专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

JP2009271087A Detection of the receptor oligomerization 审中-公开
标题翻译：检测受体低聚
公开(公告)号：JP2009271087A
公开(公告)日：2009-11-19
申请号：JP2009188699
申请日：2009-08-17
申请人： Aclara Biosciences Inc , アクララバイオサイエンシーズ, インコーポレイテッド
发明人： CHAN-HUI PO-YING , SHI YINING , PIDAPARTHI SAILAJA , DUA RAJIV , SINGH SHARAT
IPC分类号： G01N33/53 , C12Q1/68 , G01N20060101 , G01N27/447 , G01N33/483 , G01N33/542 , G01N33/543 , G01N33/556 , G01N33/566 , G01N33/567 , G01N33/68
CPC分类号： G01N33/566 , G01N33/542 , G01N2333/71 , G01N2333/726
摘要： PROBLEM TO BE SOLVED: To provide a method of detecting formation of oligomer complexes of molecules on surfaces of cell membranes. SOLUTION: This method employs pairs of tagged probes and cleaving probes, each of which binds specifically to a cell surface molecule. The tagged probe includes a molecular tag that is linked to a first binding compound through a cleavable linkage, and the cleaving probe includes a second binding agent and a cleavage-inducing moiety that can cleave the linkage when within a defined proximity thereto. Binding of two probes to cell surface molecules that have formed an oligomeric complex results in release of the molecular tag from the binding compound, and providing measure of formation of the complex. COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供检测细胞膜表面上分子的低聚物复合物的形成的方法。解决方案：该方法采用成对的标记探针和切割探针，每个探针和切割探针都与细胞表面分子特异性结合。标记的探针包括通过可切割键连接到第一结合化合物的分子标签，并且所述切割探针包括第二结合剂和切割诱导部分，其在限定的接近于其内时可切割连接。两个探针与形成低聚复合物的细胞表面分子的结合导致分子标签从结合化合物的释放，并提供复合物形成的测量。版权所有（C）2010，JPO＆INPIT

2. 发明专利

JP2004117376A Tag library compound, composition, kit, and method of using the same 审中-公开
标题翻译： TAG图书馆化合物，组合物，工具包及其使用方法
公开(公告)号：JP2004117376A
公开(公告)日：2004-04-15
申请号：JP2004001495
申请日：2004-01-06
申请人： Aclara Biosciences Inc , アクララバイオサイエンシーズ, インコーポレイテッド
发明人： SINGH SHARAT , MATRAY TRACY , SALIMI-MOOSAVI HOSSEIN
IPC分类号： C12N15/09 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C12Q1/68 , G01N21/76 , G01N21/78 , G01N27/447 , G01N33/483 , G01N33/53 , G01N33/532 , G01N33/533 , G01N33/58 , G01N33/68
CPC分类号： C40B50/16 , C07H19/06 , C07H19/10 , C07H21/00 , C40B20/08 , C40B40/08 , C40B70/00
摘要： PROBLEM TO BE SOLVED: To provide a separable composition in use for detecting multiplex assays. SOLUTION: The composition has a region which works as a connection region, where an identifying Tag (called an eTag TM reporter) in use for electrokinetic analysis can be cut off, a mass alternation region, a charge alternation region and a detectable region. The number of different regions partially depends on the method of separation and identification. A compound having these separate regions finds out the way of usage in relation to other compounds where these regions are combined in the same area. COPYRIGHT: (C)2004,JPO
摘要翻译：要解决的问题：提供用于检测多重测定的可分离组合物。组合物具有作为连接区域的区域，其中可以切断用于电动分析的识别标签（称为eTag TM 报告人），质量交替区域，电荷交替区域和可检测区域。不同地区的数量部分取决于分离和鉴定的方法。具有这些分开的区域的化合物找到与其中这些区域在相同区域中组合的其它化合物的使用方式。版权所有（C）2004，JPO

