专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO9830883A2 ANALYSIS OF GENETIC POLYMORPHISMS AND GENE COPY NUMBER 审中-公开
标题翻译：遗传多态性分析及遗传复制数
公开(公告)号：WO9830883A2
公开(公告)日：1998-07-16
申请号：PCT/US9806414
申请日：1998-01-02
申请人： AFFYMETRIX INC , CRONIN MAUREEN T , MIYADA CHARLES G , HUBBELL EARL A , CHEE MARK , FODOR STEPHEN P A , HUANG XIAOHUA C , LIPSHUTZ ROBERT J , LOBBAN PETER E , MORRIS MACDONALD S , SHELDON EDWARD L
发明人： CRONIN MAUREEN T , MIYADA CHARLES G , HUBBELL EARL A , CHEE MARK , FODOR STEPHEN P A , HUANG XIAOHUA C , LIPSHUTZ ROBERT J , LOBBAN PETER E , MORRIS MACDONALD S , SHELDON EDWARD L
IPC分类号： B01J19/00 , C07B61/00 , C07H21/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00644 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07H21/00 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , C12Q1/6876 , C12Q1/6883 , C12Q2600/106 , C12Q2600/156 , C40B40/06 , C40B60/14 , C12Q2565/513
摘要： The invention provides methods for detecting variations in polymorphic sites and/or variations in gene copy number. The methods are particularly useful for analysis of biotransformation genes, such as cytochromes P450.
摘要翻译：本发明提供了用于检测多态位点变异和/或基因拷贝数变异的方法。该方法对生物转化基因如细胞色素P450的分析特别有用。

2. 发明申请

WO0011223A9 USE OF POOLED PROBES IN GENETIC ANALYSIS 审中-公开
标题翻译：在遗传分析中使用沉积探针
公开(公告)号：WO0011223A9
公开(公告)日：2001-04-12
申请号：PCT/US9919069
申请日：1999-08-19
申请人： AFFYMETRIX INC , GENTALEN ERIK , CHEE MARK
发明人： GENTALEN ERIK , CHEE MARK
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , G01N33/566 , G01N37/00
CPC分类号： C12Q1/6837 , C12Q1/6827 , C12Q1/6874 , C12Q2565/543 , C12Q2565/513
摘要： The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provides methods of using such arrays fo r e.g., linkage analysis, sequence analysis, and expression monitoring.
摘要翻译：本发明提供具有至少一个合并位置的多核苷酸探针阵列。典型的阵列包括具有至少三个离散区域的支撑件。第一区域具有包含第一和第二探针的多核苷酸探针池。第二区域承载第一探针而没有第二探针，第三区域承载第二探针而没有第一探针。具有与第一和第二探针两者互补的区段的靶核酸由于在第一区域中的合并探针的协同结合而显示比第一区域更强的标准化结合，而不是与第二和第三区域结合的聚集体。本发明提供了使用这种阵列的方法，例如连锁分析，序列分析和表达监测。

3. 发明专利

JPH1099085A POLYMORPHISM OF HUMAN MITOCHONDRIAL DNA 失效
公开(公告)号：JPH1099085A
公开(公告)日：1998-04-21
申请号：JP16320397
申请日：1997-05-16
申请人： AFFYMETRIX INC
发明人： CHEE MARK , BERNO ANTHONY , YANG ROBERT
IPC分类号： C12N15/09 , C12Q1/68 , G01N21/64 , G02B21/00
摘要： PROBLEM TO BE SOLVED: To obtain the subject nucleic acid, comprising a segment, etc., of a human mitochondrial. DNA or RNA of a specific number of bases including any one of polymorphoric sites, capable of detecting the polymorphism of the mitochondria and useful for forensic analysis, diagnosis, etc., of diseases from a correlation thereof with phenotypes. SOLUTION: This new nucleic acid comprises a segment of a human mitochondrial DNA or RNA of between 10 and 100 bases including any one of polymorphic sites or a complement of the segment. The polymorphism can be utilized in the forensic analysis for determining whether or not a blood sample of a suspect matches a polymorphic form of a blood or other tissue samples from a crime scene by detecting the mitochondrial polymorphic form or the diagnosis, etc., of diseases from inherited disease traits from a correlation of phenotypic traits with the polymorphism. The nucleic acid is obtained by cleaving the human mitochondrial DNA or RNA into segments of between 10 and 100 bases including any one of 182 polymorphic sites.

