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1. 发明公开

KR1020040022247A 수포성 구내염 바이러스의 외피당단백질 항원 및 그에대한 모노클로날 항체를 이용하는 수포성 구내염의 진단방법 有权
标题翻译：使用乙酰胆碱酯酶抗生素血管病毒和单克隆抗体的乙型脑炎病毒的诊断方法
公开(公告)号：KR1020040022247A
公开(公告)日：2004-03-12
申请号：KR1020010082714
申请日：2001-12-21
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준 , 고영준 , 이세영 , 김인중 , 주이석
IPC分类号： A61K39/12
摘要： PURPOSE: A method of diagnosing vesicular stomatitis by performing an ELISA method using the recombinant envelope glycoprotein antigen of vesicular stomatitis virus(VSV) and monoclonal antibodies thereon is provided. Therefore, it permits quick, exact and specific antibody detection of the VSV and thus exact diagnosis of vesicular stomatitis through the detection. CONSTITUTION: The diagnosing method for vesicular stomatitis is achieved through a VSV detection method consisting of: attaching monoclonal antibodies against an envelope glycoprotein antigen of VSV on a solid supporter; binding the envelope glycoprotein antigen to the monoclonal antibodies attached on the solid supporter in the first step; reacting a sample containing an antibody against the envelope glycoprotein antigen with the envelope glycoprotein antigen of the second step; reacting a secondary antibody selected from the group consisting of a conjugate antibody attached with enzyme and a conjugate antibody attached with a luminescent material with the antibody of the third step.
摘要翻译：目的：提供使用水泡性口炎病毒（VSV）的重组包膜糖蛋白抗原及其单克隆抗体进行ELISA方法诊断水泡性口炎的方法。因此，它可以快速，精确和特异性地检测VSV，从而通过检测确定水泡性口炎的诊断。构成：水泡性口炎的诊断方法是通过VSV检测方法实现的，该方法包括：将固定支持物上VSV的包膜糖蛋白抗原的单克隆抗体连接起来; 在第一步中将包膜糖蛋白抗原与附着在固体支持物上的单克隆抗体结合; 使含有针对包膜糖蛋白抗原的抗体的样品与第二步的包膜糖蛋白抗原反应; 使选自由酶附着的结合抗体和附着有发光材料的缀合物抗体的第二抗体与第三步骤的抗体反应。

2. 发明授权

KR100331177B1 돼지 유행성 설사병 바이러스의 진단 방법 및 진단키트 有权
标题翻译：一种诊断猪流行性腹泻病毒的方法和一种诊断试剂盒
公开(公告)号：KR100331177B1
公开(公告)日：2002-04-06
申请号：KR1019990025161
申请日：1999-06-29
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준 , 우승룡 , 김종만 , 조준형
IPC分类号： C12Q1/70
摘要： 본발명은돼지유행성설사병바이러스의진단방법및 진단키트에관한것으로서, 보다상세하게는돼지전염성설사질병의원인체인돼지유행성설사병바이러스항체와면역학적으로결합성이있는시료내의돼지유행성설사병바이러스항원의존재를경제적이며간편하게진단할수 있는방법및 그에유용한진단키트에관한것이다.

3. 发明公开

KR1020030052688A 아프리카 마역 바이러스의 ＶＰ7 항원 및 그에 대한모노클로날 항체를 이용하는 아프리카 마역의 진단방법 有权
标题翻译：非洲人痘痘病毒（AHSV）的VP7抗原和使用其单克隆抗体的非洲躁狂症（AHS）的诊断方法
公开(公告)号：KR1020030052688A
公开(公告)日：2003-06-27
申请号：KR1020010082715
申请日：2001-12-21
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준 , 고영준 , 이세영 , 주이석
IPC分类号： G01N33/68
摘要： PURPOSE: A VP7 antigen of African horse sickness virus(AHSV) and a diagnostic method of African horse sickness(AHS) using a monoclonal antibody therefor are provided, thereby rapidly and accurately diagnosing AHSV. CONSTITUTION: A diagnostic method of African horse sickness(AHS) using a monoclonal antibody to VP7 antigen of African horse sickness virus(AHSV) comprises the steps of: (1) adhering a monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) to a solid support; (2) binding the VP7 antigen of AHSV with the monoclonal antibody adhered to the solid support; (3) reacting a sample containing an antibody for a VP7 protein with the VP7 antigen of AHSV; (4) reacting a secondary antibody selected from the group consisting of an enzyme conjugated antibody, a radioactive material conjugated antibody and a fluorescent material conjugated antibody, with the antibody of the step 3; and (5) quantifying the antibody in the sample, wherein the monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) is produced from a hybridoma cell(KCTC 10136BP).
摘要翻译：目的：提供非洲马病病毒（AHSV）的VP7抗原和使用其单克隆抗体的非洲马病诊断方法（AHS），从而快速准确地诊断AHSV。构成：使用非洲马病病毒（AHSV）的VP7抗原单克隆抗体的非洲马病诊断方法（AHS）包括以下步骤：（1）将非洲马病病毒（AHSV）VP7抗原的单克隆抗体坚定的支持; （2）将AHSV的VP7抗原与附着在固体支持物上的单克隆抗体结合; （3）将含有VP7蛋白抗体的样品与AHSV的VP7抗原反应; （4）将选自酶结合抗体，放射性物质缀合抗体和荧光物质缀合抗体的二抗与步骤3的抗体反应; 和（5）定量样品中的抗体，其中非洲马病病毒（AHSV）的VP7抗原单克隆抗体由杂交瘤细胞（KCTC 10136BP）产生。

