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1. 发明申请

WO0203917A3 ALPHAVIRUS VECTORS AND VIROSOMES WITH MODIFIED HIV GENES FOR USE AS VACCINES 审中-公开
标题翻译：具有修饰的HIV基因的野生型病毒和病毒用作疫苗
公开(公告)号：WO0203917A3
公开(公告)日：2002-08-29
申请号：PCT/US0121701
申请日：2001-07-09
申请人： ALPHAVAX INC , UNIV NORTH CAROLINA , OLMSTED ROBERT , KEITH PAULA , DRYGA SERGEY , CALEY IAN , MAUGHAN MAUREEN , JOHNSTON ROBERT , DAVIS NANCY , SWANSTROM RONALD
发明人： OLMSTED ROBERT , KEITH PAULA , DRYGA SERGEY , CALEY IAN , MAUGHAN MAUREEN , JOHNSTON ROBERT , DAVIS NANCY , SWANSTROM RONALD
IPC分类号： A61K39/00 , A61K39/21 , C07K14/16 , C07K14/18 , C12N7/02 , A61K39/12
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/5256 , A61K2039/54 , A61K2039/545 , A61K2039/57 , C12N7/00 , C12N2740/16034 , C12N2740/16122 , C12N2740/16134 , C12N2740/16222 , C12N2740/16234 , C12N2770/36143 , C12N2770/36151
摘要： The present invention provides methods and compositions comprising a population of alphavirus replicon particles comprising two or more isolated nucleic acids selected from 1) an isolated nucleic acid encoding an env gene product or an immunogenic fragment thereof of a human immunodeficiency virus, 2) an isolated nucleic acid encoding a gag gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein thegag gene product or immunogenic fragment thereof is modified to inhibit formation of virus-like particles containing thegag gene product or the immunogenic fragment thereof and their release from a cell, and 3) an isolated nucleic acid encoding a pol gene product or an immunogenic fragment thereof of a human immunodeficiency virus, wherein the pol gene product or immunogenic fragment thereof is modified to inhibit integrase, Rnase H and/or reverse transcriptase activity, and wherein the nucleic acids are each contained within a separate alphavirus replicon particle.
摘要翻译：本发明提供方法和组合物，其包含包含两种或更多种分离的核酸的甲型病毒复制子颗粒群，所述分离的核酸选自1）编码人免疫缺陷病毒的env基因产物或其免疫原性片段的分离的核酸，2）分离的核酸编码人免疫缺陷病毒的gag基因产物或其免疫原性片段的酸，其中修饰所述gag基因产物或其免疫原性片段以抑制含有所述gag基因产物或其免疫原性片段的病毒样颗粒及其从细胞释放的病毒样颗粒的形成和3）编码人免疫缺陷病毒的pol基因产物或其免疫原性片段的分离的核酸，其中pol基因产物或其免疫原性片段被修饰以抑制整合酶，Rnase H和/或逆转录酶活性，并且其中核酸各自包含在单独的甲病毒复制子中粒子。

2. 发明专利

AU7331301A Alphavirus vectors and virosomes with modified hiv genes for use as vaccines 未知
公开(公告)号：AU7331301A
公开(公告)日：2002-01-21
申请号：AU7331301
申请日：2001-07-09
申请人： ALPHAVAX INC , UNIV NORTH CAROLINA
发明人： OLMSTED ROBERT , KEITH PAULA , DRYGA SERGEY , CALEY IAN , MAUGHAN MAUREEN , JOHNSTON ROBERT , DAVIS NANCY , SWANSTROM RONALD
IPC分类号： A61K39/00 , A61K39/21 , C07K14/16 , C07K14/18 , C12N7/02
摘要： The invention provides a process for the selection of HIV-1 subtype (clade) C isolates, selected HIV-1 subtype C isolates, their genes and modifications and derivatives thereof for use in prophylactic and therapeutic vaccines to produce proteins and polypeptides for the purpose of eliciting protection against HIV infection or disease. The process for the selection of HIV subtype isolates comprises the steps of isolating viruses from recently infected subjects; generating a consensus sequence for at least part of at least one HIV gene by identifying the most common codon or amino acid among the isolated viruses; and selecting the isolated virus or viruses with a high sequence identity to the consensus sequence. HIV-1 subtype C isolates, designated Du422, Du 151 and Du 179 (assigned Accession Numbers 01032114, 00072724 and 00072725, respectively, by the European Collection of Cell Cultures) are also provided.