4. 发明专利

JP2004166716A Method for monitoring allele expression by detecting genetic polymorphism with probe array 审中-公开
公开(公告)号：JP2004166716A
公开(公告)日：2004-06-17
申请号：JP2004075405
申请日：2004-03-16
申请人： Affymetrix Inc , アフィメトリックスインコーポレイテッド
发明人： CHEE MARK
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2600/156 , C12Q2600/158 , Y10S435/911 , C12Q2535/131
摘要： PROBLEM TO BE SOLVED: To provide a method for monitoring expression level of polymorphism having different gene.
SOLUTION: The method for monitoring expression level of polymorphism having different gene comprises a step for analyzing genome DNA of an individual to determine existence of a heterozygous polymorphism form in a polymorphism part in a transcribed sequence in the objective gene and a step for analyzing RNA from organisms of the individual in which step the gene is expressed and the relative ratio of the polymorphism form in the transcribed sequence of the gene is determined.
COPYRIGHT: (C)2004,JPO

5. 发明专利

JP2006191932A Method for nucleic acid analysis 审中-公开
标题翻译：核酸分析方法
公开(公告)号：JP2006191932A
公开(公告)日：2006-07-27
申请号：JP2006035873
申请日：2006-02-13
申请人： Affymetrix Inc , アフィメトリックスインコーポレイテッド
发明人： LOCKHART DAVID J , CHEE MARK , GUNDERSON KEVIN , CHAOQIANG LAI , WODICKA LISA , CRONIN MAUREEN T , LEE DANNY , TRAN HUU M , MATSUZAKI HAJIME , MCGALL GLENN H , BARONE ANTHONY D
IPC分类号： C12Q1/68 , G01N37/00 , C07B61/00 , C07H19/052 , C07H19/12 , C07H21/00 , C12N15/09 , G01N33/58 , G06F17/30
CPC分类号： C12Q1/6809 , C07H19/052 , C07H19/12 , C07H21/00 , C12Q1/6816 , C12Q1/6837 , C12Q2600/156 , C40B40/00 , C12Q2565/501 , C12Q2521/501
摘要： PROBLEM TO BE SOLVED: To provide a method for monitoring expression of multiple screened genes. SOLUTION: The method for identifying differences of nucleic acid level between two or more samples comprises steps of: (a) providing one or more oligonucleotide arrays containing a probe oligonucleotide added on the surface; (b) hybridizing a nucleic acid sample with the above-mentioned one one more arrays to form a hybrid double bond between the nucleic acid in the nucleic acid sample and a probe oligonucleotide in the above-mentioned one or more array complementary to a subsequence thereof; (c) contacting the above-mentioned one or more arrays with a nucleic acid ligase; and (d) indicating the difference of nucleic acid levels with the differences of hybridization to determine the hybridization difference between nucleic acid samples. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种监测多重筛选基因表达的方法。解决方案：鉴定两个或多个样品之间的核酸水平差异的方法包括以下步骤：（a）提供一种或多种含有添加在表面上的探针寡核苷酸的寡核苷酸阵列; （b）将核酸样品与上述一个多个阵列杂交以在核酸样品中的核酸与上述与其子序列互补的一个或多个阵列中的探针寡核苷酸之间形成杂交双键 ; （c）使上述一个或多个阵列与核酸连接酶接触; 和（d）表示核酸水平与杂交差异的差异，以确定核酸样品之间的杂交差异。版权所有（C）2006，JPO＆NCIPI

