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1. 发明公开

KR1020110042517A 하이드로젤을 이용한 나노다공성 박막에의 세포 마이크로패터닝 失效
标题翻译：使用HYDROGEL MICROWELL在纳米膜上进行细胞培养
公开(公告)号：KR1020110042517A
公开(公告)日：2011-04-27
申请号：KR1020090099215
申请日：2009-10-19
申请人： 연세대학교 산학협력단
发明人： 고원건 , 김대년
IPC分类号： B82B1/00 , B82B3/00
CPC分类号： G01N33/68 , C12Q1/6837 , G01N33/528
摘要： PURPOSE: A cell micro-patterning with respect to a nano-porous thin film using hydrogel is provided to improve the adhesion of cells on a substrate by micro patterning the hydrogel on the surface of the thin film with a nanotopology. CONSTITUTION: A nano-structure includes a porous substrate and hydrogel. The hydrogel in a well shape is patterned on the substrate. The substrate is aluminum oxide porous thin film. The hydrogen is obtained based on one or more polymers selected from a group including polyacrylic acid, polyacrylamide, polyhydroxylethyl methacrylate, polyethylene glycol, the poly(N,N-methylaminoethylmethacrylate), hyaluronic acid, and chitosan.
摘要翻译：目的：提供相对于使用水凝胶的纳米多孔薄膜的微图案化，以通过用纳米拓扑显微图案化薄膜表面上的水凝胶来改善细胞在基底上的粘附。构成：纳米结构包括多孔底物和水凝胶。在衬底上形成井形状的水凝胶。基板是氧化铝多孔薄膜。基于一种或多种选自聚丙烯酸，聚丙烯酰胺，聚羟基乙基甲基丙烯酸酯，聚乙二醇，聚（N，N-甲基氨基乙基甲基丙烯酸酯），透明质酸和壳聚糖的聚合物获得氢。

2. 发明公开

KR1020100021929A 폴리에틸렌 글리콜 하이드로젤 미세구조에의 3차원적 단백질 패터닝 방법 无效
标题翻译：三维聚乙二醇（乙二醇）水凝胶微结构表面蛋白质的微观分析
公开(公告)号：KR1020100021929A
公开(公告)日：2010-02-26
申请号：KR1020080080602
申请日：2008-08-18
申请人： 연세대학교 산학협력단
发明人： 고원건 , 김대년 , 박진원
IPC分类号： C08J3/075 , C08J7/04 , C08G65/26 , G01N33/44
摘要： PURPOSE: A surface-modified poly(ethylene glycol) hydrogel microstructure is provided to chemically bond protein on the surface of a hydrogel and to reduce the amount of necessary proteins by bonding proteins on the only surface of the hydrogel without infiltration topenetrating the inside. CONSTITUTION: A method for manufacturing a surface-modified hydrogel comprises the following steps of: preparing a poly(ethylene glycol) hydrogel microstructure; and modifying the surface of a hydrogel by reacting the poly(ethylene glycol) hydrogel microstructure with a compound of chemical formula 1: L1-L2-L3. In chemical formula 1, L1 is a functional group capable of creating a covalent bond with poly(ethylene glycol); L2 is an organic group; and L3 is a functional group capable of creating a covalent bond with a protein moiety.
摘要翻译：目的：提供表面改性的聚（乙二醇）水凝胶微结构，以在水凝胶的表面上化学键合蛋白质，并通过在水凝胶的唯一表面上键合蛋白质来减少所需蛋白质的量，而不会渗透到内部。构成：制备表面改性水凝胶的方法包括以下步骤：制备聚（乙二醇）水凝胶微观结构; 并通过使聚（乙二醇）水凝胶微结构与化学式1：L1-L2-L3的化合物反应来改性水凝胶的表面。在化学式1中，L1是能够与聚（乙二醇）形成共价键的官能团; L2是有机基团; 并且L3是能够与蛋白质部分形成共价键的官能团。

