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2. 发明公开

KR1020070059373A ＮＤＲＧ2 단백질에 대한 단일클론항체 및 단백질 칩을이용한 정량 측정법 失效
标题翻译：单克隆抗体制备N-MYC下游调控基因2和使用蛋白质芯片确定NDRG2
公开(公告)号：KR1020070059373A
公开(公告)日：2007-06-12
申请号：KR1020050118140
申请日：2005-12-06
申请人： 한국생명공학연구원
发明人： 송은영 , 김재화 , 이희구 , 임종석 , 최승철 , 조미영 , 백경은 , 김진숙 , 김종태 , 윤도영 , 권두한 , 박미영 , 염영일 , 최용경 , 김영준
IPC分类号： C07K16/00 , C12N5/12 , C12Q1/00
CPC分类号： C07K16/18 , G01N33/574 , G01N33/6842 , G01N2500/00
摘要： A preparation method of a monoclonal antibody to an N-myc downstream regulated gene 2(NDRG2) protein and a quantitative determination of the NDRG2 protein by using a protein chip are provided to analyze characteristics of dendritic cells, measure presence of NDRG2 protein in cells or tissues, and study on the function of NDRG2 protein in cancer cells. The monoclonal antibody to N-myc downstream regulated gene 2(NDRG2) protein expressed from the dendritic cells which are differentiated from human peripheral monocytes or stem cells of umbilical cord blood is provided, wherein the subclass of the monoclonal antibody is IgG2a; and the monoclonal antibody is produced from a hybridoma cell line NDRG2-19C-4(KCTC 10854BP). The NDRG2 protein is determined quantitatively or qualitatively by treating a reverse-phase protein microarray having biological samples as a protein chip with the monoclonal antibody, wherein the biological sample is tissue, cell, whole blood, serum, plasma, saliva, cerebrospinal fluid or urine.
摘要翻译：提供了通过使用蛋白质芯片对N-myc下游调节基因2（NDRG2）蛋白的单克隆抗体的制备方法和NDRG2蛋白质的定量测定，以分析树突状细胞的特征，测定细胞中NDRG2蛋白的存在或组织，研究NDRG2蛋白在癌细胞中的功能。提供了从与人周边单核细胞或脐带血干细胞分化的树突状细胞表达的N-myc下游调节基因2（NDRG2）蛋白的单克隆抗体，其中单克隆抗体的亚类为IgG2a; 并且从杂交瘤细胞系NDRG2-19C-4（KCTC 10854BP）产生单克隆抗体。通过用单克隆抗体处理具有生物样品作为蛋白质芯片的反相蛋白质微阵列来定量或定性测定NDRG2蛋白质，其中生物样品是组织，细胞，全血，血清，血浆，唾液，脑脊髓液或尿液。

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