3. 发明申请

WO2004055166A3 MULTI-ANTIGENIC ALPHAVIRUS REPLICON PARTICLES AND METHODS 审中-公开
标题翻译：多重抗原性阿尔法病毒复制粒子和方法
公开(公告)号：WO2004055166A3
公开(公告)日：2004-09-23
申请号：PCT/US0339723
申请日：2003-12-12
申请人： ALPHAVAX INC
发明人： SMITH JONATHAN F , KAMRUD KURT , DRYGA SERGEY , CALEY IAN
IPC分类号： A61K48/00 , C12N7/04 , C12N15/86 , C12N7/00 , A01N63/00 , C07H21/02 , C12N5/06 , C12N5/10 , C12N15/63
CPC分类号： C12N7/00 , A61K48/00 , A61K2039/5256 , A61K2039/70 , C12N15/86 , C12N2740/16222 , C12N2770/36134 , C12N2770/36143 , C12N2770/36152
摘要： Viral replicon selected nucleic acid expression libraries are useful for analyzing multiple antigens associated with a parasite, pathogen or neoplasia or for preparing immunogenic compositions for generating immune responses specific for the parasite pathogen or neoplasia. Alphavirus replicon particles representative of the nucleic acid expression library are preferred. The nucleic acid library can be a random library, or it can be prepared after a selection step, for example, by differential hybridization prior to cloning into the replicon vector.
摘要翻译：病毒复制子选择的核酸表达文库可用于分析与寄生虫，病原体或瘤形成有关的多种抗原或用于制备用于产生对寄生虫病原体或瘤形成特异性的免疫应答的免疫原性组合物。代表核酸表达文库的甲病毒属复制子颗粒是优选的。核酸文库可以是随机文库，或者可以在选择步骤之后制备，例如在克隆入复制子载体之前通过差异杂交。

4. 发明专利

DK1585812T3 MULTI-ANTIGENE ALFAVIRUSREPLIKONPARTIKLER OG FREMGANGSMÅDER 未知
公开(公告)号：DK1585812T3
公开(公告)日：2017-04-10
申请号：DK03813432
申请日：2003-12-12
申请人： ALPHAVAX INC
发明人： SMITH JONATHAN F , KAMRUD KURT , DRYGA SERGEY , CALEY IAN
IPC分类号： C12N7/00 , A01N63/00 , A61K39/00 , A61K48/00 , C07H21/02 , C12N5/07 , C12N5/10 , C12N7/04 , C12N15/63 , C12N15/86

5. 发明专利

CA2509973C MULTI-ANTIGENIC ALPHAVIRUS REPLICON PARTICLES AND METHODS 未知
公开(公告)号：CA2509973C
公开(公告)日：2013-02-26
申请号：CA2509973
申请日：2003-12-12
申请人： ALPHAVAX INC
发明人： SMITH JONATHAN F , KAMRUD KURT , DRYGA SERGEY , CALEY IAN
IPC分类号： C12N7/00 , A61K39/00 , A61K48/00 , C12N7/01 , C12N7/04 , C12N15/86 , C40B40/02 , C40B50/06
摘要： Viral replicon selected nucleic acid expression libraries are useful for analyzing multiple antigens associated with a parasite, pathogen or neoplasia or for preparing immunogenic compositions for generating immune responses specific for the parasite pathogen or neoplasia. Alphavirus replicon particles representative of the nucleic acid expression library are preferred. The nucleic acid library can be a random library, or it can be prepared after a selection step, for example, by differential hybridization prior to cloning into the replicon vector.

6. 发明专利

AU2003297039B2 Multi-antigenic alphavirus replicon particles and methods 未知
公开(公告)号：AU2003297039B2
公开(公告)日：2009-03-05
申请号：AU2003297039
申请日：2003-12-12
申请人： ALPHAVAX INC
发明人： CALEY IAN , SMITH JONATHAN F , KAMRUD KURT , DRYGA SERGEY
IPC分类号： C12N7/04 , A61K48/00 , C12N15/86
摘要： Viral replicon selected nucleic acid expression libraries are useful for analyzing multiple antigens associated with a parasite, pathogen or neoplasia or for preparing immunogenic compositions for generating immune responses specific for the parasite, pathogen or neoplasia. Alphavirus replicon particles representative of the nucleic acid expression library are preferred. The nucleic acid library can be a random library, or it can be prepared after a selection step, for example, by differential hybridization prior to cloning into the replicon vector.

