIR POLYMERASE COMPOSITIONS 审中-公开
标题翻译： LESION维修聚合物组合物
公开(公告)号：WO2006028496A3
公开(公告)日：2006-12-07
申请号：PCT/US2005005242
申请日：2005-02-18
申请人： APPLERA CORP , ANDERSEN MARK R
发明人： ANDERSEN MARK R
IPC分类号： C12Q1/68 , A61K38/47
CPC分类号： C12Q1/686 , C12N9/1252 , C12Q2527/149 , C12Q2521/101
摘要： Compositions comprising at least two polymerases, including a lesion repair polymerase, are provided. Methods for producing primer extension products using at least two polymerases, including a lesion repair polymerase, are also provided. Kits for producing primer extension products comprising at least two polymerases, including a lesion repair polymerase, are also provided.
摘要翻译：提供包含至少两种聚合酶（包括病变修复聚合酶）的组合物。还提供了使用至少两种聚合酶（包括损伤修复聚合酶）产生引物延伸产物的方法。还提供了用于生产包含至少两种聚合酶（包括病变修复聚合酶）的引物延伸产物的试剂盒。

5. 发明申请

WO2004051218A3 MULTIPLEX AMPLIFICATION OF POLYNUCLEOTIDES 审中-公开
标题翻译：多核苷酸的多重扩增
公开(公告)号：WO2004051218A3
公开(公告)日：2005-02-17
申请号：PCT/US0337808
申请日：2003-11-26
申请人： APPLERA CORP , ANDERSEN MARK R , RUFF DAVID W
发明人： ANDERSEN MARK R , RUFF DAVID W
IPC分类号： C07H21/02 , C07H21/04 , C12P19/34 , C12Q1/68 , G01N20060101
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6827 , C12Q1/6851 , C12Q2531/113 , C12Q2537/143 , C12Q2561/113 , C12Q2521/107 , C12Q2525/301 , C12Q2561/101
摘要： The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis.
摘要翻译：本发明提供用于进行适于分析多核苷酸或样品的多种测定法的方法，试剂和试剂盒，所述测定包括以多种方式进行的扩增步骤。还提供了用于分析和提高扩增效率和进行基因表达分析的方法。

6. 发明申请

WO2004051218A8 MULTIPLEX AMPLIFICATION OF POLYNUCLEOTIDES 审中-公开
标题翻译：多核苷酸的多重扩增
公开(公告)号：WO2004051218A8
公开(公告)日：2004-09-10
申请号：PCT/US0337808
申请日：2003-11-26
申请人： APPLERA CORP , ANDERSEN MARK R , RUFF DAVID W
发明人： ANDERSEN MARK R , RUFF DAVID W
IPC分类号： C07H21/02 , C07H21/04 , C12P19/34 , C12Q1/68 , G01N20060101 , G01N
CPC分类号： C12Q1/686 , C12Q1/6806 , C12Q1/6827 , C12Q1/6851 , C12Q2531/113 , C12Q2537/143 , C12Q2561/113 , C12Q2521/107 , C12Q2525/301 , C12Q2561/101
摘要： The present invention provides methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples that include an amplification step performed in a multiplex fashion. Also provided are methods for analyzing and improving the efficiency of amplification and for carrying out gene expression analysis.
摘要翻译：本发明提供了用于进行适合分析包括以多重方式进行的扩增步骤的多核苷酸或样品的各种分析的方法，试剂和试剂盒。还提供了分析和提高扩增效率和进行基因表达分析的方法。

7. 发明专利

CA2499360A1 METHODS AND COMPOSITIONS FOR DETECTING TARGETS 未知
公开(公告)号：CA2499360A1
公开(公告)日：2004-04-01
申请号：CA2499360
申请日：2003-09-19
申请人： APPLERA CORP
发明人： ANDERSEN MARK R , WENZ MICHAEL H , SPIER EUGENE G , LIVAK KENNETH J , HUNKAPILLER MICHAEL W
IPC分类号： C07H21/00 , C07H21/02 , C07H21/04 , C12P19/34 , C12Q20060101 , C12Q1/68
摘要： The present invention relates to methods and kits for detecting the presence or absence of (or quantitating) target nucleic acid sequences using ligation and amplification. The invention also relates to methods, reagents, and kits that employ addressable portions and labeled probes.

8. 发明公开

EP1532275A4 MG-MEDIATED HOT START BIOCHEMICAL REACTIONS 审中-公开
标题翻译：生化MG介导WARMSTART反应的
公开(公告)号：EP1532275A4
公开(公告)日：2005-09-14
申请号：EP03771719
申请日：2003-07-23
申请人： APPLERA CORP
发明人： ANDERSEN MARK R
IPC分类号： B01F5/00 , B01F11/00 , B01F11/02 , B01F13/00 , B01F15/02 , B01F15/06 , B01L3/00 , B01L7/00 , C12M1/34 , C12Q1/68 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： B01F15/0213 , B01F5/0057 , B01F11/0005 , B01F11/0082 , B01F11/02 , B01F11/0266 , B01F13/0059 , B01F15/0205 , B01F15/0215 , B01F2005/0017 , B01F2015/062 , B01L3/502738 , B01L7/52 , B01L2300/0803 , B01L2300/0806 , B01L2300/0816 , B01L2300/087 , B01L2400/0409 , B01L2400/0487 , B01L2400/0683 , C12Q1/686 , C12Q2565/629
摘要： Microfluidic devices are provided that are adapted to retain reactants in first and second chambers that can be in openable fluid communication with each other. The reactants can be reactants necessary to initiate, promote, or catalyze a polymerase chain reaction another nucleic acid sequence amplification, detection, ligation, or endonuclease reaction or a nucleic acid sequencing reaction. Methods and systems are also provided.

9. 发明专利

AU2003298706A1 MULTIPLEX AMPLIFICATION OF POLYNUCLEOTIDES 未知
公开(公告)号：AU2003298706A1
公开(公告)日：2004-06-23
申请号：AU2003298706
申请日：2003-11-26
申请人： APPLERA CORP
发明人： ANDERSEN MARK R , RUFF DAVID W
IPC分类号： C07H21/02 , C07H21/04 , C12P19/34 , G01N20060101

10. 发明专利

AU2003256675A8 Mg-mediated hot start biochemical reactions 未知
公开(公告)号：AU2003256675A8
公开(公告)日：2004-02-16
申请号：AU2003256675
申请日：2003-07-23
申请人： APPLERA CORP
发明人： ANDERSEN MARK R
IPC分类号： B01F5/00 , B01F11/00 , B01F11/02 , B01F13/00 , B01F15/02 , B01F15/06 , B01L3/00 , B01L7/00 , C12M1/34 , C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
摘要： Microfluidic devices are provided that are adapted to retain reactants in first and second chambers that can be in openable fluid communication with each other. The reactants can be reactants necessary to initiate, promote, or catalyze a polymerase chain reaction another nucleic acid sequence amplification, detection, ligation, or endonuclease reaction or a nucleic acid sequencing reaction. Methods and systems are also provided.

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