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    • 3. 发明公开
    • 감자난초와 두잎감자난초의 감별 방법 및 키트
    • 用于区分OREORCHIS PATENS和OREORCHIS COREANA之间的方法和工具包
    • KR1020130022313A
    • 2013-03-06
    • KR1020110085659
    • 2011-08-26
    • 재단법인 제주테크노파크
    • 오대주송관필정용환박소현최선아고명석
    • C12Q1/68C12N15/11
    • C12Q1/683C12Q1/6858C12Q2600/13C12Q2600/156
    • PURPOSE: A method and a kit for discriminating Oreorchis patens and Oreorchis coreana Finet are provided to easily discriminate the Oreorchis patens and the Oreorchis coreana Finet by using a direct sequencing method, an SNP-genotyping method, and an RFLP(Restriction fragment length polymorphism) method. CONSTITUTION: A method for discriminating Oreorchis patens and Oreorchis coreana Finet comprises: a step of preparing biological samples from the Oreorchis patens and the Oreorchis coreana Finet; a step of isolating genome DNA from the biological samples; a step of amplifying a DNA sequence containing a GCCGGC sequence, which is a recognition site of a Nael restriction enzyme in an ITS sequence of the Oreorchis coreana Finet; a step of amplifying a DNA sequence containing a GCNGGC sequence, which is an alternative sequence of the Oreorchis patens; and a step of treating the amplified product with NaeI.
    • 目的:提供一种用于区分Oreorchis patens和Oreorchis coreana Finet的方法和试剂盒,以便通过使用直接测序方法,SNP基因分型法和RFLP(限制性片段长度多态性)轻松区分Oreorchis patens和Oreorchis coreana Finet, 方法。 构成:一种鉴别Oreorchis patens和Oreorchis coreana Finet的方法包括:从Oreorchis patens和Oreorchis coreana Finet制备生物样品的步骤; 从生物样品中分离基因组DNA的步骤; 在Oreorchis coreana Finet的ITS序列中扩增含有作为Nael限制酶的识别位点的GCCGGC序列的DNA序列的步骤; 扩增含有作为Oreorchis patens的替代顺序的GCNGGC序列的DNA序列的步骤; 以及用NaeI处理扩增产物的步骤。