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1. 发明专利

AU3803001A Detection of single nucleotide polymorphisms 未知
公开(公告)号：AU3803001A
公开(公告)日：2001-08-14
申请号：AU3803001
申请日：2001-02-02
申请人： ADVION BIOSCIENCES INC
发明人： SCHULTZ GARY A , ZHANG SHENG , PELT COLLEEN K VAN
IPC分类号： C12Q1/68
摘要： The present invention relates to a method of detecting single nucleotide polymorphisms by providing a target nucleic acid molecule, an oligonucleotide primer complementary to a portion of the target nucleic acid molecule, a nucleic acid polymerizing enzyme, and a plurality of types of nucleotide analogs. The target nucleic molecule, the oligonucleotide primer, the nucleic acid polymerizing enzyme, and the nucleotide analogs, each type being present in a first amount, are blended to form an extension solution where the oligonucleotide primer is hybridized to the target nucleic acid molecule to form a primed target nucleic acid molecule and the nucleic acid polymerizing enzyme is positioned to add nucleotide analogs to the primed target nucleic acid molecule at an active site. The oligonucleotide primer in the extension solution is extended by using the nucleic acid polymerizing enzyme to add a nucleotide analog to the oligonucleotide primer at the active site. This forms an extended oligonucleotide primer, wherein the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid molecule at the active site. The amounts of each type of the nucleotide analogs in the extension solution after the extending step are then determined where each type is present in a second amount. The first and second amounts of each type of the nucleotide analog are compared. The type of nucleotide analog where the first and second amounts differ as the nucleotide added to the oligonucleotide primer at the active site is then identified. The steps of extending, determining the amounts of each type of the nucleotide analog, comparing the first and second amounts of the nucleotide analog, and said identifying the type of nucleotide analog added may be repeated, either after repeating the blending with the extended oligonucleotide primer or after determining the amounts of each type of dideoxynucleotide or dideoxynucleotide analog remaining in the extension solution as the new first amount. As a result, the nucleotide at the active site of the target nucleic acid molecule is determined. Also disclosed is an apparatus and composition for carrying out this method.

2. 发明专利

AU3292302A A one-well assay for high throughput detection of single nucleotide polymorphisms 未知
公开(公告)号：AU3292302A
公开(公告)日：2002-05-06
申请号：AU3292302
申请日：2001-10-27
申请人： ADVION BIOSCIENCES INC
发明人： ZHANG SHENG , PELT COLLEEN K VAN , SCHULTZ GARY A
IPC分类号： C12Q1/68

3. 发明专利

CA2438247A1 A MICROCHIP ELECTROSPRAY DEVICE AND COLUMN WITH AFFINITY ADSORBENTS AND USE OF THE SAME 未知
公开(公告)号：CA2438247A1
公开(公告)日：2002-08-29
申请号：CA2438247
申请日：2002-02-19
申请人： ADVION BIOSCIENCES INC
发明人： ZHANG SHENG , HUANG XIAN
IPC分类号： G01N27/62 , B01D15/38 , B01J20/281 , B01J20/32 , B01J45/00 , B05B5/08 , C12M1/00 , C12N15/09 , G01N30/26 , G01N30/46 , G01N30/52 , G01N30/56 , G01N30/60 , G01N30/72 , G01N30/88 , G01N33/15 , H01J49/04 , B01D15/08
摘要： A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array.(2) and a capillary tube (1) or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modern life sciences such as proteomics and drug discover.

4. 发明申请

WO0234883A3 A ONE-WELL ASSAY FOR HIGH THROUGHPUT DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：高通量检测单核苷酸多态性的一种检测方法
公开(公告)号：WO0234883A3
公开(公告)日：2002-11-07
申请号：PCT/US0150857
申请日：2001-10-27
申请人： ADVION BIOSCIENCES INC
发明人： ZHANG SHENG , VAN PELT COLLEEN K , SCHULTZ GARY A
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2535/125 , C12Q2531/113 , C12Q2521/525 , C12Q2565/501
摘要： The present invention relates to a novel one-well assay which couples a conventional polymerase chain reaction (PCR) amplification step to a single nucleotide primer extension step for the the determination of nucleotide sequence variations in the genotyping of single nucleotide polymorphisms and other DNA variations detected by primer extension methods. A PCR amplification step, a phosphatase digestion step (or a molecular weight-selective filter step), and a primer extension step are consecutively performed in the same well plate followed by electrospray mass spectrometry detection of a single nucleotide polymorphism bases. Alternative one-well assays which utilize exonuclease I or lambda -exonuclease in addition to the phosphatase digestion step are also disclosed.
摘要翻译：本发明涉及将常规聚合酶链式反应（PCR）扩增步骤偶联至单核苷酸引物延伸步骤以用于确定单核苷酸多态性和检测到的其他DNA变异的基因型分析中的核苷酸序列变异的新颖单孔试验通过引物延伸方法。在同一孔板中连续进行PCR扩增步骤，磷酸酶消化步骤（或分子量选择性过滤步骤）和引物延伸步骤，接着通过电喷雾质谱检测单核苷酸多态性碱基。也公开了除了磷酸酶消化步骤之外还使用外切核酸酶I或λ-外切核酸酶的替代性单孔测定法。

