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    • 5. 发明公开
    • 돼지 유행성 설사병 바이러스 중화 에피토프 및 이의 이용
    • 中毒病毒性鼻炎病毒及其用途
    • KR1020030082098A
    • 2003-10-22
    • KR1020020020644
    • 2002-04-16
    • 장용석김대혁양문식
    • 장용석양문식김대혁
    • C07K14/165
    • C07K14/005A61K39/215A61K2039/552C07K16/10C12N2770/20011
    • PURPOSE: A neutralizing epitope for porcine epidemic diarrhea virus and the use thereof are provided, thereby effectively preventing and treating the infection of porcine epidemic diarrhea virus. CONSTITUTION: A neutralizing epitope for porcine epidemic diarrhea virus(PEDV) comprises a polypeptide of SEQ ID NO: 1, wherein X22 is Asp or Gly, X 23 is Ser or Leu, X24 is Ser or Gly, X25 is Ser or Gly, X51 is Thr or Arg, X58 is Asn or Ser, X114 is Leu or Phe, and X137 is Ile or Val. A gene encoding the neutralizing epitope for porcine epidemic diarrhea virus has the nucleotide sequence of SEQ ID NO: 2. An anti-serum to PEDV is produced by injecting the PEDV epitope to an animal and collecting the anti-serum to PEDV from the immunized animal. An antibody to PEDV is isolated from the anti-serum to PEDV. A vaccine composition comprises the PEDV epitope.
    • 目的:提供猪流行性腹泻病毒的中和表位及其用途,有效预防和治疗猪流行性腹泻病毒感染。 构成:猪流行性腹泻病毒(PEDV)的中和表位包含SEQ ID NO:1的多肽,其中X22为Asp或Gly,X23为Ser或Leu,X24为Ser或Gly,X25为Ser或Gly,X51 是Thr或Arg,X58是Asn或Ser,X114是Leu或Phe,X137是Ile或Val。 编码猪流行性腹泻病毒中和表位的基因具有SEQ ID NO:2的核苷酸序列。通过将PEDV表位注射到动物并从抗体动物中收集抗血清至PEDV产生PEDV的抗血清 。 将PEDV的抗体从抗血清分离至PEDV。 疫苗组合物包含PEDV表位。
    • 7. 发明授权
    • 출판물의 지면 절감 방법
    • 减少出版物内容的方法
    • KR100487843B1
    • 2005-05-06
    • KR1020020075484
    • 2002-11-29
    • 김대혁
    • 김대혁
    • B42D1/00
    • 본 발명은 출판물의 지면 절감 방법에 관한 것이다. 종래 동화나 소설 등의 출판물에서는 그 내용을 이해하는 데 필요한 대화문(3) 외에 삽입되는 지문(4)의 분량만큼 동화나 소설류의 출판물의 지면(1)의 면수를 증가시키게 되는 문제점이 있다. 본 발명은 각 화자별 대화문과 두 번째 이후의 대화문을 각 화자별로 서로 다른 특정 글씨체로 표기함과 아울러 최초 대화문에 대한 최초 지문을 각 화자별 특정 글씨체로 삽입하고 이후 각 화자별 두 번째 이후의 대화문에 대해서는 지문을 삽입하지 않음으로써 동화나 소설 등의 내용을 이해하는 데 문제가 없으면서도 지면을 크게 절감할 수 있도록 한 것이다.
    • 8. 发明授权
    • 철을 축적하는 패리틴 단백질을 발현하는 효모
    • 以使其能够被使用
    • KR100468480B1
    • 2005-01-27
    • KR1020000070806
    • 2000-11-27
    • 김대혁장용석양문식
    • 김대혁양문식장용석
    • C12N15/81
    • PURPOSE: A yeast expressing a ferritin protein for the bioavailability of an iron is provided, thereby preventing and treating an iron deficiency of human. CONSTITUTION: The yeast expressing ferritin proteins for the bioavailability of an iron is prepared by constructing a recombinant expression vector pYETFAG-1 containing Tadpole ferritin heavy chain gene(TFH, M12120), and transforming yeast with the recombinant expression vector pYETFAG-1, wherein the yeast is Saccharomyces cerevisiae(KCTC 0853BP), and the recombinant expression vector pYETFAG-1 contains a promoter ADH2-GPD(alcohol dehydrogenase II-glyceraldehyde-3-phosphate dehydrogenase) and a ura gene as a selection maker other than TFH gene.