6. 发明申请

WO2004007755A9 MULTIPLEX NUCLEIC ACID REACTIONS 审中-公开
标题翻译：多重核酸反应
公开(公告)号：WO2004007755A9
公开(公告)日：2004-05-06
申请号：PCT/US0322171
申请日：2003-07-15
申请人： ILLUMINA INC , CHEE MARK , FAN JIAN-BING , GUNDERSON KEVIN
发明人： CHEE MARK , FAN JIAN-BING , GUNDERSON KEVIN
IPC分类号： C12Q1/68 , C12Q
CPC分类号： C12Q1/6858 , C12Q2563/179 , C12Q2563/149 , C12Q2535/125
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously. The invention provides a method of detecting a target sequence. The method consists of: (a) contacting a first and second probe with a target sequence under conditions where complementary probes form a hybridization complex with the target sequence, the first probe comprising an upstream universal priming site and a target specific sequence, the second probe comprising a downstream universal priming site and a target-specific sequence, wherein one of the first or second probes comprise an adapter sequence; (b) extending the first or second probe of the hybridization complex to form a modified probe; (c) amplifying the modified probe to form an amplicon, and (d) detecting the amplicon. A method of detecting the relative amounts of two or more target sequences is also provided. The method consists of (a) contacting a first and a second probe with first and second target sequences in an initial population under conditions where complementary probes form a hybridization complex with the target sequences, the first and second probes comprising a universal priming site, an adapter sequence and a target-specific sequence; (b) linearly amplifying the first and second probes forming the hybridization complex to produce first and second amplicons having distinctive adapter sequences, and (c) determining a relative amount of the first and second amplicons distinguishable by the adapter sequence, wherein the relative amount of the amplicons is indicative of the relative amounts of the first and second target sequences in the initial population. Further provided is a method of amplifying a target sequence to produce a signal within a dynamic range of a detection assay. The method consists of: (a) hybridizing a target-specific probe having an upstream universal priming site (UUP), a downstream universal priming site (DUP) and an adapter sequence with a set of differential primers, the set of differential primers comprising an upstream primer and first and second downstream primers, the second downstream primer having a lower Tm compared to the upstream primer and the first downstream primer; (b) amplifying the probe with the set of differential primers for two or more cycles of enzymatic polymerization; (c) increasing hybridization stringency to suppress hybridization of the second downstream primer, and (d) amplifying the probe from the upstream and the first downstream primers of the set for at least one cycle of enzymatic polymerization, wherein differential signals of amplicons produced from amplification of the first or the second downstream primers fall within a dynamic range of a detection assay.
摘要翻译：本发明涉及用于同时扩增和/或基因型多种样品的各种复用方法。本发明提供一种检测靶序列的方法。该方法包括：（a）在互补探针与靶序列形成杂交复合物的条件下使第一和第二探针与靶序列接触，第一探针包含上游通用引发位点和靶特异性序列，第二探针包括下游通用引发位点和靶特异性序列，其中所述第一或第二探针中的一个包含衔接子序列; （b）扩增杂交复合物的第一或第二探针以形成修饰的探针; （c）扩增经修饰的探针以形成扩增子，和（d）检测扩增子。还提供了检测两个或多个靶序列的相对量的方法。该方法包括（a）在互补探针与靶序列形成杂交复合物的条件下，在初始群体中使第一和第二探针与第一和第二靶序列接触，第一和第二探针包含通用引发位点，衔接子序列和目标特异性序列; （b）线性扩增形成杂交复合物的第一和第二探针以产生具有不同衔接序列的第一和第二扩增子，和（c）确定由衔接子序列可区分的第一和第二扩增子的相对量，其中相对量扩增子表示初始群体中第一和第二靶序列的相对量。还提供了扩增靶序列以在检测测定的动态范围内产生信号的方法。该方法包括：（a）将具有上游通用引物位点（UUP），下游通用引物位点（DUP）和衔接子序列的靶特异性探针与一组差异引物杂交，所述差异引物组包含上游引物和第一和第二下游引物，所述第二下游引物与上游引物和第一下游引物相比具有较低的Tm; （b）用该组差异引物扩增探针两个或多个酶促聚合循环; （c）增加杂交严格性以抑制第二下游引物的杂交，和（d）从酶的聚合的至少一个循环的上游和第一个下游引物扩增探针，其中扩增产生的扩增子的差异信号的第一或第二下游引物落在检测测定的动态范围内。

