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1. 发明申请

WO2004056995A1 PROCESS FOR PRODUCING AN IN VITRO PEPTIDE EXPRESSION LIBRARY 审中-公开
标题翻译：生产体外肽表达图谱的方法
公开(公告)号：WO2004056995A1
公开(公告)日：2004-07-08
申请号：PCT/GB2002/005909
申请日：2002-12-23
申请人： AFFITECH AS , OWEN, Deborah , MARVIK, Ole, Jørgen , REIERSEN, Herald , BREKKE, Ole, Henrik
发明人： MARVIK, Ole, Jørgen , REIERSEN, Herald , BREKKE, Ole, Henrik
IPC分类号： C12N15/10
CPC分类号： C12N15/1075
摘要： The present invention provides a method of producing a peptide expression library which displays a plurality of different peptides, wherein displayed peptides coupled to the genetic material encoding them, said method comprising the following steps: (a) preparing a library of nucleic acid molecules wherein each member of the library comprises a nucleotide sequence encoding a nucleic acid binding amino acid sequence, an oligonucleotide tag and a nucleotide sequence encoding the peptide which is to be displayed said nucleic acid binding sequence being capable of coupling to the nucleic acid molecule which encodes it; (b) attaching said library or a subpopulation thereof to one or more solid supports, wherein said attachment occurs by specific hybridisation of the oligonucleotide tag of a library member to a complementary tag which is attached to a solid support or a discrete area of a solid support so that each solid support or discrete area of a solid support has attached a distinct type of individual library members; and (c) translating the library members. Nucleic acid and peptide expression libraries and the members thereof form yet further aspects of the invention, as do kits comprising these libraries and members thereof. Further aspects involve the use of said libraries to screen and identify peptides or proteins or other entities exhibiting desired properties or the use of said library to screen against another library.
摘要翻译：本发明提供了一种产生肽表达文库的方法，其显示多个不同的肽，其中显示的肽与编码它们的遗传物质偶联，所述方法包括以下步骤：（a）制备核酸分子文库，其中每个文库成员包括编码核酸结合氨基酸序列的核苷酸序列，寡核苷酸标签和编码该肽的核苷酸序列，该核苷酸序列将显示所述核酸结合序列，其能够与编码该核酸结合序列的核酸分子偶联; （b）将所述文库或其亚群连接至一种或多种固体支持物，其中所述附着通过文库成员的寡核苷酸标签与附着于固体支持物或固体的离散区域的互补标签的特异性杂交而发生支撑，使得固体支持物的每个固体支持物或离散区域附着有不同类型的单个文库成员; 和（c）翻译图书馆成员。核酸和肽表达文库及其成员形成本发明的进一步方面，以及包含这些文库及其成员的试剂盒。进一步的方面涉及使用所述文库来筛选和鉴定显示所需性质的肽或蛋白质或其他实体或使用所述文库筛选另一文库。

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