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1. 发明申请

WO2004044246A3 METHODS AND COMPOSITIONS FOR DETECTING TELOMERASE ACTIVITY 审中-公开
标题翻译：检测端粒酶活性的方法和组合物
公开(公告)号：WO2004044246A3
公开(公告)日：2004-10-21
申请号：PCT/US0335919
申请日：2003-11-12
申请人： ALLIED BIOTECH INC , LI ZHUANGWU , BAO JUN , MAO HUA , MA WENBIN , LI LINA
发明人： LI ZHUANGWU , BAO JUN , MAO HUA , MA WENBIN , LI LINA
IPC分类号： C12Q1/68 , C12Q1/48
CPC分类号： C12Q1/6886 , C12Q1/68 , C12Q1/6841 , C12Q1/6851 , C12Q1/686 , C12Q2561/113 , C12Q2547/107 , C12Q2521/113 , C12Q2547/101 , C12Q2545/113
摘要： A method for determining telomerase activity using primer extension followed with real time PCR quantification is disclosed. The method of the present invention provides a rapid, sensitive and accurate measurement for telomerase activity in a biological sample. In one embodiment, the method includes the steps of: adding the biological sample to a reaction tube containing a first reaction mixture having a first primer and nucleoside triphosphates, a second reaction mixture having a second primer and a DNA polymerase, and a wax layer that separates the first reaction mixture from the second reaction mixture; incubating the biological sample with the first reaction mixture; admixing the extension product with the second reaction mixture; amplifying and quantifying the extension product using real-time PCR and a control template. In another embodiment, the detection method includes an in situ primer extension step that allows the production of the extension product within an intact cell. In this embodiment, the extension product can be preserved under appropriate conditions for an extended time before the completion of the quantification step.
摘要翻译：公开了使用引物延伸，然后进行实时PCR定量来确定端粒酶活性的方法。本发明的方法提供了生物样品中端粒酶活性的快速，灵敏和准确的测量。在一个实施方案中，该方法包括以下步骤：将生物样品加入到含有第一反应混合物和蜡层的反应管中，所述第一反应混合物具有第一引物和三磷酸核苷，第二反应混合物具有第二引物和DNA聚合酶，将第一反应混合物与第二反应混合物分离; 将生物样品与第一反应混合物温育; 将延伸产物与第二反应混合物混合; 使用实时PCR和对照模板扩增和定量延伸产物。在另一个实施方案中，检测方法包括原位引物延伸步骤，其允许在完整细胞内产生延伸产物。在该实施方案中，延伸产物可以在定量步骤完成之前的适当条件下保存延长的时间。

