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    • 5. 发明公开
    • CYP4F16 유전자가 넉다운된 형질전환마우스 및 그 제조방법
    • CYP4F16 KNOCK-DOWN鼠标及其制造方法
    • KR1020130080572A
    • 2013-07-15
    • KR1020120001427
    • 2012-01-05
    • 대한민국(농촌진흥청장)
    • 양병철김경운정학재황성수오건봉이휘철박수봉정은주박진기황규찬김남형
    • C12N15/85C12N15/113A01K67/027
    • PURPOSE: A transgenic mouse in which CYP4F16 gene is knocked-down and a manufacturing method thereof are provided to inquire a cause of occurrence of a sleeping disease among immune-related genes generated after infestation of trypanosome and to develop new medicine for curing the diseases. CONSTITUTION: A siRNA expression recombinant vector for mouse CYP4F16 knocked-down is identified by a promoter and SEQ ID No. 1 that is operably connected to the promoter. The promoter is a U6 promoter. The recombinant vector is introduced to a transgenic mouse. A manufacturing method of a transgenic mouse in which CYP4F16 gene expression is knocked-down comprises the following steps of: (a) manufacturing a siRNA expression cassette for expressing CYP4F16 that is represented by a promoter and SEQ ID No. 1 that is operably connected to the promoter; (b) introducing the recombinant fused gene to a fertilized egg of a mouse; and (c) producing breeders by implanting the fertilized egg into a surrogate mother.
    • 目的:提供CYP4F16基因被敲除的转基因小鼠及其制造方法,用于询问在锥虫感染后产生的免疫相关基因中发生睡眠疾病的原因,并开发用于治疗疾病的新药。 构成:用于小鼠CYP4F16敲除的siRNA表达重组载体通过与启动子可操作连接的启动子和SEQ ID No.1来鉴定。 启动子是U6启动子。 将重组载体导入转基因小鼠。 其中CYP4F16基因表达被敲除的转基因小鼠的制造方法包括以下步骤:(a)制备用于表达CYP4F16的siRNA表达盒,所述siRNA表达盒由启动子和SEQ ID No.1表示,所述启动子和SEQ ID No.1可操作地连接到 启动子; (b)将重组融合基因导入小鼠受精卵; 和(c)通过将受精卵植入替代母亲来培育育种者。