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    • 3. 发明公开
    • 선발표지 유전자 제거용 형질전환 벡터
    • 用于消除选择标记基因的转化载体
    • KR1020090084014A
    • 2009-08-05
    • KR1020080009933
    • 2008-01-31
    • 한국생명공학연구원
    • 권석윤이우용박정무
    • C12N15/64C12N15/09C12N15/10
    • A vector containing a cassette for removing gene for selecting vector of plant transformation is provided to reduce price and time for evaluating harmfulness and safety and enable easy cloning. A cassette for removing gene for selecting vector of plant transformation comprises a Cre recombinase which is operatively linked to pBip-L promoter, argE(ornithine deacetylase) gene, and selection marker gene. The cassette comprises a sequence number 1(SEQ ID NO:1) or 2. A method for producing a transformed plant in which selection marker gene is removed comprises: a step of inserting a target gene into the vector for removing selection marker gene to produce recombinant plant expression vector; a step of transforming the recombinant plant expression vector in a plant; and a step of treating tunicamycin and N-acetyl phosphinothricin to select a transformant.
    • 提供含有用于去除用于选择植物转化载体的基因的盒的载体,以降低用于评价有害性和安全性的价格和时间,并使得易于克隆。 用于去除用于选择植物转化载体的基因的盒包含与pBip-L启动子,argE(鸟氨酸脱乙酰酶)基因和选择标记基因有效连接的Cre重组酶。 所述盒包含序列号1(SEQ ID NO:1)或2。一种转化植物的制备方法,其中除去选择标记基因包括:将靶基因插入载体中以除去选择标记基因以产生 重组植物表达载体; 在植物中转化重组植物表达载体的步骤; 以及治疗衣霉素和N-乙酰基草丁膦选择转化体的步骤。
    • 4. 发明公开
    • 옥수수의 전위성 인자를 이용한 유전자, 프로모터 또는인핸서 발굴용 형질전환용 벡터
    • 使用玉米AC / DS可转移元件的转化载体用于调查与提高生产力,抗病性或品质的植物相关的基因,促进剂或增强剂
    • KR1020050000843A
    • 2005-01-06
    • KR1020030041403
    • 2003-06-25
    • 한국생명공학연구원
    • 정원일박정무정종현김환묵문제선
    • C12N15/82
    • C12N15/8202
    • PURPOSE: Transformation vectors using maize Ac/Ds transposable element for investigating a gene, a promoter or an enhancer are provided, which vectors easily produce an enhancer line in comparison with the prior transposon tagging vectors, and are inserted into a larger number of single genomes to obtain numerous frame-free vectors, so that they can be useful for development of genes or promoters related to improvement of productivity, disease resistance or quality of plants. CONSTITUTION: The transformation vector(KCTC 10475BP) using maize Ac(activator(transposase))/Ds(dissociation) transposable element for investigating a gene, a promoter or an enhancer is provided, wherein the maize Ac/Ds transposable element has the nucleotide sequence set forth in SEQ ID NO:1. The transformation vector(KCTC 10474BP) using maize Ac/Ds transposable element for investigating a gene, a promoter or an enhancer is provided, wherein the maize Ac/Ds transposable element has the nucleotide sequence set forth in SEQ ID NO:2.
    • 目的:提供使用玉米Ac / Ds转座因子转化基因,启动子或增强子的转化载体,与以前的转座子标记载体相比,这些载体容易产生增强子系,并插入更多数量的单基因组 以获得许多无框架载体,使得它们可用于开发与提高生产力,抗病性或植物质量有关的基因或启动子。 构成:提供了用于研究基因,启动子或增强子的使用玉米Ac(活化剂(转座酶))/ Ds(解离)转座元件的转化载体(KCTC10475BP),其中玉米Ac / Ds转座元件具有核苷酸序列 如SEQ ID NO:1所示。 提供了使用玉米Ac / Ds转座元件研究基因,启动子或增强子的转化载体(KCTC10474BP),其中玉米Ac / Ds转座元件具有SEQ ID NO:2所示的核苷酸序列。