2. 发明申请

WO2006061392A3 SUBSTITUTED TETRACYCLIC TETRAHYDROFURAN, PYRROLIDINE AND TETRAHYDROTHIOPHENE DERIVATIVES 审中-公开
标题翻译：取代的四氢四氢呋喃，吡咯烷和四氢苯并噻吩衍生物
公开(公告)号：WO2006061392A3
公开(公告)日：2006-11-02
申请号：PCT/EP2005056544
申请日：2005-12-06
申请人： JANSSEN PHARMACEUTICA NV , CID-NUNEZ JOSE MARIA , MEGENS ANTONIUS ADRIANUS HENDR , TRABANCO-SUAREZ ANDRES AVELINO , KOUKNI MOHAMED , HOORNAERT GEORGES JOSEPH CORNE , COMPERNOLLE FRANS JOSEF CORNEL , KOZLECKI TOMASZ , MAO HUA , JHA SUSHIL CHANDRA , FERNANDEZ-GADEA FRANCISCO JAVI
发明人： CID-NUNEZ JOSE MARIA , MEGENS ANTONIUS ADRIANUS HENDR , TRABANCO-SUAREZ ANDRES AVELINO , KOUKNI MOHAMED , HOORNAERT GEORGES JOSEPH CORNE , COMPERNOLLE FRANS JOSEF CORNEL , KOZLECKI TOMASZ , MAO HUA , JHA SUSHIL CHANDRA , FERNANDEZ-GADEA FRANCISCO JAVI
IPC分类号： C07D209/70 , A61K31/343 , A61K31/352 , A61K31/381 , A61K31/403 , A61K31/407 , A61K31/4188 , A61K31/496 , A61K31/4985 , C07D221/18 , C07D307/93 , C07D311/94 , C07D333/80 , C07D487/04 , C07D491/044
CPC分类号： C07D333/80 , C07D209/70 , C07D221/18 , C07D307/93 , C07D311/94 , C07D487/04 , C07D491/04
摘要： This invention concerns novel substituted tetracyclic tetrahydrofuran, pyrrolidine and tetrahydrothiophene derivatives with binding affinities towards serotonin receptors, in particular 5-HT 2A and 5-HT 2C receptors, and towards dopamine receptors, in particular dopamine D2 receptors and with norepinephrine reuptake inhibition properties, pharmaceutical compositions comprising the compounds according to the invention, the use thereof as a medicine, in particular for the prevention and/or treatment of a range of psychiatric and neurological disorders, in particular certain psychotic, cardiovascular and gastrokinetic disorders and processes for their production. The compounds according to the invention can be represented by general Formula (I) and comprises also the pharmaceutically acceptable acid or base addition salts thereof, the stereochemically isomeric forms thereof, the N-oxide form thereof and prodrugs thereof, wherein all substituents are defined as in Claim 1.
摘要翻译：本发明涉及对5-羟色胺受体，特别是5-HT 2A和5-HT 2C受体具有结合亲和力的新型取代四环四氢呋喃，吡咯烷和四氢噻吩衍生物，并且涉及多巴胺受体特别是多巴胺D2受体和去甲肾上腺素再摄取抑制性质，包含根据本发明的化合物的药物组合物，其作为药物的用途，特别是用于预防和/或治疗一系列精神和神经障碍，特别是某些精神病，心血管和胃肠功能障碍及其生产过程。根据本发明的化合物可以由通式（I）表示，并且还包括其药学上可接受的酸或碱加成盐，其立体化学异构形式，其N-氧化物形式及其前药，其中所有取代基定义为在权利要求1中。

3. 发明申请

WO2004003494A3 A METHOD OF AND APPARATUS FOR MEASURING OSCILLATORY MOTION 审中-公开
标题翻译：一种测量振动运动的方法和设备
公开(公告)号：WO2004003494A3
公开(公告)日：2004-05-13
申请号：PCT/US0320310
申请日：2003-06-25
申请人： BIOTECHPLEX CORP , WONG LID B , MAO HUA , CHANDRA TARUN , YEATES DONOVAN B
发明人： WONG LID B , MAO HUA , CHANDRA TARUN , YEATES DONOVAN B
IPC分类号： A61B5/11 , G01N21/47 , G02B6/26
CPC分类号： A61B5/1101 , A61B5/726 , A61B2503/40 , G01N21/4738
摘要： A method of measuring oscillatory motion of a sample is disclosed. The method comprises the steps of illuminating a sample with an illuminating optical fiber; detecting backscattered light from the sample with a plurality of detecting optical fibers; coupling each optical fiber of the plurality of detecting optical fibers with a modulating optical fiber; and generating measurements of the oscillatory motion of the sample. An apparatus for measuring oscillatory motion of a sample is also disclosed. The apparatus comprises a light source; an illuminating optical fiber coupled to the light source; and a plurality of optical fibers positioned around the illuminating optical fiber and coupled to receive backscattered light from the sample.
摘要翻译：公开了一种测量样本振荡运动的方法。该方法包括以下步骤：用照射光纤照射样品; 用多根检测光纤检测来自样品的背散射光; 将多根检测光纤中的每根光纤与调制光纤耦合; 并产生样本振荡运动的测量结果。还公开了一种用于测量样本的振荡运动的设备。该设备包括光源; 耦合到光源的照明光纤; 以及定位在照射光纤周围并耦合以接收来自样本的背散射光的多个光纤。