3. 发明公开

KR1020110024012A 효소 및 양자점을 함유하는 하이드로겔을 포함하는 페놀 또는 그 유도체 센서 및 이를 이용한 페놀 또는 그 유도체의 검출방법 无效
标题翻译：用于检测包含含氢量和酶和酚类或酚醛衍生物的氢化物的酚或衍生物的传感器 - 使用其的检测方法
公开(公告)号：KR1020110024012A
公开(公告)日：2011-03-09
申请号：KR1020090081851
申请日：2009-09-01
申请人： 연세대학교 산학협력단 , 홍익대학교 산학협력단
发明人： 고원건 , 장은지 , 김대년 , 김범상
IPC分类号： C12Q1/26 , C12Q1/42
CPC分类号： C12Q1/26 , C07C39/04 , C09K11/08 , C12N11/02 , C12Q1/42 , C12Y101/03004 , C12Y111/01007 , C12Y114/18001 , C12Y301/03001
摘要： PURPOSE: A sensor for detecting phenol or phenol derivative and a detection method using the same are provided to enable chemical process and environmental monitoring. CONSTITUTION: A sensor for detecting phenol or phenol derivative of chemical formula I contains a hydrogel in which enzyme and quantum dot are fixed. The enzyme is tyrosinase, glucose oxidase, phosphatase, or hydrogen peroxidase. The quantum dot(QD) is CdSe-ZnS QD, CdS QD, CdTe QD, CdSe QD, ZnS QD, ZnSe QD, or PbSe QD. The hydrogel is obtained by UV polymerization of photo crosslinking agent under the presence of photopolymerization initiator. The photo crosslinking agent is biodegradable polymer having photopolymerization functional group. A method for detecting phenol or phenol derivative of chemical formula I comprises: a step of mixing enzyme, quantum dot, photo crosslinking agent, and photo polymerization initiator; and a step of curing the mixture through UV irradiation.
摘要翻译：目的：提供用于检测苯酚或苯酚衍生物的传感器和使用该传感器的检测方法，以实现化学过程和环境监测。构成：用于检测化学式I的苯酚或苯酚衍生物的传感器含有固定有酶和量子点的水凝胶。该酶是酪氨酸酶，葡萄糖氧化酶，磷酸酶或过氧化氢酶。量子点（QD）是CdSe-ZnS QD，CdS QD，CdTe QD，CdSe QD，ZnS QD，ZnSe QD或PbSe QD。水凝胶通过光聚合引发剂存在下光交联剂的UV聚合获得。光交联剂是具有光聚合官能团的生物降解聚合物。检测化学式I的苯酚或苯酚衍生物的方法包括：将酶，量子点，光交联剂和光聚合引发剂混合的步骤; 以及通过UV照射固化该混合物的步骤。

4. 发明授权

KR100836206B1 패턴형성용 폴리에틸렌글리콜 하이드로젤과 이의 제조방법 失效
标题翻译：用于制造微波炉的PEG氢化物及其工艺
公开(公告)号：KR100836206B1
公开(公告)日：2008-06-09
申请号：KR1020070027370
申请日：2007-03-20
申请人： 연세대학교 산학협력단
发明人： 고원건 , 류두열 , 이우진 , 최동길 , 이열 , 김대년
IPC分类号： C08G65/32 , C08G65/325 , C08G65/00 , G02F1/13
摘要： A polyethylene glycol hydrogel for forming a micropattern is provided to allow chemical bonding with proteins, and to fix the proteins firmly according to a pattern when a micropattern is formed by using photolithography. A method for preparing polyethylene glycol hydrogel for a micropattern includes the steps of: (a) introducing benzophenone to polyethylene glycol hydrogen, and grafting an acrylic compound having a carboxyl group or amide group onto the benzophenone layer to introduce the functional groups to the surface of the polyethylene glycol hydrogel; (b) activating the functional groups introduced to the surface of the polyethylene glycol hydrogel; and (c) forming an amino bond between the activated functional groups and a protein to fix the protein to the surface of the polyethylene glycol hydrogel.
摘要翻译：提供用于形成微图案的聚乙二醇水凝胶，以通过使用光刻法形成微图案，从而与蛋白质进行化学键合，并且可以根据图案将蛋白质牢固地固定。制备用于微图案的聚乙二醇水凝胶的方法包括以下步骤：（a）将二苯甲酮引入聚乙二醇氢中，并将具有羧基或酰胺基的丙烯酸化合物接枝到二苯甲酮层上以将官能团引入到聚乙二醇水凝胶; （b）活化引入聚乙二醇水凝胶表面的官能团; 和（c）在活化的官能团和蛋白质之间形成氨基键以将蛋白质固定在聚乙二醇水凝胶的表面。

