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    • 1. 发明授权
    • 표면장력으로 제어되는 미세유체소자
    • 由表面张力控制的微流体装置
    • KR100471377B1
    • 2005-03-11
    • KR1020020072344
    • 2002-11-20
    • 한국전자통신연구원
    • 정광효고종수윤현철양해식표현봉김성진신용범김윤태
    • C12Q1/00
    • 본 발명은 표면장력으로 제어되는 미세유체소자에 관한 것으로, 상부 기판 및 하부 기판이 결합되어 유체를 제어하고 반응시키는 미세유체소자에 있어서, 하부 기판은, 제1 유체 및 제2 유체가 각각 저장되는 제1 저장 챔버 및 제2 저장 챔버와, 제1 저장 챔버 및 제2 저장 챔버와 연결되는 감지 챔버와, 제1 유체 또는 제2 유체의 이동을 정지시키는 제1 유동 정지부 및 제2 유동 정지부와, 제1 유체 또는 제2 유체의 이동 속도를 감소시키는 유동 지연부와, 반응이 완료된 제1 유체 또는 제2 유체가 폐기되는 폐기 챔버 및 유체가 이동되도록 유동 지연부와 폐기 챔버 사이를 연결하는 유로를 포함하고, 상부 기판은 감지 챔버 내의 생화학반응을 측정하는 감지부를 포함하며, 제1 유체가 모세관 힘에 의해 감지 챔버로 이동하여 1차 생화학 반응이 일어나고, 소정 시간 경과 후 제2 유체가 모세관 힘에 의해 감지 챔버로 유입되면 제1 유체의 교체 및 2 차 생화학반응이 일어나는 것을 특징으로 한다. 따라서, 추가적인 장치 및 전원공급이 필요 없게 되어, 장치의 소형화, 휴대화가 가능하고, 제조비를 낮춤과 동시에 제조 수율을 높일 수 있을 뿐만 아니라, 사용 시 고장이 거의 없는 효과가 있다.
    • 2. 发明公开
    • 미소유체 혼합을 위한 미소 유체제어 소자
    • 用于混合微流控的微流体装置
    • KR1020040039571A
    • 2004-05-12
    • KR1020020067683
    • 2002-11-02
    • 한국전자통신연구원
    • 김성진신용범양해식박세호이대식윤태환김규원김윤태
    • B01F5/06
    • PURPOSE: A microfluidic device applied to a biochip is provided to mix microfluid by disposing hot wires in a reaction chamber to form layers and heating or cooling stored fluids. CONSTITUTION: The microfluidic device for mixing microfluid comprises a first flow channel delivering a first fluid(106); a second flow channel delivering a second fluid(108); a reaction chamber connected to ends of the first and second flow channels and having hot wires(104) for heating the inside; and a part for stopping flow connected to end of the reaction chamber so as to prevent the first and second fluids from moving. When the first and second fluids meet in the reaction chamber and form layers, the first and second fluids are heated or cooled using the hot wires to generate the density difference, thereby mixing the first and second fluids by gravity.
    • 目的:提供应用于生物芯片的微流体装置,通过在反应室中设置热丝以形成层并加热或冷却储存的流体来混合微流体。 构成:用于混合微流体的微流体装置包括:输送第一流体(106)的第一流动通道; 输送第二流体(108)的第二流动通道; 反应室连接到第一和第二流动通道的端部,并具有用于加热内部的热丝(104) 以及用于停止与反应室的端部连通的流动的部件,以防止第一和第二流体移动。 当第一和第二流体在反应室中相交并形成层时,使用热丝将第一和第二流体加热或冷却,以产生密度差,从而通过重力混合第一和第二流体。
    • 3. 发明公开
    • 비정질 실리콘 박막 트랜지스터를 이용한 생체 분자 감지를 위한 형광 검출 소자
    • 用于使用非晶硅薄膜晶体管检测生物分子的荧光检测装置和使用其检测荧光的方法
    • KR1020040035104A
    • 2004-04-29
    • KR1020020063838
    • 2002-10-18
    • 한국전자통신연구원
    • 윤태환양해식이대식박세호신용범김규원김성진김윤태
    • C12Q1/68
    • PURPOSE: A fluorescence detecting device for detecting biomolecule using amorphous-silicon thin film transistor and a method for detecting the fluorescence using the same are provided, thereby reducing the size and costs of DNA chip because optical devices are not used, and requiring no fluorescence stimulating light blocking filter by using a transparent substrate. CONSTITUTION: A fluorescence detecting device for detecting biomolecule using amorphous-silicon thin film transistor comprises a first substrate with a probe biomolecule; a transparent substrate(102) having a first domain(I), a second domain(II) and a third domain(III), and having a first surface(102a) where a light is irradiated and a second surface(102b) arranged to face the first substrate; a light thin film transistor(TFToptic) producing electric charge responding to the fluorescence produced from the biomolecule on the second surface(102b) and positioned in the first domain(I); a capacity(CAP) storing the electric charge produced from the light thin film transistor(TFToptic) and positioned in parallel to the light thin film transistor(TFToptic) in the second domain(II); and a transfer thin film transistor(TFTtrans) transferring the electric charge to an additional analysis system and positioned in parallel to the capacity(CAP) in the third domain(III).