3. 发明申请

WO03042398A2 MULTIPLEXED ANALYSIS BY CHROMATOGRAPHIC SEPARATION OF MOLECULAR TAGS 审中-公开
标题翻译：通过分子标记的色谱分离进行多重分析
公开(公告)号：WO03042398A2
公开(公告)日：2003-05-22
申请号：PCT/US0235864
申请日：2002-11-08
申请人： ACLARA BIOSCIENCES INC
发明人： CHENNA AHMED , MATRAY TRACY , HERNANDEZ VINCENT , HOOPER HERBERT , SINGH SHARAT
IPC分类号： C12Q1/68 , G01N30/02 , C12Q
CPC分类号： C12Q1/6809 , G01N2030/027 , C12Q2565/102 , C12Q2565/137
摘要： Methods and kits are disclosed for determining, either in a homogeneous or heterogeneous assay format, one or more target analytes in a sample using binding compositions coupled to molecular tags by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such binding compositions under conditions that permit stable complexes to form between the binding compositons and analytes. In one aspect of the invention, the interaction between the binding compositions and their respective binding sites brings a cleavage-inducing moiety into close proximity to cleavable linkages or provides a recognizable substrate for a cleavage-inducing moiety. In this way, one or more molecular tags for each of the analytes are released from the complexes. Released molecular tags are chromatographically separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.
摘要翻译：公开了用于以同质或非均相测定形式测定样品中一种或多种目标分析物的方法和试剂盒，其使用通过可切割键连接分子标签的结合组合物。通常，在允许在结合组合物和分析物之间形成稳定的复合物的条件下，形成包含样品和包含多种这样的结合组合物的试剂的测定混合物。在本发明的一个方面，结合组合物和它们各自的结合位点之间的相互作用使切割诱导部分靠近可裂解连接，或为切割诱导部分提供可识别的底物。以这种方式，每个分析物的一个或多个分子标签从配合物中释放出来。释放的分子标签被色谱分离，并且基于释放和分离的分子标签的分析确定靶分析物的存在和/或量。

4. 发明申请

WO0056925A3 METHODS FOR SINGLE NUCLEOTIDE POLYMORPHISM DETECTION 审中-公开
标题翻译：单核苷酸多态性检测方法
公开(公告)号：WO0056925A3
公开(公告)日：2002-04-11
申请号：PCT/US0006135
申请日：2000-03-08
申请人： ACLARA BIOSCIENCES INC
发明人： SINGH SHARAT , ULLMAN EDWIN F
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6869 , C12Q2565/30 , C12Q2565/125 , C12Q2563/149 , C12Q2523/319 , C12Q2523/107
摘要： Methods and compositions are provided for determining large numbers of single nucleotide polymorphisms in target DNA employing particles having (1) primers complementary to sequences in the target DNA where the next succeeding 3'-nucleotide is a potential single nucleotide polymorphism and coding composition members, where the members are unique for each primer, and (2) differentially labeled terminating nucleotides, where the label permits separation of the terminating nucleotides. Desirably the particles are separated into groups having a common prevalent next succeeding nucleotide. The particles and target DNA are combined under nucleotide extending conditions, the particles separated into groups in accordance with the terminating nucleotide and the coding members identified, so that one knows the sequence and the single nucleotide polymorophism. Various protocols are provided for the determination.
摘要翻译：提供了用于使用具有（1）与目标DNA中的序列互补的引物，其中下一个后续的3'-核苷酸是潜在的单核苷酸多态性和编码组成成员的粒子来确定靶DNA中大量单核苷酸多态性的方法和组合物，其中成员对于每个引物是独特的，和（2）差异标记的终止核苷酸，其中标记允许分离终止的核苷酸。理想地，将颗粒分离成具有普通的下一个后续核苷酸的基团。颗粒和靶DNA在核苷酸延伸条件下合并，根据终止的核苷酸和鉴定的编码成员将颗粒分成组，以便人们知道序列和单核苷酸多形性。提供了各种协议用于确定。

5. 发明公开

EP1180112A4 DETECTION USING DEGRADATION OF A TAGGED SEQUENCE 审中-公开
标题翻译：证明通过去除显着的序列的
公开(公告)号：EP1180112A4
公开(公告)日：2004-06-23
申请号：EP00926444
申请日：2000-04-28
申请人： ACLARA BIOSCIENCES INC
发明人： SINGH SHARAT
IPC分类号： C12N15/09 , C07B61/00 , C07H19/06 , C07H19/10 , C07H21/00 , C12Q1/68 , C07H21/02
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C40B70/00 , G01N33/561 , Y10S435/81
摘要： Methods and compositions are provided for detecting DNA sequences, particularly single nucleotide polymorphisms, using a pair of nucleotide sequences, a primer and a snp detection sequence, where the snp detection sequence binds downstream from the primer to the target DNA in the direction of primer extension. The snp detection sequence is characterized by having a nucleotide complementary to the snp and adjacent nucleotides complementary to adjacent nucleotides in the target and an electrophoretic tag bonded to the 5'-nucleotide. The pair of sequences is combined with the target DNA under primer extension conditions, where the polymerase has 5'-3' exonuclease activity. When the snp is present, the electrophoretic tag is released and can be detected by electrophoresis as indicative of the presence of the snp in the target DNA. The sequence containing the snp is exemplary of DNA sequences of interest generally.

