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1. 发明专利

JP2015006209A 無オリゴペプチド細胞培地审中-公开
标题翻译：无细胞免疫细胞培养基
公开(公告)号：JP2015006209A
公开(公告)日：2015-01-15
申请号：JP2014210677
申请日：2014-10-15
申请人：バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , Baxter Internatl Inc , バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare SA , Baxter Healthcare Sa , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare SA
发明人： GRILLBERGER LEOPOLD , REITER MANFRED , WOLFGANG MUNDT , ARTUR MITTERER
IPC分类号： C12N1/00 , C12N1/16 , C12N1/20 , C12N5/071 , C12N7/02 , C12N15/09
CPC分类号： C07K14/755 , C12N5/0018 , C12N5/005 , C12N7/00 , C12N2500/05 , C12N2500/10 , C12N2500/20 , C12N2500/24 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2710/24151
摘要：【課題】無オリゴペプチド細胞培地を提供すること。【解決手段】本発明は、ポリアミンを少なくとも0.5mg/L含む無オリゴペプチド細胞培地およびポリアミンを少なくとも0.5mg/L含む無オリゴペプチド細胞培地で細胞を培養する方法に関する。本発明は、また、ポリアミンを少なくとも0.5mg/L含む培地で少なくとも1つのタンパク質を発現させる方法およびポリアミンを少なくとも0.5mg/L含む培地で少なくとも1つのウイルスを生成する方法に関する。さらに、本発明による無オリゴペプチド培地は、着実な細胞増殖および所望の産物、特に、組換えタンパク質および/またはウイルス等の標的タンパク質の収率の上昇を可能にし、どのようなタンパク質加水分解物の品質またはロット差異にも依存しない。ポリアミンの特異的濃度による細胞培地の特異的補充は、細胞増殖、細胞の特異的生産性、および最終的な細胞密度を増加させるように作用する。【選択図】なし
摘要翻译：要解决的问题：提供无寡肽的细胞培养基。解决方案：本发明涉及包含至少0.5mg / L多胺的无寡肽的细胞培养基和用于在无寡肽的细胞培养物中培养细胞的方法培养基包含至少0.5mg / L的多胺。本发明还涉及用于在包含至少0.5mg / L多胺的培养基中表达至少一种蛋白质的方法和用于在包含至少0.5mg / L多胺的培养基中产生至少一种病毒的方法。此外，根据本发明的无寡肽培养基允许一致的细胞生长和所需产物，特别是靶蛋白如重组蛋白和/或病毒的产量增加，与任何蛋白质水解产物的质量或批次变化无关。具有特定浓度多胺的细胞培养基的特异性补充作用是增加细胞生长，细胞比生产力和最终细胞密度。

2. 发明专利

JP2014061005A Cell culture medium for adamts protein expression 有权
标题翻译：细胞培养基用于ADAMTS蛋白表达
公开(公告)号：JP2014061005A
公开(公告)日：2014-04-10
申请号：JP2013269216
申请日：2013-12-26
申请人： Baxter Internatl Inc , バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , Baxter Healthcare Sa , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare SA
发明人： GRILLBERGER LEOPOLD , ALEXANDRA SPENGER , MEINHARD HASSLACHER , RANA GRILLBERGER , LEITER MANFRED
IPC分类号： C12N9/50 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/02
CPC分类号： C12N9/6489 , C12N5/0682 , C12N5/0686 , C12N2500/14 , C12N2500/22 , C12N2500/38 , C12N2500/95 , C12N2500/99 , C12N2510/02 , C12Y304/24087
摘要： PROBLEM TO BE SOLVED: To provide a cell culture medium for ADAMTS protein expression.SOLUTION: The present invention provides culture mediums that are useful for expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the present invention are useful for expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein.
摘要翻译：要解决的问题：提供用于ADAMTS蛋白表达的细胞培养基。解决方案：本发明提供可用于表达ADAMTS蛋白质的ADAMTS13的培养基。还提供了ADAMTS蛋白的表达和纯化方法。在一些实施方案中，本发明的培养基和方法可用于表达具有高比活性的ADAMTS蛋白。还提供了ADAMTS，例如ADAMTS13，具有高比活性的蛋白质组合物，其根据本文提供的方法表达和纯化。