    • 目的:提供一种用于使用非晶硅薄膜晶体管检测生物分子的荧光检测装置和使用其的荧光检测方法,从而由于不使用光学器件而不需要荧光刺激而降低DNA芯片的尺寸和成本 遮光过滤器采用透明基板。 构成:使用非晶硅薄膜晶体管检测生物分子的荧光检测装置,包括具有探针生物分子的第一基底; 具有第一结构域(I),第二结构域(II)和第三结构域(III)的透明基板(102),并且具有照射光的第一表面(102a)和第二表面(102b) 面对第一衬底; 产生电荷的光薄膜晶体管(TFToptic),产生响应于由第二表面(102b)上的生物分子产生并位于第一域(I)中的荧光的电荷; 存储从所述光薄膜晶体管(TFT光电)产生并与所述第二域(II)中的所述薄膜晶体管(TFToptic)并行定位的电荷的容量(CAP); 和转移薄膜晶体管(TFTtrans),其将电荷转移到另外的分析系统并且与第三域(III)中的容量(CAP)平行放置。
    • 4. 发明公开
    • 표면 플라즈몬 공명 센서 시스템
    • 表面质谱共振传感器系统
    • KR1020030047567A
    • 2003-06-18
    • KR1020010078270
    • 2001-12-11
    • 한국전자통신연구원
    • 표현봉신용범정지욱김민곤이상경신동호박선희
    • G01B11/00
    • G01N21/553
    • PURPOSE: A surface plasmon resonance sensor system is provided to prevent a metal layer from being deformed by forming a transparent thin film on the metal layer and bonding the transparent thin film to the metal layer by using an adhesive layer. CONSTITUTION: A surface plasmon resonance sensor system includes a transparent substrate(13a). A first adhesive layer, a conductive thin film(13b), a second adhesive layer(13d), and a transparent layer(13e) are sequentially deposited on the transparent substrate(13a), thereby forming a sensor section of a sensor chip(13). A sample to be measured is positioned at an upper portion of the sensor chip(13). A prism(12) is attached to a lower portion of the sensor chip(13) in order to create a plasmon resonance by reacting with a surface of the conductive thin film(13b). A light source(11) is provided to supply light to the sensor chip(13) through the prism(12). A light receiving section(14) receives light reflected from the sensor chip(13).
    • 目的:提供表面等离子体共振传感器系统,以通过在金属层上形成透明薄膜并通过使用粘合剂层将透明薄膜粘合到金属层来防止金属层变形。 构成:表面等离子体共振传感器系统包括透明基板(13a)。 顺序地在透明基板(13a)上淀积第一粘合层,导电薄膜(13b),第二粘合剂层(13d)和透明层(13e),从而形成传感器芯片(13)的传感器部分 )。 要测量的样品位于传感器芯片(13)的上部。 棱镜(12)安装在传感器芯片(13)的下部,以便通过与导电薄膜(13b)的表面反应而产生等离子体共振。 提供光源(11)以通过棱镜(12)向传感器芯片(13)提供光。 光接收部分(14)接收从传感器芯片(13)反射的光。
    • 7. 发明公开
    • 저전력형 미세 열순환 소자 및 그 제조 방법
    • 低功耗微型热循环器及其制造方法
    • KR1020040046175A
    • 2004-06-05
    • KR1020020074014
    • 2002-11-26
    • 한국전자통신연구원
    • 이대식양해식고종수윤태환김효겸신용범김성진박세호김규원김윤태
    • C12M1/00G01N27/26
    • PURPOSE: A low power consumption microfabricated thermal cycler and a method for fabrication of the same are provided, thereby accurately and minutely controlling the temperature of the reaction occurring area, so that it can be applied to various bio chips including PCR chip, protein chip, DNA chip, drug delivery system, micro biological/chemical reactor and lab-on-a-chip. CONSTITUTION: The low power consumption microfabricated thermal cycler comprises an upper board(112) and a lower board(100), wherein the upper board(112) comprises an fluid inlet(114) and outlet, a reaction chamber(118), and a fluid channel(116) connecting the inlet(114) and outlet to the reaction chamber(118); and the lower board(100) comprises an insulated heating thin layer(106) formed on the lower board(100), a heating means(102) formed on the insulated heating thin layer(106), a temperature sensor(104) formed on the insulated heating thin layer(106), and an insulating layer(108) covering the heating means(102) and the temperature sensor(104); and the insulated heating thin layer(106) is composed of Si3N4, SiO2, Si3N4/SiO2/Si3N4 or SiO2/Si3N4/SiO2 and has thickness of 0.1 to 10 micrometer.