5. 发明授权

KR100934646B1 비구형 하이드로 젤 미세입자를 기반으로 한 바이오 분석용미세유체시스템 失效
标题翻译： 비구형하이드로젤미세입자를기반으로한바이오분석용미세체체시스템
公开(公告)号：KR100934646B1
公开(公告)日：2009-12-31
申请号：KR1020080009781
申请日：2008-01-30
申请人： 연세대학교 산학협력단
发明人： 고원건 , 박진원 , 최동길 , 이우진 , 김대년 , 이열
IPC分类号： G01N35/00
摘要： 본 발명은 비구형 하이드로 젤 미세입자를 기반으로 한 바이오 분석용 미세유체시스템에 관한 것으로, 포토리소그래피를 이용하여 비구형 하이드로 젤 미세입자를 제조하는 하이드로 젤 합성 부분과 소수성의 표면처리로 인해 합성된 하이드로 젤을 칩의 표면으로부터 쉽게 분리하여 원하는 위치에 고정할 수 있는 신호 분석 부분을 포함하도록 제조된 바이오 분석용 미세유체시스템에 관한 것이다.
미세유체시스템, 하이드로 젤, 포토리소그래피, 생체물질
摘要翻译：提供微流体系统以合成含各种形状的各种生物材料的水凝胶微粒，并将水凝胶微粒固定到信号分析室。微流体系统包括一个或多个微流体通道。微流体通道包括通过使用从外部流动的流体合成水凝胶颗粒的聚合室; 通过使用水凝胶颗粒分析信号并连接到水凝胶合成室的检测室; 用于将流体供应到合成室的流体入口; 以及用于将从分析室流出的流体排出到外部的流体出口。

6. 发明公开

KR1020090083784A 비구형 하이드로 젤 미세입자를 기반으로 한 바이오 분석용미세유체시스템 失效
标题翻译：合并非生物水文微生物微生物装置
公开(公告)号：KR1020090083784A
公开(公告)日：2009-08-04
申请号：KR1020080009781
申请日：2008-01-30
申请人： 연세대학교 산학협력단
发明人： 고원건 , 박진원 , 최동길 , 이우진 , 김대년 , 이열
IPC分类号： G01N35/00
摘要： A microfluidic system is provided to synthesize hydrogel microparticles containing various biomaterials to various shapes, and to fix the hydrogel microparticles to a signal analysis chamber. A microfluidic system comprises one or more microfluidic channels. The microfluidic channel includes a polymerization chamber which synthesizes hydrogel particle by using fluid flowed from the outside; a detection chamber which analyzes signal by using the hydrogel particles and is connected to the hydrogel synthesis chamber; a fluid inlet for supplying the fluid to synthetic chamber; and a fluid outlet for discharging the fluid flown from the analysis chamber to the outside.
摘要翻译：提供微流体系统以合成含有各种生物材料的各种形状的水凝胶微粒，并将水凝胶微粒固定在信号分析室上。微流体系统包括一个或多个微流体通道。微流体通道包括通过使用从外部流出的流体合成水凝胶颗粒的聚合室; 检测室，其通过使用水凝胶颗粒分析信号并连接到水凝胶合成室; 用于将流体供应到合成室的流体入口; 以及用于将从分析室流出的流体排出到外部的流体出口。

