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    • 1. 发明公开
    • 불용성 베타-1,3-글루칸을 분해하는 신규한 베타-글루카네이즈 및 그의 생산방법
    • 新型β-葡萄糖苷酶消化不溶性BET-1,3-GLUCAN及其方法
    • KR1020100034358A
    • 2010-04-01
    • KR1020080093446
    • 2008-09-23
    • 한국생명공학연구원네이처런스(주)
    • 최의성손여진이인영김미경박영일
    • C12N9/42C12N9/00C12Q1/68
    • C12N15/70C12N15/81
    • PURPOSE: A novel beta-glucanase is provided to ensure excellent hydrolysis activity to insoluble beta-glucan and produce soluble beta-glucan without chemical wastes. CONSTITUTION: A protein having an activity of beta-glucanase has an amino acid of sequence number 1 or 90% or more homology with the amino acid. A nucleic acid molecule encoding the beta-glucanase protein has nucleic acid of sequence number 2. The protein contains a beta-1,3-glucan binding domain. The protein is derived from Cellulomonas sp. A pBluescript KSII(+) vector contains the nucleic acid molecule encoding the beta-glucanase protein. The vector is a yeast expression vector and contains a promoter and secretion signal gene. The promoter is GAL10, GAPDH, PGK, ADH, PHO5, GAL1, SED1, MOX, TEF or TPI A gene encoding the secretion signal is MFα, PHO5, SUC2, AMY, SED or killer toxin. A transformant transformed by the vector is E. coli or Saccharomyces cerevisiae yeast. The beta-glucanase is obtained by culturing transformant and collecting beta-glucanase from culture.
    • 目的:提供一种新的β-葡聚糖酶,以确保对不溶性β-葡聚糖的优异的水解活性,并产生无化学废物的可溶性β-葡聚糖。 构成:具有β-葡聚糖酶活性的蛋白质具有与氨基酸序列1或90%以上同源性的氨基酸。 编码β-葡聚糖酶蛋白的核酸分子具有序列号2的核酸。蛋白质含有β-1,3-葡聚糖结合结构域。 蛋白质来源于纤维单胞菌属 pBluescript KSII(+)载体含有编码β-葡聚糖酶蛋白质的核酸分子。 载体是酵母表达载体,含有启动子和分泌信号基因。 启动子是GAL10,GAPDH,PGK,ADH,PHO5,GAL1,SED1,MOX,TEF或TPI编码分泌信号的基因是MFα,PHO5,SUC2,AMY,SED或杀伤毒素。 由载体转化的转化体是大肠杆菌或酿酒酵母(Saccharomyces cerevisiae)酵母。 通过培养转化体并从培养物中收集β-葡聚糖酶获得β-葡聚糖酶。
    • 5. 发明授权
    • 미생물 배양에 의한 커들란을 생산하는 방법
    • 微生物培养如何生产葡萄干
    • KR100316467B1
    • 2001-12-20
    • KR1019990014200
    • 1999-04-21
    • 한국생명공학연구원네이처런스(주)
    • 박영훈이인영이중헌김미경정준기
    • C12N1/20C12N1/00
    • PURPOSE: A method for producing curdlan by cultivating a microorganism is provided, thereby the curdlan can be cheaply and effectively produced in a higher yield. CONSTITUTION: The curdlan by cultivating a microorganism is produced by batch or fed-batch fermentation of Agrobacterium sp. in a growth medium containing carbon source, nitrogen source, phosphate and inorganic salt, wherein the hydrogen concentration of the medium is adjusted to 6.5 to 7.5 in order for the nitrogen source to be enough during a cell-growing phase; and the hydrogen concentration is adjusted to pH 5.0 to 5.5 during a curdlan producing phase after depletion of nitrogen sauce, in which the batch fermentation of Agrobacterium sp. is carried out under medium conditions of the initial concentration of carbon source of 100 to 160 g/l, the initial ammonium concentration as a nitrogen source of 0.5 to 2.5 g/l, the initial phosphate concentration is 0.5 to 4.5 g/l; and the fed-batch fermentation of Agrobacterium sp. is carried out under medium conditions of the initial concentration of carbon source of 80 to 140 g/l, and the initial phosphate concentration of 0.5 to 4.5 g/l, by adjusting pH of medium with ammonia water as a nitrogen source, and the supply of the nitrogen source is stopped and the concentration of carbon source is adjusted to 20 g/l or more when the curdlan production is initiated.
    • 10. 发明公开
    • 베타-1,3-글루칸을 고농도로 생산하는 신균주
    • beta -1.3葡萄糖生产微生物
    • KR1020030097174A
    • 2003-12-31
    • KR1020020034360
    • 2002-06-19
    • 네이처런스(주)
    • 김미경이인영
    • C12N1/20
    • PURPOSE: Provided is a β-1,3 glucan producing microorganism which is a mutant of an Agrobacterium sp. strain and produces the microbial polysaccharide, β-1,3 glucan, in high yields, thereby increasing the production yield of β-1,3 glucan. CONSTITUTION: β-1,3 glucan producing microorganism, Agrobacterium sp. R259 KCTC 10197BP is provided, wherein Agrobacterium sp. R259 KCTC 10197BP shows blue in a medium containing aniline blue; and Agrobacterium sp. R259 KCTC 10197BP is produced by culturing Agrobacterium sp. ATCC 31750 in a YP broth medium at pH 7.0 and 30 deg. C for 15 to 17 hours; centrifuging the cultured medium and removing the supernatant; adding a citrate buffer solution and MNTG (N-methyl-N'-nitro-N-nitrosoguanidine) to the cell pellet and reacting them at 30 deg. C and 140 rpm for 1 hour; spreading MNTG treated bacterium on a YP agar medium containing aniline blue and culturing it at 30 deg. C for 48 hours; selecting colonies showing blue; culturing the colonies in a broth medium at pH 7.0 and 30 deg. C for 3 days; and selecting one strain showing the excellent polysaccharide producing activity.
    • 目的:提供作为农杆菌属突变体的β-1,3葡聚糖产生微生物。 以高产率生产微生物多糖,β-1,3葡聚糖,从而提高β-1,3-葡聚糖的产量。 构成:产生β-1,3葡聚糖的微生物,农杆菌属 提供了R259KCTC 10197BP,其中农杆菌属 R259 KCTC 10197BP在含有苯胺蓝的培养基中显示蓝色; 和农杆菌属 R259 KCTC 10197BP是通过培养农杆菌 ATCC 31750在pH 7.0和30℃的YP肉汤培养基中。 C 15〜17小时; 离心培养基并除去上清液; 向细胞沉淀中加入柠檬酸盐缓冲溶液和MNTG(N-甲基-N'-硝基-N-亚硝基胍),并在30℃下反应。 C和140rpm 1小时; 将MNTG处理的细菌扩散到含有苯胺蓝的YP琼脂培养基上,并在30℃培养。 C 48小时; 选择显示蓝色的殖民地 在pH 7.0和30度的培养基培养基中培养菌落。 C 3天; 并选择显示优异的多糖生产活性的一种菌株。