3. 发明专利

JP2011041580A Animal protein-free media for cultivation of cell 有权
标题翻译：用于培养细胞的动物无蛋白质培养基
公开(公告)号：JP2011041580A
公开(公告)日：2011-03-03
申请号：JP2010265835
申请日：2010-11-29
申请人： Baxter Healthcare Sa , Baxter Internatl Inc , バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare S.A.
发明人： LEITER MANFRED , MUNDT WOLFGANG , GRILLBERGER LEOPOLD , KRAUS BARBARA
IPC分类号： C12N1/00 , A61K39/12 , A61K39/145 , A61K39/275 , A61K39/285 , A61P37/02 , C12N5/00 , C12N5/02 , C12N5/06 , C12N5/10 , C12N7/00
CPC分类号： C12N5/005 , A61K39/12 , A61K39/145 , A61K39/275 , A61K39/285 , C12N5/0043 , C12N7/00 , C12N2500/74 , C12N2500/76 , C12N2710/24134 , C12N2760/16134 , C12N2770/36134
摘要： PROBLEM TO BE SOLVED: To provide animal protein-free cell culture media.
SOLUTION: The animal protein-free cell culture media comprises a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein-free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production biological products by cell culture processes under conditions devoid of animal protein components.
COPYRIGHT: (C)2011,JPO&INPIT
摘要翻译：要解决的问题：提供无动物蛋白的细胞培养基。解决方案：无动物蛋白质的细胞培养基包含衍生自大豆水解产物和酵母水解产物的非动物衍生肽的组合。本发明还提供了一种无动物蛋白质的培养方法，其中培养，繁殖和传代细胞而没有动物衍生的成分。该方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及在没有动物蛋白质组分的条件下通过细胞培养方法生产生物产物。版权所有（C）2011，JPO＆INPIT

4. 发明专利

JP2012034712A Animal protein-free medium for cultivation of cell 有权
标题翻译：用于培养细胞的动物蛋白质免费媒体
公开(公告)号：JP2012034712A
公开(公告)日：2012-02-23
申请号：JP2011258106
申请日：2011-11-25
申请人： Baxter Healthcare Sa , Baxter Internatl Inc , バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare S.A.
发明人： LEITER MANFRED , MUNDT WOLFGANG , GRILLBERGER LEOPOLD , KRAUS BARBARA
IPC分类号： C12N1/00 , A61K39/12 , A61K39/145 , A61K39/275 , A61K39/285 , A61P37/02 , C07K4/02 , C12N5/00 , C12N5/02 , C12N5/06 , C12N5/10 , C12N7/00 , C12N7/02
CPC分类号： C12N5/005 , A61K39/12 , A61K39/145 , A61K39/275 , A61K39/285 , C12N5/0043 , C12N7/00 , C12N2500/74 , C12N2500/76 , C12N2710/24134 , C12N2760/16134 , C12N2770/36134
摘要： PROBLEM TO BE SOLVED: To provide animal protein-free media for cultivation of cells.SOLUTION: The present invention relates to animal protein-free cell culture media comprising a combination of non-animal-derived peptides derived from soy hydrolyzate and yeast hydrolyzate. The invention also provides an animal protein-free culture process, wherein cells are cultivated, propagated, and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes under conditions devoid of animal protein components.
摘要翻译：待解决的问题：提供用于培养细胞的无动物蛋白质的培养基。解决方案：本发明涉及含有源自大豆水解产物和酵母水解产物的非动物衍生肽的组合的无动物蛋白质的细胞培养基。本发明还提供了一种不含动物蛋白的培养方法，其中培养，繁殖和传代细胞而没有动物衍生的成分。该方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及在没有动物蛋白质组分的条件下通过细胞培养过程生产生物产物。版权所有（C）2012，JPO＆INPIT