6. 发明公开

EP1581796A4 MULTIPLEX ANALYTICAL PLATFORM USING MOLECULAR TAGS 审中-公开
标题翻译：随着分子标签多重分析平台
公开(公告)号：EP1581796A4
公开(公告)日：2008-02-13
申请号：EP03815205
申请日：2003-12-12
申请人： ACLARA BIOSCIENCES INC
发明人： MATRAY TRACY , SINGH SHARAT , MACEVICZ STEPHEN C
IPC分类号： C12P19/34 , C12Q1/68 , G01N20060101 , G01N1/00
CPC分类号： C12Q1/6811 , C12Q2563/167
摘要： Compositions and methods are disclosed for detecting multiple target analytes, particularly polynucleotide target analytes. In accordance with one aspect of the invention, a template-dependent extension reaction is performed to generate detection probes, such that each detection probe has (i) at least one molecular tag attached by a cleavable linkage and (ii) either a capture moiety or a cleavage-inducing moiety attached. The template-dependent extension reaction may be carried out directly on a polynucleotide analyte to generate molecular tags, wherein the polynucleotide analyte serves as a template in the template-dependent extension reaction, or it may be carried out indirectly on an oligonucleotide label that, in turn, is attached to a binding moiety specific for an analyte of interest. In either case, a plurality of molecular tags are generated, after which they are separated and identified to determine the presence or absence or the quantity of the target analytes in a sample.

7. 发明申请

WO2004087887A2 INTRACELLULAR COMPLEXES AS BIOMARKERS 审中-公开
标题翻译：细胞内复合物作为生物标志物
公开(公告)号：WO2004087887A2
公开(公告)日：2004-10-14
申请号：PCT/US2004009805
申请日：2004-03-30
申请人： ACLARA BIOSCIENCES INC , SINGH SHARAT , BADAL M YOUSSOUF , JIN XUENGUANG , SALIMI-MOOSAVI HOSSEIN
发明人： SINGH SHARAT , BADAL M YOUSSOUF , JIN XUENGUANG , SALIMI-MOOSAVI HOSSEIN
IPC分类号： A61B20060101 , C07K16/28 , C12N20060101 , C12Q3/00 , G01N33/542 , G01N33/543 , G01N33/574 , C12N
CPC分类号： G01N33/57492 , C07K16/2863 , G01N33/542 , G01N2333/485 , G01N2800/52
摘要： The invention provides a method for determining a disease status of a patient by measuring expression levels of selected intracellular complexes. In one aspect of the invention, the activation status of apoptotic pathways in a patient sample is determined by measuring relative amounts of protein-protein complexes that are characteristic of the apoptotic pathways. In particular, the invention provides a method of determining the activation status of the mitochondrial apoptotic pathway by simultaneously measuring relative amounts of complexes between 14-3-3 proteins and BAD proteins on the one hand and complexes of Bcl-2 proteins and BAD proteins on the other hand. Preferably, methods of the invention are implemented by using sets of binding compounds having releasable molecular tags that are specific for multiple components of one or more complexes formed within apoptotic pathways. After binding molecular tags are released and separated from the assay mixture for analysis.
摘要翻译：本发明提供了一种通过测量所选细胞内复合物的表达水平来确定患者的疾病状态的方法。在本发明的一个方面，通过测量凋亡通路特征的蛋白质 - 蛋白质复合物的相对量来确定患者样品中凋亡途径的活化状态。特别地，本发明提供了一种通过同时测量14-3-3蛋白和BAD蛋白之间的复合物的相对量以及Bcl-2蛋白和BAD蛋白的复合物的相对量来确定线粒体凋亡途径的激活状态的方法另一方面。优选地，本发明的方法通过使用具有可释放分子标签的结合化合物组来实现，所述可释放分子标签对在凋亡途径内形成的一种或多种复合物的多种组分是特异性的。结合分子标签后，从测定混合物中分离出分子标签进行分析。

