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    • 9. 发明公开
    • 구제역바이러스 아시아 1형 항체를 검출하기 위한 진단방법
    • 用于检测禽流感病毒类型的病毒的诊断方法1
    • KR1020110031012A
    • 2011-03-24
    • KR1020090088714
    • 2009-09-18
    • 대한민국(농림축산식품부 농림축산검역본부장)주식회사 메디안디노스틱
    • 고영준이향심정혜영허은정박종현이광녕김수미권창희주후돈장병식
    • G01N33/68C12N15/09G01N33/563
    • PURPOSE: A diagnosis method for detecting food-and-mouth disease virus type asia 1 antibody is provided to quickly measure vaccine antibody titer. CONSTITUTION: An antibody for food-and-mouth disease virus type asia 1 is detected using a gene recombinant protein antigen of food-and-mouth disease virus type asia 1 and food-and-mouth disease virus type asia 1-specific monoclonal antibody. A diagnosis method for detecting food-and-mouth disease virus type asia 1 antibody comprises: a step of reacting serotype common antibody 70-17 of food-and-mouth disease virus type asia 1, type C, O, and A in 96-well microplate; a step of washing monoclonal antibodies which are not conjugated on the plate to remove; a step of reacting with a test serum sample; a step of washing test serum sample which is not conjugated; a step of reacting horseradish peroxidase-conjugated type asia 1-specific monoclonal antibody; a step washing monoclonal antibodies which is not conjugated to the plate; and a step of measuring absorbance.
    • 目的:提供一种用于检测食源性病毒亚型1抗体的诊断方法,以快速测定疫苗抗体效价。 构成:使用食品口蹄疫病毒亚型1和口蹄疫病毒型亚洲1型单克隆抗体的基因重组蛋白抗原检测食品口蹄疫病毒亚型1的抗体。 一种用于检测食源病病毒亚型1抗体的诊断方法包括:将食物口蹄疫病毒亚型1(C,O和A型)的血清型共同抗体70-17与96- 微孔板 洗涤未在板上结合以除去的单克隆抗体的步骤; 与测试血清样品反应的步骤; 洗涤不结合的血清样品的步骤; 辣根过氧化物酶偶联型亚洲1型单克隆抗体的反应步骤; 步骤洗涤未与板结合的单克隆抗体; 和测量吸光度的步骤。