5. 发明专利

JP2013198502A Two-step temperature profile for propagation of virus 有权
标题翻译：用于传播病毒的两步温度剖面
公开(公告)号：JP2013198502A
公开(公告)日：2013-10-03
申请号：JP2013141293
申请日：2013-07-05
申请人： Baxter Internatl Inc , バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , Baxter Healthcare Sa , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare S.A.
发明人： REITER MANFRED , GRILLBERGER LEOPOLD , MUNDT WOLFGANG
IPC分类号： C12N7/02 , C12N7/04
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5252 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251 , C12N2770/24151 , C12N2770/36151
摘要： PROBLEM TO BE SOLVED: To provide a method for producing a virus.SOLUTION: A production method includes steps of providing a host cell that has been infected by the virus and cultivating the infected host cell at two different temperatures. The virus produced by the cultivation step is subsequently collected. By using the dual temperature cultivation process, high titer and improved purity can be obtained. For many viruses, cultivation conditions can be substantially improved by using a two temperature profile. A higher temperature is applied for a first phase of the virus propagation, which accelerates the formation of infectious virus particles. In a second phase, a lower temperature is applied to maintain the initial high titer obtained in the higher temperature propagation period and to allow the formation of stable antigen which may be used for further production of immunogenic vaccines. It is a goal to provide improved growth conditions which do not affect the quality of the produced antigens for vaccination purposes.
摘要翻译：要解决的问题：提供一种生产病毒的方法。解决方案：制备方法包括提供已被病毒感染并在两个不同温度下培养受感染宿主细胞的宿主细胞的步骤。随后收集由培养步骤产生的病毒。通过使用双温培养方法，可以获得高滴定度和改善的纯度。对于许多病毒，通过使用两个温度曲线可以显着改善培养条件。对病毒繁殖的第一阶段施加较高的温度，这加速了感染性病毒颗粒的形成。在第二阶段，施加较低的温度以维持在较高温度传播期获得的初始高效价，并允许形成可用于进一步生产免疫原性疫苗的稳定抗原。提供改善的生长条件的目的是不影响用于疫苗接种目的的产生的抗原的质量。

6. 发明专利

JP2013172741A Animal protein free medium for cultivation of cell 有权
标题翻译：用于培养细胞的动物蛋白质免费媒体
公开(公告)号：JP2013172741A
公开(公告)日：2013-09-05
申请号：JP2013100500
申请日：2013-05-10
申请人： Baxter Internatl Inc , バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , Baxter Healthcare Sa , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare S.A.
发明人： REITER MANFRED , MUNDT WOLFGANG , GRILLBERGER LEOPOLD , KRAUS BARBARA
IPC分类号： C12N1/00 , C12N7/02 , A61K39/12 , A61K39/145 , A61K39/275 , A61K39/285 , A61P37/02 , C12N5/00 , C12N5/02 , C12N5/06 , C12N5/10 , C12N7/00
CPC分类号： C12N5/005 , A61K39/12 , A61K39/145 , A61K39/275 , A61K39/285 , C12N5/0043 , C12N7/00 , C12N2500/74 , C12N2500/76 , C12N2710/24134 , C12N2760/16134 , C12N2770/36134
摘要： PROBLEM TO BE SOLVED: To provide methods of producing immunogenic compositions comprising viruses or virus antigens.SOLUTION: The present invention relates to animal protein free cell culture media comprising a combination of non-animal derived peptides derived from soy hydrolysate and yeast hydrolysate. The invention also provides an animal protein free culture process, wherein cells are cultivated, propagated and passaged without animal-derived components. This process is useful for cultivating cells, such as recombinant cells or cells infected with a virus, and for production of biological products by cell culture processes under conditions devoid of animal protein components.
摘要翻译：要解决的问题：提供产生包含病毒或病毒抗原的免疫原性组合物的方法。解决方案：本发明涉及含有源自大豆水解产物和酵母水解产物的非动物衍生肽的组合的无动物蛋白质的细胞培养基。本发明还提供了一种无动物蛋白质的培养方法，其中培养，繁殖和传代细胞而没有动物衍生的成分。该方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及在没有动物蛋白质组分的条件下通过细胞培养过程生产生物产物。

