会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 5. 发明专利
    • DE1442193A1
    • 1968-11-21
    • DE1442193
    • 1963-07-13
    • AJINOMOTO KK
    • IIZUKA HIROSHIKATSUYA NOBORUSHILIO ISAMUKOMAGATA KAZUOOTSUKA SHINICHIROISHII RYOSUKE
    • C12P13/04C12P13/14
    • Amino acids such as glutanic acid, aspartic acid, alanine, phenylalanine, lysine, arginine, tyrosine, isolevicene, valine, glycine, threonine, tryptophan, proline and serine are prepared by cultivating a microorganism in a medium containing a hydrocarbon, a nitrogen source, inorganic substances and, if desired, growth-promoting substances under aerobic conditions, said microorganism being one which is capable of growing on one of the following media, viz. (a) a medium containing keronsine 10g., NH4Cl 2.0g., Na2HPO4.12H2O 1.0g., KH2PO4 1.5g., MgSO4.7H2O 0.5g., NaCl 2.0g., and 1 litre of distilled water; (b) a medium containing crude petroleum 10g., NH4NO3 2.5g., Na2HPO4 12H2O 1.0g., KH2PO4 1.5g., MgSO4.7H2O 1.5g., MnCl2 0.2g., CaCl2 trace, FeSO4.7H2O trace, MoSO4 trace, CaCO3 (separately sterilized) 0.5g., and 1 litre of distilled water; (c) a medium containing dissolved methane and/or propane, NH4Cl 1.0g., KH2PO4 0.5., MgSO4 0.2g., FeCl3 trace, and 1 litre of distilled water, which medium is in contact with a gas mixture of air and methane and/or propane the ratio of air: methane and/or propane being 1 : 1; or (d) a medium comprising either of mediums (a), (b) or (c) together with a growth promoting substance. Suitable hydrocarbon sources are liquid paraffin, kerosine, light oil, heavy oil, naphtha, ligroin, propane, butane, propylene, ethane, methane, natural gas or a mixture thereof. Specified microorganisms are Corynebacterium oleophilus and hydrocarboclastus; Brevibacterium fulrum, acetylicum, albus and cerinus; Achromobacter pestifer; Pseudomonas ovalis; Escherichia coli; Mycobacterium brevicale; Candida lipolytica and tropicalis and Hansenula anomala.