会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 3. 发明申请
    • GENETIC CONTROL OF ETHYLENE BIOSYNTHESIS IN PLANTS
    • 植物中乙烯生物合成的遗传控制
    • WO1991009112A1
    • 1991-06-27
    • PCT/US1990007175
    • 1990-12-12
    • AGRITOPE, INC.FERRO, Adolph, J.BESTWICK, Richard, K.BROWN, Lyle, R.
    • AGRITOPE, INC.
    • C12N01/20
    • C12N15/8249C12N9/14
    • A method for control of ethylene biosynthesis in plants comprising a vector containing a selective gene under plant promoter control, and a DNA insert comprising codons for a functional heterologous polypeptide having AdoMetase activity and flanked by a plant promoter on one side and a polyA signal sequence on the other side; and, transforming a plant host with said vector wherein the plant host transformed thereby is capable of expressing the heterologous polypeptide having AdoMetase activity under the control of said control region. The presence and expression of AdoMetase in transgenic plants lowers AdoMet levels and generates an inhibitor of ACC synthase causing a corresponding decrease in ethylene biosynthesis and precursor availability. The current construction of transgenic plants containing a copy(s) of the T3 AdoMetase gene allow for construction of plants that will control ethylene biosynthesis resulting in fruits, vegetables, and flowers which have improved shelf life and preservation qualities.
    • 一种用于在植物中控制乙烯生物合成的方法,其包含在植物启动子控制下含有选择性基因的载体,以及DNA插入片段,其包含具有AdoMetase活性的功能性异源多肽的密码子,并且侧面有一侧的植物启动子和polyA信号序列 另一边; 并且用所述载体转化植物宿主,其中由此转化的植物宿主能够在所述对照区域的控制下表达具有AdoMetase活性的异源多肽。 AdoMetase在转基因植物中的存在和表达降低AdoMet水平,并产生ACC合酶抑制剂,导致乙烯生物合成和前体可利用率相应降低。 目前,含有T3 AdoMetase基因拷贝的转基因植物的构建允许构建能控制乙烯生物合成的植物,从而导致水果,蔬菜和鲜花具有改善的保质期和保存性质。