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    • 2. 发明公开
    • 알렉산드리움 타마렌스의 고체 배양 방법
    • 亚历山大藻固体培养方法
    • KR1020130070494A
    • 2013-06-27
    • KR1020120020795
    • 2012-02-29
    • 한양대학교 산학협력단
    • 진언선김시욱김재호김귀다정슬기
    • C12N1/12C12N11/04C12Q1/02
    • PURPOSE: A solid culture method of alexandrium tamarense is provided to ensure easy long-term storage of cells, to easily isolate pure single cell, and to study alexandrium tamarense. CONSTITUTION: A solid culture method of alexandrium tamarense comprises: a step of sterilizing a liquid medium containing a medium solidifier by pressure at high temperature; a step of cooling the medium, and mixing cells of alexandrium tamarense before the medium is solidified; and a step of solidifying the medium and culturing alexandrium tamarense. The medium solidifier contains a mixture of one or more selected from the group consisting of agar, serum, gelatin, silica gel, and albumin. The liquid medium is F2 medium.
    • 目的:提供塔玛亚历山大藻固体培养方法,确保细胞长期储存,容易分离纯单细胞,研究塔玛亚历山大藻。 构成:泰山A氏固体培养方法包括:在高温下通过压力对含有中等凝固剂的液体培养基进行灭菌的步骤; 在培养基固化之前冷却培养基的步骤,并混合塔尼玛白葡萄酒的细胞; 并培养培养基并培养塔玛亚历山大藻的步骤。 中等凝固剂含有选自琼脂,血清,明胶,硅胶和白蛋白中的一种或多种的混合物。 液体介质为F2介质。
    • 5. 发明公开
    • 수용성 유기점토를 이용한 유해조류 제어방법
    • 使用有机粘土控制有害的阿尔法的方法
    • KR1020120025252A
    • 2012-03-15
    • KR1020100087536
    • 2010-09-07
    • 조선대학교산학협력단한양대학교 산학협력단
    • 김시욱신현재진언선김연미
    • A01N61/02C01B33/40A01N59/00A01P13/00
    • A01N59/06C01B33/40
    • PURPOSE: A method for controlling harmful algae using water soluble organic clay is provided to prevent effect to other marine ecosystems and to selectively prevent the harmful algae. CONSTITUTION: A water soluble organic clay controls harmful algae. The water soluble organic clay is metal ionic phylloxilicate having attached functional group selected from amino propyl, mercaptopropyl, methyl, phenyl, methacryloxy propyl, and glycyldeoxypropyl group. The functional group is amino propyl. A method for preparing the water soluble organic clay comprises: a step of dissolving magnesium chloride hexahydrite in ethanol; a step of adding 2-aminopropyl triethoxy silane(APTES) to the magnesium chloride hexahydrite to form a white float; a step of stirring the solution having the white float; a step of centrifuging the solution to separate into precipitate and supernatant; a step of suspending the precipitate in ethanol and centrifuging 2-5 times; and a step of dissolving water soluble organic clay in distilled water.
    • 目的:提供一种使用水溶性有机粘土控制有害藻类的方法,以防止对其他海洋生态系统的影响,并有选择地防治有害藻类。 构成:水溶性有机粘土控制有害藻类。 水溶性有机粘土是具有选自氨基丙基,巯基丙基,甲基,苯基,甲基丙烯酰氧基丙基和甘氨酰基脱氧丙基的官能团的金属离子型硅酸盐。 官能团为氨基丙基。 制备水溶性有机粘土的方法包括:将六水合氯化镁在乙醇中的步骤; 向氯化镁六水合物中加入2-氨基丙基三乙氧基硅烷(APTES)以形成白色浮渣的步骤; 搅拌具有白色浮液的溶液的步骤; 将溶液离心分离成沉淀物和上清液的步骤; 将沉淀物悬浮在乙醇中并离心2-5次的步骤; 以及将水溶性有机粘土溶解在蒸馏水中的步骤。