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    • 1. 发明公开
    • 돼지 설사병 저항성 유전자 검정방법
    • 抗性基因检测方法与PORCINE EPIDEMIC DIARRHEA
    • KR1020020080504A
    • 2002-10-26
    • KR1020010019282
    • 2001-04-11
    • 최윤재이승호조광근
    • 최윤재조광근이승호김철욱
    • C12Q1/68
    • PURPOSE: Provided is a detection method of resistance gene against porcine epidemic diarrhea by examining resistance and susceptibility against Escherichia coli F18 receptor. CONSTITUTION: The detection method is characterized by the steps of: preparing primers for detecting mutation of FUT1 gene; and detecting a pig, which shows resistance against porcine epidemic diarrhea, by PCR using the prepared primers and a test sample collected from a pig. Wherein, the primers are selected from Int 1(SEQ ID NO:1), Int 2(SEQ ID NO:2), Ext 1(SEQ ID NO:3), Ext 2(SEQ ID NO:4) and Ext 2-2(SEQ ID NO:5); and PCR products are subjected to agarose gel electrophoresis.
    • 目的:通过检测对大肠杆菌F18受体的耐药性和易感性,提供了针对猪流行性腹泻的抗性基因的检测方法。 构成:检测方法的特点是:制备检测FUT1基因突变的引物; 通过使用制备的引物的PCR和从猪收集的试验样品检测显示出对猪流行性腹泻的抗性的猪。 其中引物选自Int 1(SEQ ID NO:1),Int 2(SEQ ID NO:2),Ext 1(SEQ ID NO:3),Ext 2(SEQ ID NO:4)和Ext 2- 2(SEQ ID NO:5); PCR产物进行琼脂糖凝胶电泳。
    • 2. 发明授权
    • 돼지 설사병 저항성 유전자 검정방법
    • 돼지설사병저항성유전자검정방법
    • KR100432588B1
    • 2004-05-24
    • KR1020010019282
    • 2001-04-11
    • 최윤재이승호조광근
    • 최윤재조광근이승호김철욱
    • C12Q1/68
    • PURPOSE: Provided is a detection method of resistance gene against porcine epidemic diarrhea by examining resistance and susceptibility against Escherichia coli F18 receptor. CONSTITUTION: The detection method is characterized by the steps of: preparing primers for detecting mutation of FUT1 gene; and detecting a pig, which shows resistance against porcine epidemic diarrhea, by PCR using the prepared primers and a test sample collected from a pig. Wherein, the primers are selected from Int 1(SEQ ID NO:1), Int 2(SEQ ID NO:2), Ext 1(SEQ ID NO:3), Ext 2(SEQ ID NO:4) and Ext 2-2(SEQ ID NO:5); and PCR products are subjected to agarose gel electrophoresis.
    • 目的:通过检测对大肠杆菌F18受体的抗性和易感性,提供一种抗猪流行性腹泻抗性基因的检测方法。 构成:该检测方法的特征在于以下步骤:制备检测FUT1基因突变的引物; 并使用所制备的引物和从猪收集的测试样品通过PCR检测对猪流行性腹泻具有抗性的猪。 其中,引物选自Int 1(SEQ ID NO:1),Int 2(SEQ ID NO:2),Ext 1(SEQ ID NO:3),Ext 2(SEQ ID NO:4)和Ext 2- 2(SEQ ID NO:5); 并对PCR产物进行琼脂糖凝胶电泳。
    • 4. 发明授权
    • 소의 조직특이유전자의 발현 측정용 DNA 칩 및 그 용도
    • 用于检测牛中组织特异性基因表达的DNA芯片及其用途
    • KR100673431B1
    • 2007-01-24
    • KR1020040103236
    • 2004-12-08
    • 조광근한경호최윤재
    • 조광근한경호최윤재김철욱백명기류언정
    • C12Q1/68
    • 본 발명은 소의 조직특이유전자의 발현 측정용 DNA 칩과 그 용도에 관한 것으로, 본 발명에서 제공하는 소의 조직특이유전자의 발현 측정용 cDNA 칩은 종래의 유전자 분석방법에 비하여 단축된 시간 내에 다량의 유전자 발현의 차이를 분석함으로써 유용한 유전자의 발굴의 도구로서 다양한 동물 유전자 실험에 효율적으로 이용될 수 있는 뛰어난 효과가 있다. 또한, 본 발명 소의 조직특이유전자의 발현 측정용 cDNA 칩을 이용하여 한우의 조직 특이적으로 발현되는 유전자의 발현 증가 또는 감소 여부를 동시에 탐색할 수 있는 방법을 제공하는 효과가 있다. 또한, 본 발명 소의 조직특이유전자의 발현 측정용 cDNA 칩은 등심조직 또는 등지방 특이적으로 발현되는 유전자를 탐색할 수 있으므로 이를 이용하여 한우의 육질을 평가할 수 있다. 따라서, 본 발명 소의 조직특이유전자의 발현 측정용 cDNA 칩은 국제적 경쟁력뿐만 아니라 상품화함으로서 오는 수입대체 및 수출증대 효과도 매우 클 것으로 예상된다.