4. 发明申请

WO03048147A2 PREPARATION OF CIS-FUSED 3,3a,8,12b-TETRAHYDRO-2H-DIBENZO[3,4:6,7]CYCLOHEPTA[1,2-b]FURAN DERIVATIVES 审中-公开
标题翻译：独立制备3,3a，8,12b-四氢-2H-二苯并[3,4：6,7]环己基[1,2-b]呋喃衍生物的制备
公开(公告)号：WO03048147A2
公开(公告)日：2003-06-12
申请号：PCT/EP0213561
申请日：2002-12-02
申请人： JANSSEN PHARMACEUTICA NV , MAO HUA , KOZLECKI TOMASZ , COMPERNOLLE FRANS JOSEF CORNEL , HOORNAERT GEORGES JOSEPH CORNE
发明人： MAO HUA , KOZLECKI TOMASZ , COMPERNOLLE FRANS JOSEF CORNEL , HOORNAERT GEORGES JOSEPH CORNE
IPC分类号： A61K31/34 , A61K31/343 , A61P25/00 , A61P25/06 , A61P25/18 , A61P25/22 , A61P25/24 , A61P25/30 , C07D307/93
CPC分类号： C07D307/93
摘要： The present invention concerns a process for preparing each of the 4 individual diastereomers of formula (I) in stereochemically pure form from a single enantiomerically pure precursor. The tetracyclic ringsystem having cis-fused five and seven membered rings is formed in a base-catalysed cyclization reaction. The invention further relates to the thus obtained cis-fused tetracyclic alcohol intermediates and methanamine end-products, and the methanamine end-products for use as a medicine, in particular as CNS active medicines.
摘要翻译：本发明涉及从单一对映异构体纯的前体以立体化学纯的形式制备式（I）的4种单独的非对映异构体的方法。在碱催化的环化反应中形成具有顺式稠合的五元和七元环的四环环系。本发明还涉及由此获得的顺式稠合四环醇中间体和甲胺终产物，以及用作药物的甲胺最终产物，特别是CNS活性药物。

5. 发明专利

AU2003290722A8 Methods and compositions for detecting telomerase activity 未知
公开(公告)号：AU2003290722A8
公开(公告)日：2004-06-03
申请号：AU2003290722
申请日：2003-11-12
申请人： ALLIED BIOTECH INC
发明人： LI ZHUANGWU , MAO HUA , MA WENBIN , BAO JUN , LI LINA
IPC分类号： C12Q1/68 , C12Q1/48

6. 发明专利

AU2003290722A1 METHODS AND COMPOSITIONS FOR DETECTING TELOMERASE ACTIVITY 未知
公开(公告)号：AU2003290722A1
公开(公告)日：2004-06-03
申请号：AU2003290722
申请日：2003-11-12
申请人： ALLIED BIOTECH INC
发明人： LI ZHUANGWU , BAO JUN , MAO HUA , MA WENBIN , LI LINA
IPC分类号： C12Q1/68

7. 发明专利

CA2505871A1 METHODS AND COMPOSITIONS FOR DETECTING TELOMERASE ACTIVITY 未知
公开(公告)号：CA2505871A1
公开(公告)日：2004-05-27
申请号：CA2505871
申请日：2003-11-12
申请人： ALLIED BIOTECH INC
发明人： MA WENBIN , LI ZHUANGWU , BAO JUN , MAO HUA , LI LINA
IPC分类号： C12Q1/68 , C12Q1/48
摘要： A method for determining telomerase activity using primer extension followed with real time PCR quantification is disclosed. The method of the present invention provides a rapid, sensitive and accurate measurement for telomeras e activity in a biological sample. In one embodiment, the method includes the steps of: adding the biological sample to a reaction tube containing a first reaction mixture having a first primer and nucleoside triphosphates, a secon d reaction mixture having a second primer and a DNA polymerase, and a wax laye r that separates the first reaction mixture from the second reaction mixture; incubating the biological sample with the first reaction mixture; admixing t he extension product with the second reaction mixture; amplifying and quantifyi ng the extension product using real-time PCR and a control template. In another embodiment, the detection method includes an in situ primer extension step that allows the production of the extension product within an intact cell. I n this embodiment, the extension product can be preserved under appropriate conditions for an extended time before the completion of the quantification step.

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