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    • 2. 发明公开
    • 배추 게놈의 염색체별로 유일발현하는 단일발현 유전자마커
    • 根据BRASSICA RAPA的染色体显示的单一表达的基因标记
    • KR1020060110880A
    • 2006-10-26
    • KR1020050032626
    • 2005-04-20
    • 대한민국(농촌진흥청장)
    • 김정선박범석진용문김호일
    • C12N15/29
    • A single expression genetic marker that is expressed according to the chromosomes of Brassica rapa genome is provided to discriminate chromosomes of Brassica rapa and quantitatively analyze the genetically modified crops of Brassica rapa. The single expression genetic marker that is expressed according to the chromosomes of Brassica rapa genome comprises 64 kinds of single expression genes located according to the related groups of Brassica rapa, having the nucleotide sequences of SEQ ID NO:1 to SEQ ID NO:64, wherein the single expression genetic marker comprises combinations of at least two genes selected from the 64 kinds of single expression genes of SEQ ID NO:1 to SEQ ID NO:64; and the single expression genes are identified by analyzing the distribution positions of single expression genes in the chromosomes of Brassica rapa.
    • 提供根据芸苔属芸苔基因组染色体表达的单一表达遗传标记,以区分芸苔属的染色体,并定量分析芸苔属的转基因作物。 根据芸苔属芸苔基因组染色体表达的单表达遗传标记包含根据芸苔属相关组的64种单一表达基因,其具有SEQ ID NO:1至SEQ ID NO:64的核苷酸序列, 其中所述单一表达遗传标记包含选自SEQ ID NO:1至SEQ ID NO:64的64种单一表达基因的至少两种基因的组合; 并通过分析芸苔属染色体中单个表达基因的分布位置来鉴定单一表达基因。
    • 5. 发明授权
    • 배추의 배축을 이용한 재생방법 및 유용한 외래유전자로형질전환된 배추의 생산방법
    • 배추의배축을이용한법방법및유용한외래유전자로형질전환된배추의생산방배추
    • KR100375674B1
    • 2003-03-15
    • KR1020000039834
    • 2000-07-12
    • 대한민국(농촌진흥청장)
    • 조현석박범석진용문김호일
    • A01H4/00
    • PURPOSE: A method for regenerating cabbage using hypocotyl and a method for producing cabbage transformed with useful foreign gene are provided to establish a system which is allowed to introduce a foreign gene useful to cabbage. CONSTITUTION: The method for regenerating a cabbage comprises steps of germinating cabbage seed to prepare hypocotyl, isolating the hypocotyl, and regenerating a cabbage using the hypocotyl in the culture media containing silver nitrate(AgNO3). In the method for producing the transformed cabbage, the cabbage seed is firstly disinfected and germinated to prepare hypocotyl. And then, hypocotyl is isolated and pre-cultured. Expression vector comprising a foreign gene(e.g., Agrobacterium) is introduced into the hypocotyl, to allow hypocotyl to transform with the foreign gene. The hypocotyl is used to regenerate a transformed cabbage in the culture media containing silver nitrate, hereby transformed cabbage being produced.
