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    • 2. 发明公开
    • 아그로박테리움을 이용한 배추의 형질전환 효율을 증가시키는 방법
    • 使用方法生产农作物和中国农业植物提高中国农村植物转化效率的方法
    • KR1020110093164A
    • 2011-08-18
    • KR1020100013042
    • 2010-02-11
    • 대한민국(농촌진흥청장)
    • 김도선곽정호안율균
    • A01H5/00C12N15/82
    • C12N15/8205A01H4/001A01H4/005C12N5/0025C12N15/8216C12N15/8241
    • PURPOSE: A method for enhancing transformation efficiency of Brassica campestris L. using Agrobacterium is provided to establish stable transformation system of high efficiency. CONSTITUTION: A method for enhancing transformation efficiency of Brassica campestris L. comprises: a step of performing dark germination of Brassica campestris L. seeds at 24-26°C for 3-5 days; a step of culturing the hypocotyl part of Brassica campestris L. in a MS medium containing 0.5-1.5 mg/L of NAA and 3-5 mg/L of BA at 22-24°C for 1-3 days by photo-cultivation; a step of suspending Agrobacterium tumefaciens containing a recombinanat plant expression vector to a medium; a step of dipping the hypocotyl part of Brassica campestris L. in the prepared strain-inoculated medium for 5-15 minutes; a step of culturing the hypocotyl part of Brassica campestris L. in a medium I containing NAA, BA, AgNO_3, and antibiotics for 2-3 months to induce a shoot; and a step of culturing the shoot in a medium II.
    • 目的:提供使用农杆菌提高芥菜转化效率的方法,建立高效率的稳定转化体系。 构成:提高芥菜转化效率的方法包括:在24-26℃下进行芸苔属种子黑萌发3-5天的步骤; 在含有0.5-1.5mg / L的NAA和3-5mg / L BA的MS培养基中培养芥菜的下胚轴部分在22-24℃下培养1-3天的步骤; 将含有重组酶植物表达载体的根癌农杆菌悬浮于培养基中的步骤; 将芥菜的下胚轴部分浸渍在制备的菌株接种培养基中5-15分钟的步骤; 在含有NAA,BA,AgNO_3和抗生素的培养基I中培养芥菜的下胚轴2-3个月以诱导芽的步骤; 并在培养基II培养苗条的步骤。
    • 7. 发明公开
    • 수경재배를 통한 뿌리혹 균주의 실시간 병원형 판별방법
    • 使用水力培养系统进行的PLASMODIOPHORA BRASSICAE RACE的实时分类方法
    • KR1020090085725A
    • 2009-08-10
    • KR1020080011467
    • 2008-02-05
    • 대한민국(농촌진흥청장)
    • 윤무경박수형곽정호
    • G01N33/48G01N33/46G01N33/00
    • A method for discriminating Plasmodiophora brassicae in a real time is provided to quickly detect the Plasmodiophora brassicae race. A method for discriminating Plasmodiophora brassicae race comprises: a step of germinating host plant and removing soil; a step of dipping the host plant in spore suspension to induce infection; and a step of discriminating Plasmodiophora brassicae race. A liquid for water culture comprises 708mg of KNO3, 590mg of Ca(NO3)2À4H2O, 246mg of MgSO4À7H2O, 115 mg of NH4H2PO4, 20mg of Fe-EDTA, 3mg of H3BO3, 2mg of MnSO4À4H2O, 0.22mg of ZnSO4À7H2O, 0.05mg of CuSO4À5H2O and 0.02mg of Na2MoO4À2H2O.
    • 提供了一种实时鉴别疟原虫的方法,用于快速检测豌豆豌豆种质。 鉴别疟原虫种的方法包括:萌发宿主植物并去除土壤的步骤; 将宿主植物浸泡在孢子悬浮液中以诱导感染的步骤; 以及歧视疟原虫种族的一个步骤。 用于水培养的液体包括708mg KNO 3,590mg Ca(NO 3)2·4H 2 O,246mg MgSO 4·7H 2 O,115mg NH 4 H 2 PO 4,20mg Fe-EDTA,3mg H 3 BO 3,2mg MnSO 4·4H 2 O,0.22mg ZnSO 4·7H 2 O,0.05mg CuSO 4·5H 2 O 和0.02mg Na2MoO4·2H2O。