4. 发明授权

KR100267748B1 돼지 전염성 코로나바이러스의 정제방법 有权
标题翻译： PORCINE可传染性冠状病毒的方法
公开(公告)号：KR100267748B1
公开(公告)日：2000-10-16
申请号：KR1019980017349
申请日：1998-05-14
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준
IPC分类号： C12N7/02
摘要： PURPOSE: A method for purifying porcine infectious coronavirus is provided, thereby the porcine infectious coronavirus can be simply and rapidly purified without losing its activity, so that it can be used in producing vaccines or diagnosis kits. CONSTITUTION: The method for purifying porcine infectious coronavirus comprises the steps of: packing the column with crosslinked polysaccharides or sulfate ester of cellulose; washing the column with TEN buffer solution(pH 7.5) consisting of 10mM of Tris, 1mM of EDTA and 100mM of sodium chloride; homogenizing the column with TEN buffer solution; passing the porcine infectious coronavirus containing solution through the column to adsorb it to the column; washing the column with TEN buffer solution; and eluting the porcine infectious coronavirus with TEN buffer solution(pH 7.5) consisting of 10mM of Tris, 1mM of EDTA and 1.0 to 3.0M of sodium chloride.
摘要翻译：目的：提供猪传染性冠状病毒的纯化方法，可以简单，快速地纯化猪感染性冠状病毒，而不会失去活性，可用于生产疫苗或诊断试剂盒。构成：猪传染性冠状病毒的纯化方法包括以下步骤：用交联的多糖或纤维素硫酸酯包装柱; 用10mM Tris，1mM EDTA和100mM氯化钠组成的TEN缓冲溶液（pH7.5）洗涤柱子; 用TEN缓冲溶液均质柱; 将含有感染性冠状病毒的溶液通过柱吸附到柱上; 用TEN缓冲溶液洗涤柱; 并用10mM Tris，1mM EDTA和1.0-3.0M氯化钠组成的TEN缓冲溶液（pH7.5）洗脱猪感染性冠状病毒。

5. 发明公开

KR1020010037612A 돼지 대장균 설사증 예방 및 치료용 특이항체를 포함한 난황건조분말을 이용한 사료첨가제 无效
标题翻译：饲料添加剂使用具有防腐和治疗猪苓蓉的干酪粉
公开(公告)号：KR1020010037612A
公开(公告)日：2001-05-15
申请号：KR1019990045242
申请日：1999-10-19
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 우승룡 , 김종만 , 이지연 , 권창희 , 오진식 , 권병준 , 김종염
IPC分类号： A23K1/18
CPC分类号： A23K50/00 , A23K10/20 , A23K40/10
摘要： PURPOSE: A feed additive is provided to prevent and treat the pig colon bacillus diarrhea by using dried yolk powder having an idioantibody. CONSTITUTION: A testing vaccine manufactured by using a cilium antigen of the colon bacillus is inoculated on a layer and immunized. The eggs are disinfected with alcohol and the egg yolk is aseptically collected in an Erlenmeyer Flask. The collected yolk is injected into a powder dryer to gather yolk powder. The injecting temperature of the powder dryer is 140deg.C and the releasing temperature is 72deg.C. The dried powder weight per 1ml vol and the moisture content of the egg yolk are 0.38g and 2%, respectively. The value of an antibody is rarely changed before and after the drying.
摘要翻译：目的：提供饲料添加剂，通过使用具有不良反应的干燥的蛋黄粉来预防和治疗猪大肠杆菌腹泻。构成：将通过使用大肠杆菌的纤毛抗原制造的测试疫苗接种在一层上并免疫。鸡蛋用酒精消毒，蛋黄被无菌地收集在锥形瓶中。将收集的蛋黄注入粉末干燥器中以收集蛋黄粉末。粉末干燥机的注射温度为140℃，脱模温度为72℃。每1ml体积的干燥粉末重量和蛋黄的水分含量分别为0.38g和2％。干燥前后抗体的值很少变化。