7. 发明专利

JP2013106616A Oligopeptide-free cell culture medium 审中-公开
标题翻译：无细胞免疫细胞培养液
公开(公告)号：JP2013106616A
公开(公告)日：2013-06-06
申请号：JP2013040650
申请日：2013-03-01
申请人： Baxter Internatl Inc , バクスター・インターナショナル・インコーポレイテッドBaxter International Incorp0Rated , Baxter Healthcare Sa , バクスター・ヘルスケヤー・ソシエテ・アノニムBaxter Healthcare S.A.
发明人： GRILLBERGER LEOPOLD , REITER MANFRED , MUNDT WOLFGANG , MITTERER ARTUR
IPC分类号： C12N1/00 , C12N5/10 , C12N7/00 , C12P21/02
CPC分类号： C07K14/755 , C12N5/0018 , C12N5/005 , C12N7/00 , C12N2500/05 , C12N2500/10 , C12N2500/20 , C12N2500/24 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2710/24151
摘要： PROBLEM TO BE SOLVED: To provide oligopeptide-free cell culture media.SOLUTION: The present invention relates to the oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in the oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine, and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine. Further, the oligopeptide-free medium allows consistent cell growth and increased yield of desired products, particularly of target proteins such as recombinant proteins and/or viruses, independent of the quality or lot variations of any protein hydrolysates. The specific supplementation of cell culture media with specific concentration of polyamines acts to increase cell growth, cell specific productivity and final cell density.
摘要翻译：要解决的问题：提供无寡肽的细胞培养基。解决方案：本发明涉及包含至少0.5mg / L多胺的无寡肽的细胞培养基和用于在无寡肽的细胞培养基中培养细胞的方法，该培养基包含至少0.5mg / L的多胺多胺本发明还涉及在包含至少0.5mg / L多胺的培养基中表达至少一种蛋白质的方法，以及用于在包含至少0.5mg / L多胺的培养基中产生至少一种病毒的方法。此外，无寡肽的培养基允许所需产物，特别是靶蛋白如重组蛋白和/或病毒的一致细胞生长和产量增加，而与任何蛋白质水解产物的质量或批次变化无关。特定浓度的多胺的细胞培养基的特异性补充作用是增加细胞生长，细胞比生产力和最终细胞密度。版权所有（C）2013，JPO＆INPIT

8. 发明申请

WO2008135229A2 FORMULATION OF SUGAR SOLUTIONS FOR CONTINUOUS ULTRACENTRIFUGATION FOR VIRUS PURIFICATION 审中-公开
标题翻译：用于连续超声造影用于病毒纯化的糖溶液的配方
公开(公告)号：WO2008135229A2
公开(公告)日：2008-11-13
申请号：PCT/EP2008003531
申请日：2008-04-30
申请人： BAXTER INT , BAXTER HEALTHCARE SA , REITER MANFRED , GRILLBERGER LEOPOLD , MUNDT WOLFGANG , MITTERER ARTUR , SCHAFHAUSER HORST
发明人： REITER MANFRED , GRILLBERGER LEOPOLD , MUNDT WOLFGANG , MITTERER ARTUR , SCHAFHAUSER HORST
IPC分类号： C07K14/00
CPC分类号： C12N7/00 , A61K39/00 , A61K39/12 , A61K39/145 , C12N2750/14351 , C12N2760/16134 , C12N2760/16151 , C12N2760/16152 , C12N2760/16234 , C12N2760/16251 , C12N2760/16252
摘要： The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.
摘要翻译：本发明提供了一种病毒或病毒抗原的纯化方法，包括提供病毒制剂，并通过加入两种或多种不同浓度的缓冲糖层确定的糖的梯度离心所述病毒制剂。该方法导致更高的产量，并通过增加峰池的体积来减少病毒或病毒抗原的不想要的聚集。

