专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1994020636A1 GENETICALLY ENGINEERED ENZYMES AND THEIR CONJUGATES FOR DIAGNOSTIC ASSAYS 审中-公开
标题翻译：基因工程酶及其与诊断学结合的诊断
公开(公告)号：WO1994020636A1
公开(公告)日：1994-09-15
申请号：PCT/US1994002539
申请日：1994-03-09
申请人： ABBOTT LABORATORIES
发明人： ABBOTT LABORATORIES , BRATE, Elaine, M. , BRENNAN, Catherine, A. , BRIDON, Dominique, P. , JAFFE, Keeve, D. , KRAFFT, Grant, A. , MANDECKI, Wlodzimierz , MARCH, Steven, C. , RUSSELL, John, C. , YUE, Vincent, T.
IPC分类号： C12Q01/00
CPC分类号： C07K14/59 , C07K2319/40 , C12N9/16 , C12Q1/42
摘要： This invention relates to genetically engineered enzymes, their ligand conjugates, their manufacture, and their use in qualitative or quantitative assays. A hybrid enzyme, such as an AP-epitope, has a foreign amino acid moiety (an epitope) inserted near the active site of the starting AP enzyme. The foreign amino acid moiety binds with an analyte, and, as a consequence of this binding, the enzymatic activity of the hybrid enzyme, AP-epitope, is modified. The changes in the enzymatic activity are dependent upon the presence, or the amount, of the analyte. In another embodiment, the hybrid enzyme consists of a cysteine introduced near the active site of an AP to give a hybrid enzyme. The cysteine on the hybrid enzyme serves as a point of conjugation of a ligand, such as theophylline, ferritin, thyroxine, or digoxigenin, to form the hybrid enzyme-ligand conjugate. The ligand binds with an antibody, an analyte or a binding molecule to an analyte and as a result of this binding, the enzymatic activity of the hybrid enzyme-ligand conjugate is modified or modulated.
摘要翻译：本发明涉及基因工程改造的酶，其配体缀合物，其制备及其在定性或定量测定中的用途。诸如AP-表位的杂交酶在起始AP酶的活性位点附近插入外源氨基酸部分（表位）。外来氨基酸部分与分析物结合，并且作为该结合的结果，修饰了杂合酶AP-表位的酶活性。酶活性的变化取决于分析物的存在或数量。在另一个实施方案中，杂交酶由在AP的活性位点附近引入的半胱氨酸组成以产生杂交酶。杂交酶上的半胱氨酸用作配体如茶碱，铁蛋白，甲状腺素或地高辛的缀合点，以形成杂交酶 - 配体缀合物。配体与分析物结合抗体，分析物或结合分子，作为该结合的结果，修饰或调节杂交酶 - 配体结合物的酶活性。

2. 发明公开

EP0688364A1 GENETICALLY ENGINEERED ENZYMES AND THEIR CONJUGATES FOR DIAGNOSTIC ASSAYS 失效
标题翻译：基因工程酶及其偶联物诊断测试
公开(公告)号：EP0688364A1
公开(公告)日：1995-12-27
申请号：EP94913281.0
申请日：1994-03-09
申请人： ABBOTT LABORATORIES
发明人： BRATE, Elaine, M. , BRENNAN, Catherine, A. , BRIDON, Dominique, P. , JAFFE, Keeve, D. , KRAFFT, Grant, A. , MANDECKI, Wlodzimierz , MARCH, Steven, C. , RUSSELL, John, C. , YUE, Vincent, T.
IPC分类号： G01N33 , C07K14 , C12N9 , C12N15 , C12Q1 , C12R1
CPC分类号： C07K14/59 , C07K2319/40 , C12N9/16 , C12Q1/42
摘要： This invention relates to genetically engineered enzymes, their ligand conjugates, their manufacture, and their use in qualitative or quantitative assays. A hybrid enzyme, such as an AP-epitope, has a foreign amino acid moiety (an epitope) inserted near the active site of the starting AP enzyme. The foreign amino acid moiety binds with an analyte, and, as a consequence of this binding, the enzymatic activity of the hybrid enzyme, AP-epitope, is modified. The changes in the enzymatic activity are dependent upon the presence, or the amount, of the analyte. In another embodiment, the hybrid enzyme consists of a cysteine introduced near the active site of an AP to give a hybrid enzyme. The cysteine on the hybrid enzyme serves as a point of conjugation of a ligand, such as theophylline, ferritin, thyroxine, or digoxigenin, to form the hybrid enzyme-ligand conjugate. The ligand binds with an antibody, an analyte or a binding molecule to an analyte and as a result of this binding, the enzymatic activity of the hybrid enzyme-ligand conjugate is modified or modulated.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式