6. 发明授权

KR100449908B1 수포성 구내염 바이러스의 외피당단백질 항원 및 그에대한 모노클로날 항체를 이용하는 수포성 구내염의 진단방법 有权
标题翻译： 수포성구내염바이러스의외피당단백질항원및그에대한모노클로날항체를이용하는수포성구내염의진단방
公开(公告)号：KR100449908B1
公开(公告)日：2004-09-22
申请号：KR1020010082714
申请日：2001-12-21
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준 , 고영준 , 이세영 , 김인중 , 주이석
IPC分类号： A61K39/12
摘要： PURPOSE: A method of diagnosing vesicular stomatitis by performing an ELISA method using the recombinant envelope glycoprotein antigen of vesicular stomatitis virus(VSV) and monoclonal antibodies thereon is provided. Therefore, it permits quick, exact and specific antibody detection of the VSV and thus exact diagnosis of vesicular stomatitis through the detection. CONSTITUTION: The diagnosing method for vesicular stomatitis is achieved through a VSV detection method consisting of: attaching monoclonal antibodies against an envelope glycoprotein antigen of VSV on a solid supporter; binding the envelope glycoprotein antigen to the monoclonal antibodies attached on the solid supporter in the first step; reacting a sample containing an antibody against the envelope glycoprotein antigen with the envelope glycoprotein antigen of the second step; reacting a secondary antibody selected from the group consisting of a conjugate antibody attached with enzyme and a conjugate antibody attached with a luminescent material with the antibody of the third step.
摘要翻译：目的：提供一种使用水疱性口炎病毒（VSV）的重组包膜糖蛋白抗原和单克隆抗体进行ELISA方法来诊断水疱性口炎的方法。因此，它可以快速，准确和特异地检测VSV的抗体，从而通过检测来精确诊断水疱性口炎。组成：水泡性口炎的诊断方法通过VSV检测方法实现，该方法包括：将抗VSV的包膜糖蛋白抗原的单克隆抗体附着在固体支持物上; 在第一步中将包膜糖蛋白抗原结合至附着于固体载体上的单克隆抗体; 使含有抗包膜糖蛋白抗原的抗体的样品与第二步的包膜糖蛋白抗原反应; 使选自附着有酶的缀合物抗体和附着有荧光物质的缀合物抗体的第二抗体与第三步骤的抗体反应。

7. 发明授权

KR100430935B1 대장균 발현 재조합 3ＡＢＣ 비구조단백질 및단클론항체를 이용한 구제역 진단방법 有权
标题翻译： 3ABC비장균발현재조비단백질단백질단클론항를를이용한구제역진단방
公开(公告)号：KR100430935B1
公开(公告)日：2004-05-14
申请号：KR1020010082975
申请日：2001-12-21
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준 , 고영준 , 나진주 , 김원일 , 이세영 , 주이석
IPC分类号： C12N15/33
摘要： PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
摘要翻译：目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫诊断方法，从而比现有方法更快速和更准确地诊断口蹄疫。构成：编码来源于韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组3ABC非结构蛋白由重组用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E.coli中表达的重组3ABC非结构蛋白制备杂交瘤细胞系3F-11（KCTC 10138BP）将大肠杆菌导入动物体内，从动物体内收集免疫细胞，并将免疫细胞与癌细胞融合。从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。口蹄疫诊断方法包括以下步骤：（1）将重组3ABC非结构蛋白稀释于包被缓冲液中，并将稀释液倒入板中; （2）使测试血清与平板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，与偶联物的荧光反应或辐射反应。