9. 发明申请

WO2011012725A8 METHOD OF PRODUCING A POLYPEPTIDE OR VIRUS OF INTEREST IN A CONTINUOUS CELL CULTURE 审中-公开
标题翻译：在连续细胞培养中产生多肽或病毒的方法
公开(公告)号：WO2011012725A8
公开(公告)日：2011-05-05
申请号：PCT/EP2010061191
申请日：2010-08-02
申请人： BAXTER HEALTHCARE SA , BAXTER INT , GRILLBERGER LEOPOLD , REITER MANFRED , FLEISCHHANDERL DANIEL
发明人： GRILLBERGER LEOPOLD , REITER MANFRED , FLEISCHHANDERL DANIEL
IPC分类号： C12M3/00 , C12N5/00
CPC分类号： C12N7/00 , C12N9/6489 , C12N2710/00052 , C12P21/02 , Y02P20/52
摘要： Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d-1, and a cell density of less than about 2X107 cell/mL. Also described herein is a method for producing a polypeptide and/or virus of interest in a continuous cell culture, the method comprising culturing mammalian cells expressing the polypeptide and/or virus of interest in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d-1, and a cell density of less than about 2X107 cell/mL; and recovering the polypeptide and/or virus of interest from medium of the cell culture system.
摘要翻译：本文描述了一种恒化器样连续细胞培养系统，其组合了灌注开放系统和恒化器开放系统的某些优点，以改善哺乳动物细胞例如遗传修饰细胞的培养，特别是在无血清或化学成分确定的培养基中。本文所述的连续培养系统包括在包含细胞保留装置的连续细胞培养系统中培养哺乳动物细胞，其中所述细胞培养系统具有小于约2d-1的稀释率（D），和细胞密度小于约2×10 7个细胞/ mL。本文还描述了用于在连续细胞培养物中产生感兴趣的多肽和/或病毒的方法，所述方法包括在连续细胞培养系统中培养表达感兴趣的多肽和/或病毒的哺乳动物细胞，其包含细胞保留装置其中所述细胞培养系统具有小于约2d-1的稀释率（D）和小于约2×10 7个细胞/ mL的细胞密度; 并从细胞培养系统的培养基中回收感兴趣的多肽和/或病毒。

10. 发明申请

WO2007077217A3 OLIGOPEPTIDE-FREE CELL CULTURE MEDIA 审中-公开
标题翻译：无细胞免疫细胞培养基
公开(公告)号：WO2007077217A3
公开(公告)日：2007-11-08
申请号：PCT/EP2007000027
申请日：2007-01-03
申请人： BAXTER INT , BAXTER HEALTHCARE SA , GRILLBERGER LEOPOLD , REITER MANFRED , MUNDT WOLFGANG , MITTERER ARTUR
发明人： GRILLBERGER LEOPOLD , REITER MANFRED , MUNDT WOLFGANG , MITTERER ARTUR
IPC分类号： C12N5/00
CPC分类号： C07K14/755 , C12N5/0018 , C12N5/005 , C12N7/00 , C12N2500/05 , C12N2500/10 , C12N2500/20 , C12N2500/24 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2710/24151
摘要： The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.
摘要翻译：本发明涉及包含至少0.5mg / L多胺的无寡肽的细胞培养基和用于在包含至少0.5mg / L多胺的所述不含寡肽的细胞培养基中培养细胞的方法。本发明还涉及用于在包含至少0.5mg / L多胺的培养基中表达至少一种蛋白质的方法以及在包含至少0.5mg / L多胺的培养基中产生至少一种病毒的方法。

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