7. 发明公开

KR1020080084432A 포토리소그래피를 이용한 다공성 생체적합 고분자 하이드로겔과 이를 이용한 효소반응기 无效
标题翻译：微生物水凝胶膜的制备用于酶固定的应用
公开(公告)号：KR1020080084432A
公开(公告)日：2008-09-19
申请号：KR1020070026148
申请日：2007-03-16
申请人： 연세대학교 산학협력단
发明人： 박진원 , 고원건 , 최동길 , 이우진 , 이열 , 김대년
IPC分类号： C12N11/08 , C12N11/00
摘要： A fabrication method of micropatterned hydrogel membrane is provided to improve uniformity and penetration of pores in the hydrogel membrane by using photomask, so that the enzyme is physicochemically immobilized to the micropatterned hydrogel membrane strongly. A fabrication method of micropatterned hydrogel membrane having tunnel-like pores at regular distances comprises the steps of: mixing 10-90 wt.% of biocompatible polymer with vinyl group in both ends, 10-90 wt.% of vinyl group derivative, 0.1-2 wt.% of photoinitiator and 0.1-2 wt.% of linker, and introducing the mixture into a mold; and exposing the molded membrane to ultraviolet rays in a range of 0.01-10 WATT for 1-60 seconds through photomask, wherein the hydrogel has specific surface area of 100-200 mm^2 and thickness of 1-2000 mum; and the vinyl group is acryl group, styrene group or epoxy group. An enzyme reactor contains the hydrogel membrane as support.
摘要翻译：提供微图案水凝胶膜的制造方法，以通过使用光掩模来改善水凝胶膜中的孔的均匀性和渗透性，使得该酶强烈地物理化学固定在微图案化的水凝胶膜上。具有规则距离的具有隧道状孔的微图案化水凝胶膜的制造方法包括以下步骤：将10-90重量％的生物相容性聚合物与乙烯基两端混合，10-90重量％的乙烯基衍生物，0.1- 2重量％的光引发剂和0.1-2重量％的接头，并将混合物引入模具中; 并通过光掩模将成型的膜暴露于0.01-10WATT范围内的紫外线，持续1-60秒，其中水凝胶的比表面积为100-200mm ^ 2，厚度为1-2000μm; 乙烯基为丙烯酰基，苯乙烯基或环氧基。酶反应器含有作为载体的水凝胶膜。

8. 发明授权

KR101116803B1 하이드로젤을 이용한 나노다공성 박막에의 세포 마이크로패터닝 失效
标题翻译：使用水凝胶微孔的纳米多孔膜上的细胞微图案
公开(公告)号：KR101116803B1
公开(公告)日：2012-02-29
申请号：KR1020090099215
申请日：2009-10-19
申请人： 연세대학교 산학협력단
发明人： 고원건 , 김대년
IPC分类号： B82B1/00 , B82B3/00
摘要： 본 발명은 하이드로젤을 이용한 나노다공성 박막에의 세포 마이크로패터닝에 관한 것으로, 보다 상세하게는, 다공성 박막 상에 웰 형태의 하이드로젤이 패터닝되어 있는 나노구조체로서, 상기 하이드로젤은 기질의 표면 개질없이 패터닝될 수 있고, 상기 구조체에는 세포가 효과적으로 부착할 수 있으며, 세포 분비물 등을 측정할 수 있는 바이오마커가 하이드로젤에 3차원 고정이 가능하여 검출신호의 강도를 높일 수 있어 바이오센서 등으로 사용할 수 있다.
하이드로젤, 다공성 박막, 바이오마커, 세포 패터닝

9. 发明公开

KR1020100101360A 반응 챔버와 분석 챔버를 포함하는 미세유체 장치 无效
标题翻译：包含反应室和分析室的微流体装置
公开(公告)号：KR1020100101360A
公开(公告)日：2010-09-17
申请号：KR1020090019814
申请日：2009-03-09
申请人： 연세대학교 산학협력단
发明人： 고원건 , 김대년 , 이열
IPC分类号： G01N33/50 , C12Q1/68
CPC分类号： G01N33/50 , C12Q1/6834 , C12Q1/6837
摘要： PURPOSE: A microfluid apparatus having microfluide channel unit with a reaction chamber and analysis chamber is provided to enhance reactivity with a material. CONSTITUTION: A microfluid apparatus has a microfluid channel unit comprising: a reaction chamber with microbead in which biomolecule which reacts with analysis subject; and an analysis chamber with a hydrogel microarray. The reaction chamber further comprises a filter which is able to block loss of microbead. The microfluid apparatus further comprises: a fluid inlet port for supplying fluid to the reaction chamber; and a fluid outlet port for discharging fluid to the outside. The hydrogel contains aqueous polymer and material which induces fluorescence emission or coloring by reacting with reaction product between the analysis subject and biomolecule.
摘要翻译：目的：提供具有反应室和分析室的具有微流体通道单元的微流体装置，以增强与材料的反应性。构成：微流体装置具有微流体通道单元，其包括：具有微珠的反应室，其中与分析对象反应的生物分子; 和具有水凝胶微阵列的分析室。反应室还包括能够阻止微珠损失的过滤器。微流体装置还包括：用于向反应室供应流体的流体入口; 以及用于将流体排放到外部的流体出口。水凝胶含有水性聚合物和材料，其通过与分析对象和生物分子之间的反应产物反应而诱导荧光发射或着色。

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