7. 发明申请

WO2004007755A2 MULTIPLEX NUCLEIC ACID REACTIONS 审中-公开
公开(公告)号：WO2004007755A2
公开(公告)日：2004-01-22
申请号：PCT/US0322171
申请日：2003-07-15
申请人： ILLUMINA INC , CHEE MARK , FAN JIAN-BING , GUNDERSON KEVIN
发明人： CHEE MARK , FAN JIAN-BING , GUNDERSON KEVIN
IPC分类号： C12Q1/68 , C12Q
CPC分类号： C12Q1/6858 , C12Q2563/179 , C12Q2563/149 , C12Q2535/125
摘要： The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously. The invention provides a method of detecting a target sequence. The method consists of: (a) contacting a first and second probe with a target sequence under conditions where complementary probes form a hybridization complex with the target sequence, the first probe comprising an upstream universal priming site and a target specific sequence, the second probe comprising a downstream universal priming site and a target-specific sequence, wherein one of the first or second probes comprise an adapter sequence; (b) extending the first or second probe of the hybridization complex to form a modified probe; (c) amplifying the modified probe to form an amplicon, and (d) detecting the amplicon. A method of detecting the relative amounts of two or more target sequences is also provided. The method consists of (a) contacting a first and a second probe with first and second target sequences in an initial population under conditions where complementary probes form a hybridization complex with the target sequences, the first and second probes comprising a universal priming site, an adapter sequence and a target-specific sequence; (b) linearly amplifying the first and second probes forming the hybridization complex to produce first and second amplicons having distinctive adapter sequences, and (c) determining a relative amount of the first and second amplicons distinguishable by the adapter sequence, wherein the relative amount of the amplicons is indicative of the relative amounts of the first and second target sequences in the initial population. Further provided is a method of amplifying a target sequence to produce a signal within a dynamic range of a detection assay. The method consists of: (a) hybridizing a target-specific probe having an upstream universal priming site (UUP), a downstream universal priming site (DUP) and an adapter sequence with a set of differential primers, the set of differential primers comprising an upstream primer and first and second downstream primers, the second downstream primer having a lower Tm compared to the upstream primer and the first downstream primer; (b) amplifying the probe with the set of differential primers for two or more cycles of enzymatic polymerization; (c) increasing hybridization stringency to suppress hybridization of the second downstream primer, and (d) amplifying the probe from the upstream and the first downstream primers of the set for at least one cycle of enzymatic polymerization, wherein differential signals of amplicons produced from amplification of the first or the second downstream primers fall within a dynamic range of a detection assay.

8. 发明专利

DE69918130T2 未知
公开(公告)号：DE69918130T2
公开(公告)日：2005-07-07
申请号：DE69918130
申请日：1999-08-19
申请人： AFFYMETRIX INC
发明人： GENTALEN ERIK , CHEE MARK
IPC分类号： G01N33/53 , C12M1/00 , C12M1/34 , C12N15/09 , C12Q1/68 , C12Q1/6827 , C12Q1/6837 , C12Q1/6874 , G01N33/566 , G01N37/00
摘要： The invention provides arrays of polynucleotide probes having at least one pooled position. A typical array comprises a support having at least three discrete regions. A first region bears a pool of polynucleotide probes comprising first and second probes. A second region bears the first probe without the second probe and a third region bears the second probe without the first probe. A target nucleic acid having segments complementary to both the first and second probes shows stronger normalized binding to the first region than to the aggregate of binding to the second and third regions due to cooperative binding of pooled probes in the first region. The invention provides methods of using such arrays for e.g., linkage analysis, sequence analysis, and expression monitoring.

9. 发明专利

DE69830395D1 未知
公开(公告)号：DE69830395D1
公开(公告)日：2005-07-07
申请号：DE69830395
申请日：1998-03-19
申请人： AFFYMETRIX INC N D GES D STAAT
发明人： CHEE MARK
IPC分类号： C12Q1/68
摘要： The invention provides iterative methods of analyzing a target nucleic acid that represents a variant of a reference nucleic acid. An array of probes is designed to be complementary to an estimated sequence of a target nucleic acid. The array of probes is then hybridized to the target nucleic acid. The target sequence is reestimated from hybridization pattern of the array to the target nucleic acid. A further array of probes is then designed to be complementary to the reestimated sequence, and this array is used to obtain a further reestimate of the sequence of the target nucleic acid. By performing iterative cycles of array design and target sequence estimation, the estimated sequence of the target converges with the true sequence.

10. 发明专利

AU751557B2 Expression monitoring by hybridization to high density oligonucleotide arrays 未知
公开(公告)号：AU751557B2
公开(公告)日：2002-08-22
申请号：AU5364900
申请日：2000-08-25
申请人： AFFYMETRIX INC
发明人： LOCKHART DAVID J , BROWN EUGENE L , WONG GORDON , CHEE MARK , GINGERAS THOMAS R , MITTMANN MICHAEL P , LIPSHUTZ ROBERT J , FODOR STEPHEN P A , WANG CHUNWEI
IPC分类号： C12Q1/68

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式