5. 发明公开

EP1363714A4 A MICROCHIP ELECTROSPRAY DEVICE AND COLUMN WITH AFFINITY ADSORBENTS AND USE OF THE SAME 有权
标题翻译： MICROCHIP电动喷雾装置和列AFFINITÄTSADSORPTIONSMITTELN及其用途
公开(公告)号：EP1363714A4
公开(公告)日：2007-05-09
申请号：EP02717446
申请日：2002-02-19
申请人： ADVION BIOSCIENCES INC
发明人： ZHANG SHENG , HUANG XIAN
IPC分类号： G01N27/62 , G01N30/72 , B01D15/08 , B01D15/38 , B01J20/281 , B01J20/32 , B01J45/00 , B05B5/08 , C12M1/00 , C12N15/09 , G01N30/26 , G01N30/46 , G01N30/52 , G01N30/56 , G01N30/60 , G01N30/88 , G01N33/15 , H01J49/04
CPC分类号： G01N30/6095 , B01D15/3804 , B01J20/28042 , B01J20/285 , B01J20/286 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3244 , B01J20/3251 , B01J20/3274 , B01J45/00 , B01J2220/52 , B01J2220/54 , B01J2220/58 , B01J2220/82 , G01N30/463 , G01N30/466 , G01N30/482 , G01N30/7266 , G01N2030/528 , H01J49/0018 , H01J49/167
摘要： A microchip-based electrospray ionization device and column with affinity adsorbents is disclosed. The invention includes a microchip array.(2) and a capillary tube (1) or alone or attached in combination. At least a portion of the device or column has immobilized affinity adsorbents. Methods for using the device are provided as well for affinity capture of biomolecules to meet the needs for the modern life sciences such as proteomics and drug discover.

6. 发明公开

EP1252336A4 DETECTION OF SINGLE NUCLEOTIDE POLYMORPHISMS 审中-公开
标题翻译：单检测
公开(公告)号：EP1252336A4
公开(公告)日：2005-02-09
申请号：EP01910423
申请日：2001-02-02
申请人： ADVION BIOSCIENCES INC
发明人： SCHULTZ GARY A , ZHANG SHENG , VAN PELT COLLEEN K
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , B01J2219/00274 , C12Q1/6869 , H01J49/165 , C12Q2565/627
摘要： The present invention relates to a method of detecting single nucleotide polymorphisms by providing a target nucleic acid molecule, an oligonucleotide primer complementary to a portion of the target nucleic acid molecule, a nucleic acid polymerizing enzyme, and a plurality of types of nucleotide analogs. The target nucleic molecule, the oligonucleotide primer, the nucleic acid polymerizing enzyme, and the nucleotide analogs, each type being present in a first amount, are blended to form an extension solution where the oligonucleotide primer is hybridized to the target nucleic acid molecule to form a primed target nucleic acid molecules and the nucleic acid polymerizing enzyme is positioned to add nucleotide analogs to the prime target nucleic acid molecule at an active site. The oligonucleotide primer in the extension solution is extended by using the nucleic acid polymerizing enzyme to add a nucleotide analog to the oligonucleotide primer at the active site. This forms an extended oligonucleotide primer, wherein the nucleotide analog being added is complementary to the nucleotide of the target nucleic acid molecule at the active site. The amounts of each type of the nucleotide analogs in the extension solution after the extending step are then determined where each type is present in a second amount. The first and second amounts of each type of the nucleotide analog are compared. The type of nucleotide analog where the first and second amounts differ as the nucleotide added to the oligonucleotide primer at the active site is then identified. The steps of extending, determining the amounts of each type of the nucleotide analog, comparing the first and second amounts of the nucleotide analog, and said identifying the type of nucleotide analog added may be repeated, either after repeating the blending with the extended oligonucleotide primer or after determining the amounts of each type of dideoxynucleotide or dideoxynucleotide analog remaining in the extension solution as the new first amount. As a result, the nucleotide at the active site of the target nucleic acid molecule is determined. Also disclosed is an apparatus and composition for carrying out this method.

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