    • 目的:提供一种低功耗微加热热循环仪及其制造方法,从而准确,精确地控制反应发生区域的温度,从而可应用于各种生物芯片,包括PCR芯片,蛋白芯片, DNA芯片,药物输送系统,微生物/化学反应器和芯片实验室。 构成:低功耗微加热热循环仪包括上板(112)和下板(100),其中上板(112)包括流体入口(114)和出口,反应室(118)和 将入口(114)和出口连接到反应室(118)的流体通道(116); 并且所述下板(100)包括形成在所述下板(100)上的绝缘加热薄层(106),形成在所述绝缘加热薄层(106)上的加热装置(102),形成在所述绝缘加热薄层 绝缘加热薄层(106)和覆盖加热装置(102)和温度传感器(104)的绝缘层(108)。 绝缘加热薄层(106)由Si3N4,SiO2,Si3N4 / SiO2 / Si3N4或SiO2 / Si3N4 / SiO2组成,厚度为0.1〜10微米。
    • 8. 发明公开
    • 표면장력으로 제어되는 미세유체소자
    • 表面张力控制微流体装置
    • KR1020040043897A
    • 2004-05-27
    • KR1020020072344
    • 2002-11-20
    • 한국전자통신연구원
    • 정광효고종수윤현철양해식표현봉김성진신용범김윤태
    • C12Q1/00
    • PURPOSE: Provided is a surface tension-controlled microfluidic device which moves the fluid only through natural flowing due to the capillary attraction in a controlled manner, and is effectively used in bio chips requiring micro and precise flow control. CONSTITUTION: A surface tension-controlled microfluidic device comprises an upper substrate and a lower substrate(100) for controlling and reacting the fluid. The lower substrate comprises: first and second storage chambers(102,104) for storing first and second fluids, respectively; at least one sensor chamber(106) connected to the first and second storage chambers; first and second flow stoppers(112,114) for stopping the flows of the first and second fluids; a flow delayer(116) for reducing the flow speed of the first or second fluids; a waste chamber(120) for waste-disposing the reacted first or second fluid; and a fluid passage(118) for interconnecting the flow delayer and the waste chamber to flow the fluid. The upper substrate comprises at least one sensor for measuring the biochemical reaction in the sensor chamber. The first fluid moves to the sensor chamber due to the capillary attraction to make the primary biochemical reaction, and after a predetermined time passes by, the second fluid flows into the sensor chamber due to the capillary attraction to replace the first fluid while making the secondary biochemical reaction.
    • 目的:提供一种表面张力控制的微流体装置,其仅通过受到毛细管吸引的自然流动使流体移动,并且有效地用于需要微细和精确流动控制的生物芯片中。 构成:表面张力控制的微流体装置包括用于控制和使流体反应的上基板和下基板(100)。 下基板包括:用于分别存储第一和第二流体的第一和第二储存室(102,104) 连接到第一和第二储存室的至少一个传感器室(106) 用于停止第一和第二流体的流动的第一和第二止流器(112,114) 流量延迟器(116),用于降低第一或第二流体的流速; 用于废物处理所述反应的第一或第二流体的废物室(120); 以及用于将流动延迟器和废物室互连以使流体流动的流体通道(118)。 上基板包括用于测量传感器室中的生化反应的至少一个传感器。 第一流体由于毛细管吸引而移动到传感器室,以进行初级生化反应,并且在预定时间过去之后,由于毛细管吸引力,第二流体流入传感器室,以替代第一流体同时使次级 生化反应。
    • 9. 发明公开
    • 주기적 가열과 냉각을 통한 혼성화 방법 및 이를 사용하는폴리뉴클레오타이드 검출 장치
    • 使用周期性加热和冷却的混合方法和使用其的多核苷酸检测装置
    • KR1020040038445A
    • 2004-05-08
    • KR1020020067391
    • 2002-11-01
    • 한국전자통신연구원
    • 박세호양해식이대식신용범김규원윤태환김성진윤현철김윤태
    • C12Q1/68
    • PURPOSE: A hybridization method through periodic heating and cooling and a polynucleotide detection apparatus using the same are provided, thereby reducing the time for hybridization and increasing the specificity of hybridization, so that the disorder of target polynucleotide can be rapidly and accurately detected. CONSTITUTION: A hybridization method through periodic heating and cooling comprises the steps of: supplying a probe with a target polynucleotide; and circulating a high temperature hybridization process, wherein the hybridization is carried out at higher temperature than the melting point of the probe and target polynucleotide hybrid, and a low temperature hybridization process wherein the hybridization is carried out at lower temperature than the melting point of the probe and target polynucleotide hybrid two or more times, wherein the high temperature is at least 20 deg. C as higher as the melting point; and the low temperature is at least 5 deg. C as lower as the melting point.
    • 目的:提供通过周期性加热和冷却的杂交方法和使用其的多核苷酸检测装置,从而减少杂交时间并增加杂交的特异性,从而可以快速准确地检测靶多核苷酸的病症。 构成:通过周期性加热和冷却的杂交方法包括以下步骤:向探针提供靶多核苷酸; 并循环高温杂交方法,其中杂交在比探针和靶多核苷酸杂交体的熔点更高的温度下进行,以及低温杂交方法,其中杂交在低于 探针和靶多核苷酸杂交两次或更多次,其中高温至少为20度。 C高于熔点; 低温至少为5度。 C低于熔点。