    • 目的:提供一种利用下胚轴再生甘蓝的方法和转化有用外源基因的甘蓝生产方法,以建立允许引入用于甘蓝的外源基因的系统。 构成:使卷心菜再生的方法包括使卷心菜种子发芽以制备下胚轴,分离下胚轴和在含有硝酸银(AgNO3)的培养基中使用下胚轴再生卷心菜的步骤。 在转化甘蓝的生产方法中,先将甘蓝种子消毒并发芽以制备下胚轴。 然后分离下胚轴进行预培养。 将包含外源基因(例如农杆菌)的表达载体引入下胚轴中,以允许下胚轴与外源基因转化。 下胚轴用于在含有硝酸银的培养基中再生转化的卷心菜,由此转化生产的卷心菜。
    • 6. 发明公开
    • 배추과 작물의 품종 구분 및 유용표지인자 개발수단으로서의 왜성전이인자, 이를 이용한 DNA 표지인자제작용 프라이머, 및 이들을 이용한 배추과 작물의 품종구분방법
    • 大白菜和作物品种的分类,以及大白菜作物品种的分类作为开发有用标记的手段
    • KR1020070033780A
    • 2007-03-27
    • KR1020050088305
    • 2005-09-22
    • 대한민국(농촌진흥청장)
    • 양태진권수진박범석김정선임기병진민아김진아임명호박지영김호일진용문
    • C12Q1/68
    • C12N15/11C12Q1/6855C12Q1/686
    • 본 발명은 배추과 작물의 품종 구분 및 유용표지인자 개발 수단으로서의 왜성전이인자인 서열번호1 기재의 TRIM-Br1 또는 서열번호2 기재의 TRIM-Br2, 이를 이용한 배추과 작물의 품종 구분에 사용되는 DNA 표지인자 제작용 프라이머인 서열번호3~10 중 어느 하나의 서열을 갖는 Br1n2DP-R 또는 서열번호11~14 중 어느 하나의 서열을 갖는 Br1n2DP-L, 및 이를 이용한 배추과 작물의 품종 구분 방법에 관한 것으로서, 보다 상세하게는 배추과 작물의 유전육종 과정에서 유용한 표지인자를 탐색하거나 유전자 지도 작성 및 품종구분에 효율적으로 활용할 수 있는 것으로서 배추과 작물의 유전자 밀집지역 전반에 골고루 삽입되어 있는 TRIM-Br1 및 TRIM-Br2 전이인자, 상기 전이인자 TRIM-Br1 및 TRIM-Br2를 이용한 DNA 표지인자를 제작하는데 사용되는 프라이머, 및 TRIM-Br1 및 TRIM-Br2의 특이 염기서열을 이용한 배추과 작물의 품종 구분방법에 관한 것이다.
      배추, 왜성 전이인자, TRIM-Br1, TRIM-Br2, TRIM 디스플레이
    • 另外,本发明中的品种使用的DNA标记敏感的和有用的标记开发为什么作为一种手段TRIM-的Br 2 SEQ ID NO:2的寺院yiinja:在TRIM-BR1或SEQ ID NO描述1:2基板,品种区分使用相同baechugwa作物baechugwa作物 Br1n2DP-R或SEQ ID NO:具有任何SEQ ID NO:1的引物的序列:3-10使用相同制造具有任何序列的11〜14 Br1n2DP-L,并且涉及baechugwa作物的各种9分钟方式,并且更 特别是在农作物的或遗传育种过程baechugwa TRIM-BR1和TRIM-BR2的过渡因子导航有用的标记,以有效地利用遗传作图和品种9分钟,其被均匀地插入到baechugwa作物的整体遗传区域 ,使用转录因子TRIM-Br1和TRIM-Br2构建DNA标记的引物,以及用于构建TRIM-Br1和TRIM-Br2的引物 它涉及baechugwa的方法作物品种使用的碱基序列9分。
    • 7. 发明公开
    • 식물조직의 특이 프로모터 영역
    • 植物组织特殊促进剂
    • KR1020020072068A
    • 2002-09-14
    • KR1020010012040
    • 2001-03-08
    • 대한민국(농촌진흥청장)
    • 박범석진용문김호일이연희김현욱
    • C12N15/11
    • C07K14/415C12N15/8231
    • PURPOSE: Provided are plant tissue specific promoters, thereby controlling when, where and how much to express a foreign gene. CONSTITUTION: A pollen specific promoter region Pban102 contains 1,177 bp upstream of an initiation codon ATG in a genomic clone of BAN102 gene having the nucleotide sequence of SEQ ID NO: 1. A pollen superior promoter region Pban103 contains 1 to 1831 bp in a genomic clone of BAN103 gene having the nucleotide sequence of SEQ ID NO: 2. A pollen specific promoter region P84 contains 1 to 565 bp in a genomic clone of BAN84 gene having SEQ ID NO: 3. Plasmids, pGBAN84, p84Xb, p84Bm and p84SM, contain the genomic clone of Chinese cabbage containing the BAN84 gene. A carpet tissue specific promoter region PBcA9 contains 616 to 1,436 bp in a genomic clone of BcA9 gene having the nucleotide sequence of SEQ ID NO: 4. A vascular bundle specific promoter region Pbif38 contains 1 to 966 bp in a gemonic clone of BIF38 gene having the nucleotide sequence of SEQ ID NO: 5.
