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2. 发明公开

KR1020040077002A 개선된 암세포 특이성과 활성을 가지는 사람 텔로미어역전사효소 프로모터 및 이를 포함하는 재조합 벡터 有权
标题翻译：具有增强的肿瘤特异性和强度的重组载体和包含其的重组载体选择性地杀死癌细胞
公开(公告)号：KR1020040077002A
公开(公告)日：2004-09-04
申请号：KR1020030012364
申请日：2003-02-27
申请人： 윤채옥 , 김주항
发明人： 윤채옥 , 김주항 , 양재명
IPC分类号： C12N15/52
CPC分类号： C12N15/86 , A61K38/45 , A61K48/00 , C12N9/1276 , C12N2710/10343 , C12N2830/008 , C12N2830/85
摘要： PURPOSE: An hTERT(human telomere reverse transcriptase) promoter with enhanced tumor-specificity and strength and a recombinant vector comprising the same are provided, thereby specifically and highly expressing the hTERT gene in only cancer cells. Therefore, cancer cells can be selectively killed. CONSTITUTION: A transcription regulating sequence comprises the hTERT promoter and one or more of c-Myc binding sites and/or one or more of Sp1 binding sites which are linked to the hTERT promoter, wherein the c-Myc binding site has the nucleotide sequence selected from SEQ ID NO: 2, SEQ ID NO:3 and SEQ ID NO:4; the Sp1 binding site has the nucleotide sequence selected from SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10 and SEQ ID NO:11; the regulating sequence has the nucleotide sequence set forth in SEQ ID NO:14. The recombinant vector comprises the transcription regulating sequence which is operably linked to the gene required for virus replication, wherein the virus vector is derived from Adenoviridiae; the gene required for virus replication is an initial gene E1A.
摘要翻译：目的：提供具有增强的肿瘤特异性和强度的hTERT（人端粒逆转录酶）启动子和包含其的重组载体，从而特异性且高度表达仅在癌细胞中的hTERT基因。因此，可以选择性地杀死癌细胞。构成：转录调节序列包含hTERT启动子和c-Myc结合位点和/或与hTERT启动子连接的Sp1结合位点中的一个或多个的一个或多个，其中c-Myc结合位点具有选择的核苷酸序列从SEQ ID NO：2，SEQ ID NO：3和SEQ ID NO：4; Sp1结合位点具有选自SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10和SEQ ID NO： 11; 调节序列具有SEQ ID NO：14所示的核苷酸序列。重组载体包含可操作地连接到病毒复制所需的基因的转录调控序列，其中病毒载体衍生自腺病毒; 病毒复制所需的基因是初始基因E1A。

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