      유전자 칩, DNA 칩, cDNA 칩, 소, 육질
    • 6. 发明授权
    • 스트레스감수성돼지의검정방법
    • 磷胁迫综合征(PSS)检测方法
    • KR100273656B1
    • 2001-01-15
    • KR1019980003517
    • 1998-02-06
    • 김철욱조광근최윤재
    • 김철욱최윤재조광근박외선권은정
    • C12Q1/68
    • PURPOSE: Provided is a method for accurately and rapidly detecting porcine stress syndrome by preparing a primer, amplifying DNA by PCR and confirming genotype by electrophoresis. CONSTITUTION: The detection method comprises: extracting DNA from the tissue and blood of a PSS pig; preparing 4 primers, Int 1(Internal 1), Int 2(Internal 2), Ext 1(External 1) and Ext 2(External 2), in 3 different ways from the specific part of nucleotide sequence in CRC 1gene of the calcium discharge channel ryanodine receptor 1; performing PCR by reacting 4 primers in one tube or reacting a pair of Ext 1 with Int 2 primer and a pair of Ext 2 in different tube respectively; and confirming by electrophoresis the band pattern of genotype according to the differences in size of amplified DNA fragments.
    • 目的:提供一种通过制备引物,通过PCR扩增DNA并通过电泳确认基因型来准确快速检测猪应激综合征的方法。 构成:检测方法包括:从PSS猪的组织和血液中提取DNA; 制备4种引物,Int 1(内部1),Int 2(内部2),Ext 1(外部1)和Ext 2(外部2),以不同的方式从CRC核苷酸序列的特定部分钙离子 通道兰诺定受体1; 通过在一个管中反应4个引物或分别使一对Ext1与Int2引物和一对Ext2反应来进行PCR; 并根据扩增的DNA片段的大小差异,通过电泳证实基因型的条带模式。
    • 9. 发明授权
    • 포유동물의 세포퇴화 관련 유전자의 염기서열
    • PBLUESCRIPT-MDF的基本序列
    • KR100261869B1
    • 2000-08-01
    • KR1019970052590
    • 1997-10-14
    • 최윤재
    • 김영민이주용고윤성하석호최윤재김형기이대연
    • C12N15/12
    • PURPOSE: The base sequence of a gene related to cell degeneration of mammals is provided, which is useful in figuring out the causes of breast cancer and diagnosing the disease more correctly. CONSTITUTION: The base sequence of a gene related to cell degeneration is obtained by the following steps of: i) preparing mammary gland cells; ii) obtaining total RNA from the degenerated cells; iii) making cDNA library; iv) performing mRNA differential display PCR to identify kinds of mRNAs which are differently expressed during the degeneration of mammary gland cells; v) performing Northern blotting with PCR products to identify the patterns of gene expression; vi) performing TA-cloning with PCR fragments using pGEM-T vector and sequencing the cloned cDNA fragments; and vii) screening cDNA and analyzing base sequences.
    • 目的:提供与哺乳动物细胞变性有关的基因的碱基序列,可用于确定乳腺癌的病因和诊断疾病。 构成:通过以下步骤获得与细胞变性相关的基因的碱基序列:i)制备乳腺细胞; ii)从退化细胞获得总RNA; iii)制作cDNA文库; iv)进行mRNA差异显示PCR以鉴定在乳腺细胞退化过程中不同表达的mRNA的种类; v)用PCR产物进行Northern印迹以鉴定基因表达的模式; vi)使用pGEM-T载体用PCR片段进行TA克隆,并测序克隆的cDNA片段; 和vii)筛选cDNA并分析碱基序列。
    • 10. 发明授权
    • 우유중 CLA 함량을 높이는 사료 및 그 제조방법
    • CLA CLA CLA CLA CLA CLA CLA CLA법법법법법법법법법법법법법
    • KR100431906B1
    • 2004-05-17
    • KR1020010063661
    • 2001-10-16
    • 주식회사 카길애그리퓨리나최윤재
    • 최윤재이보균이홍구
    • A23K1/00
    • PURPOSE: A method of preparing the titled feed by adding heat treated full fat soybean and linseed oil to feed and then heat treating the obtained feed for dairy cow within a short time is provided. Thus obtained feed is suitable for normal digestive physiology of diary cows which increase the CLA content of cow's milk without reduction of milk fat and the quantity of milk. CONSTITUTION: The titled feed is prepared by mixing 25% by weight of heat treated full fat soybean with a mixture and then heat treating at 120 to 150deg.C for 0.2 to 9.4sec to produce feed. The mixture contains 7 to 16% by weight of processed corn, 6 to 12% by weight of wheat, 7 to 18% by weight of a corn by-product, 0.01 to 3% by weight of defatted bran, 6 to 9% by weight of soybean pod, 3 to 7% by weight of lupin skin, 1 to 5% by weight of wheat flour, 1 to 7% by weight of wheat bran, 17 to 23% by weight of vegetable meals, 1 to 2% by weight of limestone, 0.1 to 1.0% by weight of defluorinated phosphoric acid, 0.5 to 1.5% by weight of salt, 5 to 7% by weight of molasses, 0.1 to 0.2% by weight of mineral additives and 0.01 to 0.1% by weight of vitamin additives.
    • 目的:提供一种通过加入热处理后的全脂大豆和亚麻子油来饲喂并且然后在短时间内对获得的用于奶牛的饲料进行热处理来制备标题饲料的方法。 这样获得的饲料适合日常奶牛的正常消化生理学,其增加牛奶中的CLA含量而不减少乳脂肪和牛奶的量。 组成:标题饲料是通过将25重量%热处理过的全脂大豆与一种混合物混合,然后在120至150℃下热处理0.2至9.4秒以制备饲料来制备的。 该混合物含有7-16重量%的加工玉米,6-12重量%小麦,7-18重量%玉米副产物,0.01-3重量%脱脂麸皮,6-9重量% 大豆荚重量,3至7重量%羽扇豆皮,1至5重量%小麦粉,1至7重量%小麦麸皮,17至23重量%蔬菜粉,1至2重量% 石灰石的重量,0.1至1.0重量%的脱氟磷酸,0.5至1.5重量%的盐,5至7重量%的糖蜜,0.1至0.2重量%的矿物添加剂和0.01至0.1重量%的 维生素添加剂