8. 发明申请

WO03006947A3 CELL-SCREENING ASSAY AND COMPOSITION 审中-公开
标题翻译：细胞筛选测定和组成
公开(公告)号：WO03006947A3
公开(公告)日：2003-05-08
申请号：PCT/US0221339
申请日：2002-07-05
申请人： ACLARA BIOSCIENCES INC , SINGH SHARAT , CHAN-HUI PO-YING
发明人： SINGH SHARAT , CHAN-HUI PO-YING
IPC分类号： G01N33/50 , G01N33/58 , C12Q1/66 , C07H21/02 , C07H21/04 , C12N9/12 , C12P21/06 , G01N27/26
CPC分类号： G01N33/5023 , G01N33/5008 , G01N33/581
摘要： The present invention discloses methods for multiplexed cellular assays. The methods can be used for a simultaneous quantitation of transcription levels from multiple promoters, multiple drugs, or for monitoring effects on multiple protein-protein interactions. These methods make use of reporter gene constructs that couple a single reporter enzyme coding sequence with one or more promoter regions, and a set of probes that are subject to degradation by the reporter enzyme, producing a set of reporters that can be separated from each other by differences in their mobilities. Multiplexed assays are achieved by combining a set of cell populations, wherein each population contains a distinct reporter gene construct and a distinct probe. Various treatments are then carried out on the mixture of cells. Treatments that induce transcription from specific promoters will cause synthesis of the reporter enzyme in that particular cell population, leading to degradation of the corresponding probe. The generation of specific reporters is determined by electrophoresis, or other means of measuring mobility, and is correlated with an effect of the treatment on transcription from a defined promoter.
摘要翻译：本发明公开了用于多路复用细胞分析的方法。该方法可用于同时定量多种启动子，多种药物的转录水平，或用于监测对多种蛋白质 - 蛋白质相互作用的影响。这些方法利用将单个报道酶编码序列与一个或多个启动子区偶联的报道基因构建体和一组受到报道酶降解的探针，产生一组可以彼此分离的报道分子通过其流动性的差异。通过组合一组细胞群实现复合测定，其中每个群包含独特的报道基因构建体和独特的探针。然后对细胞混合物进行各种处理。诱导特定启动子转录的处理将引起该特定细胞群中报道酶的合成，导致相应探针的降解。特定报道分子的产生通过电泳或其他测量流动性的手段来确定，并与治疗对来自确定的启动子的转录的影响相关。

9. 发明申请

WO0173113A2 MONOOXYGENASE ASSAYS 审中-公开
标题翻译： MONOOXYGENASE测试
公开(公告)号：WO0173113A2
公开(公告)日：2001-10-04
申请号：PCT/US0110155
申请日：2001-03-29
申请人： ACLARA BIOSCIENCES INC
发明人： SINGH SHARAT , CHENNA AHMED , SALIMI-MOOSARI HOSSEIN , GIBBONS IAN
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26 , G01N2333/795 , G01N2333/80 , G01N2333/90245 , Y10S435/968
摘要： Cytochrome P-450 assay methods and kits for the methods are provided employing a cytochrome P-450 enzyme, substrates characterized by having an oxidizable methylene group oxidized to an aldehyde and a fluorescent hydrazine. A fluorescent hydrazine is added to the reaction mixture and the resulting hydrazone analyzed by capillary electrophoresis. The method finds use in evaluating compounds for enzyme modulating activity.
摘要翻译：使用细胞色素P-450酶提供细胞色素P-450测定方法和用于该方法的试剂盒，其特征在于具有氧化成醛的可氧化亚甲基基团和荧光肼。向反应混合物中加入荧光肼，通过毛细管电泳分析得到的腙。该方法用于评价化合物的酶调节活性。

10. 发明申请

WO0067907A3 SAMPLE EVAPORATIVE CONTROL 审中-公开
标题翻译：样品蒸发控制
公开(公告)号：WO0067907A3
公开(公告)日：2001-02-08
申请号：PCT/US0012826
申请日：2000-05-10
申请人： ACLARA BIOSCIENCES INC
发明人： SINGH SHARAT , ULLMAN EDWIN , GIBBONS IAN , BOONE TRAVIS , XIAO VIVIAN , BJORNSON TORLIEF , HOOPER HERBERT
IPC分类号： G01N27/447 , B01D57/02 , B01J19/00 , B01J19/16 , B01L3/00 , B01L7/00 , B03C5/00 , C12M1/00 , C12Q1/00 , C40B60/14 , G01N1/34 , G01N35/00 , G01N35/08 , G01N37/00
CPC分类号： B01L3/5027 , B01F13/0059 , B01J19/16 , B01J2219/00317 , B01L3/5025 , B01L3/502723 , B01L2200/0642 , B01L2200/142 , B01L2300/0861 , B01L2400/0406 , B01L2400/0415 , B01L2400/0688 , C40B60/14 , G01N1/34 , G01N2035/00237 , G01N2035/00287
摘要： Devices and methods are provided using microfluidic devices for manipulating small volumes and determining a variety of chemical and physical events. The devices rely upon an opening to the atmosphere of a small volume in a zone, where a sample is placed in the zone where evaporation can occur. The zone is maintained in contact with a liquid medium that serves to replenish the liquid in the zone and maintain the composition of the mixture in the zone substantially constant. The diffusion of components in the zone is restricted during the course of the determination by the liquid flux into the zone.
摘要翻译：使用微流体装置提供装置和方法来操纵小体积并确定各种化学和物理事件。这些装置依赖于在区域中的小体积的大气的开口，其中样品被放置在可能发生蒸发的区域中。该区域保持与液体介质接触，液体介质用于补充该区域中的液体，并将该混合物的组成保持在该区域中基本上恒定。在通过进入该区域的液体通量的确定过程中，区域中的组分的扩散受到限制。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式