专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明公开

KR1020040038336A ＤＤＲ２ 티로신 키나아제 활성의 변형 방법 및 이와 같은방법으로 변형된 키나아제 활성을 갖는 ＤＤＲ２ 단백질 失效
标题翻译：用于修饰DDR2（DISCOIDIN DOMAIN RECEPTOR）酪氨酸激酶活性的方法和具有改良的激酶活性的DDR2蛋白
公开(公告)号：KR1020040038336A
公开(公告)日：2004-05-08
申请号：KR1020020067233
申请日：2002-10-31
申请人： 한국과학기술연구원 , 제일파마홀딩스 주식회사
发明人： 양범석 , 김정학 , 양경미
IPC分类号： C12N9/12
CPC分类号： C12N9/1205 , C12Y207/01112
摘要： PURPOSE: A method for modifying DDR2(discoidin domain receptor) tyrosine kinase activity and DDR2 protein having modified kinase activity thereby are provided, thereby treating diseases caused by oxidative stresses and over-activation of DDR2 tyrosine kinase. CONSTITUTION: A method for modifying DDR2(discoidin domain receptor) tyrosine kinase activity comprises the steps of: amplifying cDNA encoding the amino acid sequence containing the DDR2 kinase active site and connecting the amplified sequence to the C-terminal coding region of glutathione-S-transferase(GST) to produce DDR2 kinase-GST fusion gene; cloning the DDR2 kinase-GST fusion gene into an expression vector, expressing the tyrosine kinase active site of DDR2 protein-GST fusion protein in a host cell, and purifying the protein; and adding oxidative stresses to the fusion protein to phosphorylate tyrosine in the tyrosine kinase active site of DDR protein.
摘要翻译：目的：提供一种改变DDR2（盘状结构域受体）酪氨酸激酶活性的方法，从而提供具有修饰的激酶活性的DDR2蛋白，从而治疗由氧化应激引起的疾病和DDR2酪氨酸激酶的过度激活。构成：修饰DDR2（盘状结构域受体）酪氨酸激酶活性的方法包括以下步骤：扩增编码含有DDR2激酶活性位点的氨基酸序列的cDNA，并将扩增的序列连接到谷胱甘肽-S- 转移酶（GST）产生DDR2激酶-GST融合基因; 将DDR2激酶-GST融合基因克隆到表达载体中，在宿主细胞中表达DDR2蛋白-GST融合蛋白的酪氨酸激酶活性位点，并纯化该蛋白; 并向融合蛋白中加入氧化应激以磷酸化DDR蛋白酪氨酸激酶活性位点中的酪氨酸。

IPRDB

热门服务

关于我们

友情链接

联系方式