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    • 10. 发明申请
    • ASSAY DEVICES AND METHOD OF DETECTING A TARGET ANALYTE
    • 测定装置和检测目标分析仪的方法
    • WO2015191419A3
    • 2016-04-21
    • PCT/US2015034624
    • 2015-06-08
    • 3M INNOVATIVE PROPERTIES CO
    • ROSCOE STEPHEN BKANG MYUNGCHANBRUTINEL EVAN DMILLER JESSE DCLINE RENEE KDAI MINGHUALIU HSI-CHOU
    • B01L3/00
    • B01L3/5029B01L3/502B01L2200/16B01L2300/0825B01L2400/0406B01L2400/0478G01N33/5304
    • Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.
    • 提供测定装置,包括具有样品入口的容器; 设置在所述容器内的柱塞; 至少一种试剂; 膜连接到柱塞的第一表面; 提供毛细管力的灯芯; 和检测区。 膜包括其中设置亲和成分的靶共轭区。 捕获化合物固定在位于靶共轭区和芯之间的检测区中。 还提供了检测目标分析物的方法,包括提供测定装置; 提供怀疑含有目标分析物的样品; 将样品添加到设备上; 允许样品沿着膜移动直到样品到达检测区域; 通过靶分析物与捕获化合物的反应固定目标分析物; 使固定化的目标分析物与试剂反应以产生可检测的信号; 并检测所产生的信号。