    • 目的:提供植物组织特异性启动子,从而控制表达外源基因的时间,位置和数量。 构成:花粉特异性启动子区域Pban102在具有SEQ ID NO:1的核苷酸序列的BAN102基因的基因组克隆中含有起始密码子ATG上游1177bp。花粉优势启动子区域Pban103在基因组克隆中含有1至1831bp 具有SEQ ID NO:2的核苷酸序列的BAN103基因。花粉特异性启动子区P84在具有SEQ ID NO:3的BAN84基因的基因组克隆中含有1至565bp。质粒pGBAN84,p84Xb,p84Bm和p84SM含有 含有BAN84基因的大白菜的基因组克隆。 地毯组织特异性启动子区域PBcA9在具有SEQ ID NO:4的核苷酸序列的BcA9基因的基因组克隆中含有616至1,436bp。血管束特异性启动子区域Pbif38在BIF38基因的宝石克隆中含有1至966bp, SEQ ID NO:5的核苷酸序列。
    • 8. 发明公开
    • 배추의 배축을 이용한 재생방법 및 유용한 외래유전자로형질전환된 배추의 생산방법
    • 使用HYPOCOTYL再生胎盘的方法,并生产用有用的外源基因转化的蛋白酶
    • KR1020020006263A
    • 2002-01-19
    • KR1020000039834
    • 2000-07-12
    • 대한민국(농촌진흥청장)
    • 조현석박범석진용문김호일
    • A01H4/00
    • C12N15/8274C12N15/8205C12N15/8289C12Q1/6895
    • PURPOSE: A method for regenerating cabbage using hypocotyl and a method for producing cabbage transformed with useful foreign gene are provided to establish a system which is allowed to introduce a foreign gene useful to cabbage. CONSTITUTION: The method for regenerating a cabbage comprises steps of germinating cabbage seed to prepare hypocotyl, isolating the hypocotyl, and regenerating a cabbage using the hypocotyl in the culture media containing silver nitrate(AgNO3). In the method for producing the transformed cabbage, the cabbage seed is firstly disinfected and germinated to prepare hypocotyl. And then, hypocotyl is isolated and pre-cultured. Expression vector comprising a foreign gene(e.g., Agrobacterium) is introduced into the hypocotyl, to allow hypocotyl to transform with the foreign gene. The hypocotyl is used to regenerate a transformed cabbage in the culture media containing silver nitrate, hereby transformed cabbage being produced.
    • 目的:提供使用下胚轴再生白菜的方法和用有用的外源基因转化的卷心菜生产方法,建立一种允许引入可用于白菜的外来基因的系统。 构成:用于再生白菜的方法包括以下步骤:在含有硝酸银(AgNO 3)的培养基中,使用下胚轴发芽白菜种子以制备下胚轴,分离下胚轴,并使用下胚轴再生白菜。 在生产转化白菜的方法中,首先对卷心菜种子进行消毒和发芽以制备下胚轴。 然后,下胚轴被分离并预培养。 将包含外来基因(例如农杆菌)的表达载体导入下胚轴,以允许下胚轴与外源基因一起转化。 下胚轴用于在含有硝酸银的培养基中再生转化的卷心菜,由此生产转化的白菜。
    • 9. 发明公开
    • 배추 품종 검정용 프라이머 및 이의 용도
    • 用于识别中华人民共和国人民法院的使用权及其用途
    • KR1020090005632A
    • 2009-01-14
    • KR1020070068822
    • 2007-07-09
    • 대한민국(농촌진흥청장)
    • 임선형조현석이시명우희종진용문
    • C12Q1/68
    • C12Q1/686
    • A primer pair for certificating the species of Chinese cabbage, a method for certificating the species of Chinese cabbage by using the primer pair, and a kit containing the primer pair are provided to allow the species of the transformed Chinese cabbage to be certificated by PCR. A primer pair for certificating the species of Chinese cabbage is selected from the group consisting of a primer pair represented by the sequences 1 and 2; a primer pair represented by the sequences 1 and 3; and a primer pair represented by the sequences 1 and 4. A method for certificating the species of Chinese cabbage comprises the steps of extracting genome DNA from Chinese cabbage; carrying out PCR by using the primer pair with a template of the extracted genome DNA; and separating the PCR product by size.
    • 提供大白菜品种鉴定的引物对,使用引物对鉴定大白菜品种的方法,以及含有引物对的试剂盒,通过PCR验证转化大白菜的种类。 用于鉴定大白菜种类的引物对选自由序列1和2表示的引物对; 由序列1和3表示的引物对; 以及由序列1和4表示的引物对。用于认证大白菜种的方法包括从大白菜中提取基因组DNA的步骤; 通过使用引物对与提取的基因组DNA的模板进行PCR; 并按照大小分离PCR产物。