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    • 10. 发明公开
    • 나비목 해충에 대한 살충활성이 향상된 신규의 바실러스 투린지엔시스 내독소단백질을 코딩하는 유전자 및 그 제조방법
    • 新型巴豆酵母基因编码具有改善的左旋多巴素类药物的杀虫活性的晶体蛋白及其制备方法
    • KR1020140117257A
    • 2014-10-07
    • KR1020140004841
    • 2014-01-15
    • 서울대학교산학협력단
    • 제연호최재영김송은김재수
    • C12N15/32C12N15/63C12N15/70C12Q1/68
    • C07K14/325A01N63/02C12N15/8286Y02A40/162C12N15/11C12N15/63C12N15/70
    • The present invention relates to a novel bacillus thuringiensis endotoxic protein gene with improved insecticidal activities against lepidopteran insects and to a method for producing the same. More specifically, the present invention relates to a method for producing a bacillus thuringiensis endotoxic gene with strong insecticidal activities against lepidopteran insects such as Mamestra brassicae, Spodoptera litura, and Spodoptera exigua. The purpose of the present invention is achieved through the steps of: confirming a base sequence and a tertiary structure of a mod-cry1AC gene; producing a PIM-mod-cry1AC vector using the mod-cry1AC gene obtained from the previous step; producing a mutation-inducing primer using the mod-cry1AC as a mold; performing a polymerase chain reaction (PCR) using the mutation-inducing primer obtained from the previous step so as to produce novel BT endotoxic protein gene to be transformed in colon bacilli and confirming mutations; and confirming a base sequence and a tertiary structure of the novel BT endotoxic protein gene obtained from the previous step. Accordingly, in the present invention, agricultural products, which are not damaged by larvae of various lepidopteran insects, can be produced using a microorganism formulation including novel recombinant endotoxic protein gene Mut-N16.
    • 本发明涉及一种具有改善的对鳞翅目昆虫的杀虫活性的新型苏云金芽孢杆菌内毒素蛋白基因及其制备方法。 更具体地说,本发明涉及一种对鳞翅目昆虫(如Mamestra brassicae),斜纹夜蛾(Spodoptera litura)和斜纹夜蛾(Spodoptera exigua)具有强杀虫活性的苏云金芽孢杆菌内毒素基因的生产方法。 通过以下步骤实现本发明的目的:确认mod-cry1AC基因的碱基序列和三级结构; 使用从前一步获得的mod-cry1AC基因产生PIM-mod-cry1AC载体; 使用mod-cry1AC作为模具产生突变诱导引物; 使用从前一步骤获得的突变诱导引物进行聚合酶链反应(PCR),以产生要在大肠杆菌中转化的新的BT内毒素蛋白基因并确认突变; 并确认从前一步骤获得的新型BT内毒素蛋白质基因的碱基序列和三级结构。 因此,在本发明中,可以使用包含新型重组内毒素蛋白质基因Mut-N16的微生物制剂来制造未被各种鳞翅目昆虫的幼虫损伤的农产品。