7. 发明专利

ES2618309T3 Partículas de replicón de alfavirus multi-antigénico y métodos 未知
公开(公告)号：ES2618309T3
公开(公告)日：2017-06-21
申请号：ES03813432
申请日：2003-12-12
申请人： ALPHAVAX INC
发明人： SMITH JONATHAN , KAMRUD KURT , DRYGA SERGEY , CALEY IAN
IPC分类号： C12N7/00 , A01N63/00 , A61K39/00 , A61K48/00 , C07H21/02 , C12N5/07 , C12N5/10 , C12N7/04 , C12N15/63 , C12N15/86
摘要： Un método para preparar una población de partículas de replicón de virus (VRPs) del virus de encefalitis equina venezolana (VEE) que colectivamente codifican y expresan una biblioteca de expresión de un patógeno, parásito o célula tumoral, en donde las partículas con defecto de propagación no pueden producir partículas de progenie debido a la ausencia en el ARN del replicón de al menos una secuencia que codifica para una proteína estructural del virus VEE requerida para el empaquetamiento del ácido nucleico del replicón, comprendiendo dicho método las etapas de: a) introducir una pluralidad de ácidos nucleicos de replicón de VEE en una pluralidad de células, en donde dichas células son permisivas para la replicación y empaquetamiento de VEE, en donde dicho ácido nucleico de replicón de VEE comprende al menos una señal de empaquetamiento de virus VEE y al menos una secuencia codificadora heteróloga expresable a partir de dicho ácido nucleico de replicón de VEE, en donde las secuencias que codifican las proteínas estructurales requeridas para el empaquetamiento del ácido nucleico de replicón se distribuyen entre uno o más ácidos nucleicos colaboradores y el ARN de replicón, y en donde el uno o más ácidos nucleicos colaboradores se seleccionan de entre al menos dos moléculas de ARN colaborador o una molécula de ADN, siempre que se elimine al menos una secuencia codificadora de proteína estructural del ARN de replicón, de tal modo que la partícula de VEE resultante tenga defecto de propagación, y en donde la pluralidad de ácidos nucleicos de replicón de VEE codifican la biblioteca de expresión de un patógeno, parásito o célula tumoral, para producir una pluralidad de células modificadas; b) cultivar dicha pluralidad de células modificadas de la etapa (a) en condiciones que permitan la expresión del uno o más ácidos nucleicos colaboradores, permitiendo la replicación de dicho ácido nucleico de replicón de VEE y el empaquetamiento de dicho ácido nucleico de replicón de VEE para formar VRPs; c) poner en contacto las células modificadas tras la etapa (b) con un medio acuoso que contiene entre 0,2 M y 5 M de una sal para liberar las VRPs al medio acuoso para producir una disolución que contiene VRPs; y d) recolectar las VRPs de la disolución que contiene VRPs de la etapa (c); en donde el ácido nucleico de replicón se introduce en dicha célula hospedante mediante electroporación; y en donde la densidad celular en el ámbito de la electroporación es de 107 a 5 x 108 por mL.

8. 发明专利

AT437222T 未知
公开(公告)号：AT437222T
公开(公告)日：2009-08-15
申请号：AT02763613
申请日：2002-09-06
申请人： ALPHAVAX INC
发明人： SMITH JONATHAN , KAMRUD KURT , RAYNER JONATHAN , DRYGA SERGEY , CALEY IAN
IPC分类号： C12N7/02 , C12N15/09 , A61K35/76 , A61K48/00 , A61P31/00 , A61P35/00 , A61P37/04 , C12N5/10 , C12N7/04 , C12N15/68 , C12N15/86
摘要： The present invention provides compositions useful in and methods for producing populations of infectious, replication-defective alphavirus replicon particles that contain no replication competent alphavirus particles, as determined by passage on cells in culture. The compositions include helper and replicon nucleic acid molecules that can further reduce the predicted frequency for formation of replication-competent virus and can optimize manufacturing strategies and costs.

9. 发明专利

AU2003297039A1 MULTI-ANTIGENIC ALPHAVIRUS REPLICON PARTICLES AND METHODS 未知
公开(公告)号：AU2003297039A1
公开(公告)日：2004-07-09
申请号：AU2003297039
申请日：2003-12-12
申请人： ALPHAVAX INC
发明人： SMITH JONATHAN F , KAMRUD KURT , DRYGA SERGEY , CALEY IAN
IPC分类号： A61K48/00 , C12N7/04 , C12N15/86

10. 发明专利

CA2509973A1 MULTI-ANTIGENIC ALPHAVIRUS REPLICON PARTICLES AND METHODS 未知
公开(公告)号：CA2509973A1
公开(公告)日：2004-07-01
申请号：CA2509973
申请日：2003-12-12
申请人： ALPHAVAX INC
发明人： CALEY IAN , SMITH JONATHAN F , KAMRUD KURT , DRYGA SERGEY
IPC分类号： A61K48/00 , C12N7/04 , C12N15/86 , C12N7/00 , A01N63/00 , C07H21/02 , C12N5/06 , C12N5/10 , C12N15/63
摘要： Viral replicon selected nucleic acid expression libraries are useful for analyzing multiple antigens associated with a parasite, pathogen or neoplasi a or for preparing immunogenic compositions for generating immune responses specific for the parasite pathogen or neoplasia. Alphavirus replicon particl es representative of the nucleic acid expression library are preferred. The nucleic acid library can be a random library, or it can be prepared after a selection step, for example, by differential hybridization prior to cloning into the replicon vector.

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