    • 目的:提供表达铁生物利用度的铁蛋白蛋白质的酵母,从而预防和治疗人体缺铁症。 构成:通过构建含有蝌蚪铁蛋白重链基因(TFH,M12120)的重组表达载体pYETFAG-1并用重组表达载体pYETFAG-1转化酵母来制备表达铁生物利用度的酵母表达铁蛋白蛋白质, 酵母是酿酒酵母(KCTC 0853BP),重组表达载体pYETFAG-1含有启动子ADH2-GPD(醇脱氢酶II-甘油醛-3-磷酸脱氢酶)和作为选择标记的ura基因。
    • 9. 发明公开
    • 출판물의 지면 절감 방법
    • 减少出版物空间的方法
    • KR1020040047323A
    • 2004-06-05
    • KR1020020075484
    • 2002-11-29
    • 김대혁
    • 김대혁
    • B42D1/00
    • PURPOSE: A method of reducing space in a publication is provided to include the same text contents and reduce the space in the publication of children's story or novel, and to reduce a time required for reading or mounting. CONSTITUTION: The method of reducing space in a publication which describes dialogue sentences of different speakers and context sentences corresponding to the dialogue sentences on the space, is characterized in that first dialogue sentences of different speakers and the second dialogue sentences or more are described in different and specific styles of characters by the speakers; only first context sentences corresponding to the first dialogue sentences by the speakers are inserted in the specified styles of characters as assigned to the dialogue sentences of the respective speakers; and no context sentences are inserted with respect to the second context sentences or more of the respective speakers.
    • 目的:提供减少出版物空间的方法,包括相同的文本内容,减少出版儿童故事或小说的空间,减少阅读或安装所需的时间。 构成:描述对应于空间上的对话句子的​​不同说话者和上下文句子的对话句的出版物中的空间的方法,其特征在于,不同说话者的第一对话句和第二对话句或更多的描述在不同的 和扬声器的特定风格的人物; 只有扬声器对应于第一对话句子的​​第一上下文句子被插入到分配给相应扬声器的对话句子的​​指定样式的字符中; 并且相对于第二上下文句子或相应的说话者的更多者,不插入上下文句子。
    • 10. 发明授权
    • 효모 발현 시스템을 이용한 인터루킨-18 단백질의생산방법
    • 효모발현시스템을이용소인터루킨-18단백질의생산방효
    • KR100468482B1
    • 2005-01-27
    • KR1020020019806
    • 2002-04-11
    • 양문식장용석김대혁
    • 양문식김대혁장용석
    • C12N15/81
    • PURPOSE: A production method of interleukin-18 using a yeast expression system is provided, thereby mass producing extracellular cytokine which has higher biological activity than cytokine expressed from a microorganism. CONSTITUTION: A vector comprises (a) GPD(glyceraldehyde-3-phosphate dehydrogenase) promoter(GenBank accession number M13807) or AG promoter(GenBank accession number J01314); (b) extracellular secretion signal sequence; (c) cytokine gene; and (d) transcription termination signal sequence, wherein the extracellular secretion signal sequence is amylase 1A secretion signal sequence (Ramy1A, GenBank accession number X16509); the cytokine gene is a gene encoding interleukin-18 mature protein; the vector is pYEGIL18 or pYEAGIL18. A production method of interleukin-18 comprises the steps of: (a) inserting a heterogenous gene into a vector comprising GPD promoter or AG promoter, extracellular secretion signal sequence, cytokine gene and transcription termination signal sequence to prepare a recombinant vector; (b) transforming yeast with the recombinant vector; and (c) culturing the transformed yeast in a medium.
    • 目的:提供一种使用酵母表达系统的白细胞介素-18的生产方法,由此大量生产具有比从微生物表达的细胞因子更高生物活性的细胞外细胞因子。 组成:载体包含(a)GPD(甘油醛-3-磷酸脱氢酶)启动子(GenBank登录号M13807)或AG启动子(GenBank登录号J01314); (b)细胞外分泌信号序列; (c)细胞因子基因; 和(d)转录终止信号序列,其中细胞外分泌信号序列是淀粉酶1A分泌信号序列(Ramy1A,GenBank登录号X16509); 细胞因子基因是编码白细胞介素-18成熟蛋白的基因; 载体是pYEGIL18或pYEAGIL18。 白介素-18的生产方法包括以下步骤:(a)将异源基因插入包含GPD启动子或AG启动子,细胞外分泌信号序列,细胞因子基因和转录终止信号序列的载体中以制备重组载体; (b)用重组载体转化酵母; 和(c)在培养基中培养转化的酵母。