8. 发明公开

KR1020030052857A 대장균 발현 재조합 3ＡＢＣ 비구조단백질 및단클론항체를 이용한 구제역 진단방법 有权
标题翻译：使用在大肠杆菌和单克隆抗体中表达的重组体3ABC非结构蛋白的FOOT-AND-MOUTH DISEASE的诊断方法
公开(公告)号：KR1020030052857A
公开(公告)日：2003-06-27
申请号：KR1020010082975
申请日：2001-12-21
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준 , 고영준 , 나진주 , 김원일 , 이세영 , 주이석
IPC分类号： C12N15/33
CPC分类号： C07K14/005 , C07K16/1009 , C07K2317/14 , C12N5/163 , C12N15/70 , C12N2770/32131 , G01N33/56983 , G01N33/577 , G01N2333/09
摘要： PURPOSE: Diagnostic methods of foot-and-mouth disease using a recombinant 3ABC non-structural protein expressed in E. coli and a monoclonal antibody is provided, thereby more rapidly and accurately diagnosing the foot-and-mouth disease than the prior methods. CONSTITUTION: A gene encoding a recombinant 3ABC non-structural protein derived from Korean foot-and-mouth disease virus O/SKR/2000 has the nucleotide sequence of SEQ ID NO: 1. The recombinant 3ABC non-structural protein is expressed from a recombinant E. coli transformed with a recombinant vector containing the recombinant 3ABC non-structural protein gene of SEQ ID NO: 1. A hybridoma cell line 3F-11(KCTC 10138BP) is prepared by introducing the recombinant 3ABC non-structural protein expressed in E. coli into an animal, collecting an immunized cell from the animal and fusing the immunized cell with a cancer cell. A monoclonal antibody is produced from the hybridoma cell line 3F-11(KCTC 10138BP). A diagnostic method of foot-and-mouth disease comprises the steps of: (1) diluting the recombinant 3ABC non-structural protein in a coating buffer solution and pouring the dilution on a plate; (2) reacting the testing serum with the plate; (3) reacting the 3ABC non-structural protein specific monoclonal antibody with the serum; (4) reacting a 3ABC non-structural protein specific monoclonal antibody binding conjugate with the monoclonal antibody; and (5) measuring intensity of the enzyme reaction, fluorescence reaction or radiation reaction with the conjugate.
摘要翻译：目的：提供使用在大肠杆菌中表达的重组3ABC非结构蛋白和单克隆抗体的口蹄疫的诊断方法，从而比现有方法更快速和准确地诊断口蹄疫。构成：编码来自韩国口蹄疫病毒O / SKR / 2000的重组3ABC非结构蛋白的基因具有SEQ ID NO：1的核苷酸序列。重组体3ABC非结构蛋白由重组体用含有SEQ ID NO：1的重组3ABC非结构蛋白基因的重组载体转化大肠杆菌。通过引入在E中表达的重组3ABC非结构蛋白来制备杂交瘤细胞系3F-11（KCTC 10138BP）。将大肠杆菌转化成动物，从动物中收集免疫的细胞并将免疫的细胞与癌细胞融合。从杂交瘤细胞系3F-11（KCTC 10138BP）产生单克隆抗体。口蹄疫的诊断方法包括以下步骤：（1）在包被缓冲溶液中稀释重组体3ABC非结构蛋白，并将稀释液倒在平板上; （2）使测试血清与板反应; （3）使3ABC非结构蛋白特异性单克隆抗体与血清反应; （4）使3ABC非结构蛋白特异性单克隆抗体结合缀合物与单克隆抗体反应; 和（5）测量酶反应的强度，荧光反应或与缀合物的辐射反应。

9. 发明公开

KR1019990085154A 돼지 전염성 코로나바이러스의 정제방법 有权
标题翻译：猪传染性冠状病毒的纯化方法
公开(公告)号：KR1019990085154A
公开(公告)日：1999-12-06
申请号：KR1019980017349
申请日：1998-05-14
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준
IPC分类号： C12N7/02
摘要： 본 발명은 주요 돼지 전염성 질병의 원인체로 돼지 코로나바이러스군인 유행성 설사병 바이러스, 전염성 위장염 바이러스 및 호흡기 코로나바이러스를 신속하고, 간편하게 고역가로 정제하는 방법에 관한 것으로, 본 발명에 의해 정제된 바이러스는 면역제제 작성이나 ELISA법, Dip-stick법, dot blot법 등에 적용되어 바이러스의 진단에 이용될 수 있으며, 고효능의 예방약 생산 및 야외에서 손쉽게 적용할 수 있는 진단키트의 생산에도 사용될 수 있다.

10. 发明授权

KR100522086B1 아프리카 마역 바이러스의 ＶＰ7 항원 및 그에 대한모노클로날 항체를 이용하는 아프리카 마역의 진단방법 有权
标题翻译：使用非洲马蹄病病毒和单克隆抗体的VP7抗原诊断非洲马蹄病的方法
公开(公告)号：KR100522086B1
公开(公告)日：2005-11-09
申请号：KR1020010082715
申请日：2001-12-21
申请人： 대한민국(농림축산식품부 농림축산검역본부장)
发明人： 권창희 , 권병준 , 고영준 , 이세영 , 주이석
IPC分类号： G01N33/68
摘要： 본 발명은 아프리카 마역 바이러스를 검출하여, 아프리카 마역을 진단하는 방법에 관한 것으로, 더욱 상세하게는 아프리카 마역바이러스에 감염된 혈청 내에 존재하는 VP7에 대한 항체를 아프리카 마역바이러스의 VP7 항원 및 상기 VP7 항원에 대한 모노클로날 항체를 이용하는 ELISA법으로 아프리카 마역 항체를 검출함으로써, 아프리카 마역을 진단하는 방법에 관한 것이다.

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