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1. 发明授权

KR100367104B1 인삼의 배발생세포의 현탁배양에 의한 체세포배와 유식물체의 대량 제조방법 失效
标题翻译：现在我们正在寻找一个适合自己的方式来解决这个问题。
公开(公告)号：KR100367104B1
公开(公告)日：2003-01-15
申请号：KR1019990013865
申请日：1999-04-19
申请人： (주) 마이크로프랜츠 , 김명조 , 김재훈
发明人： 김명조 , 김재훈 , 오승철 , 모기식 , 변경록
IPC分类号： A01H4/00
摘要： PURPOSE: A mass production method of somatic embryo and seedling by suspension culturing of Panax ginseng/Embryogenic cells, and use thereof in health food or pharmaceutical preparation and ginseng seedlings for raising are provided. CONSTITUTION: A process for the mass production of Panax ginseng/Embryogenic cells comprises of: sterilizing the albumen of ginseng seed; washing with sterilized water for 3times; culturing at MS(Murashige and Skoog) agar plate; after 4weeks, compact embryogenic callus is induced; culturing the embryogenic callus at MS agar plate to get friable embryogenic callus; selecting the friable embryogenic callus, and suspension culturing at MS liquid medium for 3weeks to get large quantity of embryogenic cells. A process for the mass production of somatic embryo and seedling comprises of: culturing the embryogenic cells at MS liquid medium for 3weeks under fluorescence light to get globular shaped embryo; and culturing successively to separate somatic embryo and seedling from Panax ginseng/Embryogenic cells.
摘要翻译：目的：提供一种通过悬浮培养人参/胚胎细胞的体细胞胚胎和苗的批量生产方法，以及其在健康食品或药物制剂中的用途以及用于育苗的人参幼苗。组成：大量生产人参/胚胎发生细胞的方法包括：消毒人参种子的蛋白; 用无菌水洗涤3次; 在MS（Murashige and Skoog）琼脂平板上培养; 4周后，致密胚发生愈伤组织被诱导; 在MS琼脂平板上培养胚性愈伤组织以获得易碎的胚发生愈伤组织; 选择易碎的胚性愈伤组织，在MS液体培养基中悬浮培养3周以获得大量的胚胎发生细胞。大量生产体细胞胚和幼苗的方法包括：在MS液体培养基中在荧光下培养3周以获得球形胚; 并连续培养以从人参/胚胎细胞中分离体细胞胚和幼苗。

2. 发明公开

KR1020000066625A 인삼의 배발생세포의 현탁배양에 의한 체세포배와 유식물체의 대량 제조방법 失效
标题翻译：通过悬吊培养PANAX GINSENG /胚胎细胞进行生物体质量生产方法及其使用及其用途
公开(公告)号：KR1020000066625A
公开(公告)日：2000-11-15
申请号：KR1019990013865
申请日：1999-04-19
申请人： (주) 마이크로프랜츠 , 김명조 , 김재훈
发明人： 김명조 , 김재훈 , 오승철 , 모기식 , 변경록
IPC分类号： A01H4/00
CPC分类号： A01H4/005 , A01H4/001 , C12N5/0025
摘要： PURPOSE: A mass production method of somatic embryo and seedling by suspension culturing of Panax ginseng/Embryogenic cells, and use thereof in health food or pharmaceutical preparation and ginseng seedlings for raising are provided. CONSTITUTION: A process for the mass production of Panax ginseng/Embryogenic cells comprises of: sterilizing the albumen of ginseng seed; washing with sterilized water for 3times; culturing at MS(Murashige and Skoog) agar plate; after 4weeks, compact embryogenic callus is induced; culturing the embryogenic callus at MS agar plate to get friable embryogenic callus; selecting the friable embryogenic callus, and suspension culturing at MS liquid medium for 3weeks to get large quantity of embryogenic cells. A process for the mass production of somatic embryo and seedling comprises of: culturing the embryogenic cells at MS liquid medium for 3weeks under fluorescence light to get globular shaped embryo; and culturing successively to separate somatic embryo and seedling from Panax ginseng/Embryogenic cells.
摘要翻译：目的：提供人参/胚胎细胞的悬浮培养体细胞胚和幼苗的大规模生产方法及其在保健食品或药物制剂和人参幼苗中的应用。构成：人参/胚胎细胞大规模生产的过程包括：灭菌人参种子的白蛋白; 用无菌水洗涤3次; 在MS（Murashige和Skoog）琼脂培养基上培养; 4周后，诱导致密的胚发生愈伤组织; 在MS琼脂平板上培养胚性愈伤组织以获得易碎的胚性愈伤组织; 选择易碎的胚性愈伤组织，并在MS液体培养基上悬浮培养3周，以获得大量的胚胎发生细胞。大量生产体细胞胚和幼苗的方法包括：在荧光下在MS液体培养基上培养3周的胚胎发生细胞，得到球形胚; 并连续培养，从人参/胚胎细胞分离体细胞胚和幼苗。

3. 发明授权

KR100323571B1 가시오갈피 배발생 세포로부터 생식용 유식물체의 제조방법 및 그 추출물의 용도 失效
标题翻译：从发芽器官生产生殖油植物的过程和提取物的使用
公开(公告)号：KR100323571B1
公开(公告)日：2002-02-19
申请号：KR1019990004108
申请日：1999-02-06
申请人： (주) 마이크로프랜츠 , 김명조 , 김재훈
发明人： 김명조 , 김재훈 , 민준기 , 오승철 , 모기식 , 변경록 , 김주성 , 임요섭 , 권용수 , 김환묵 , 김창민
IPC分类号： A01H4/00
摘要： PURPOSE: A process for preparing seedlings from embryogenic cells of Eleutherococcus senticosus Max and the use of an extract thereof are provided, thereby rapidly preparing a large quantity of Eleutherococcus senticosus Max seedlings. CONSTITUTION: A process for preparing seedlings from embryogenic cells of Eleutherococcus senticosus Max comprises the steps of: culturing embryogenic cells of Eleutherococcus senticosus Max(KCTC 0504BP) in a hormone free broth medium in the dark for 10 to 20 days; filtering the cultured medium with a 150 to 300 micrometer sieve; transferring the filtrated cell pellets to the hormone free broth medium and culturing it under fluorescent light and filtering the cultured medium with a 5 to 9 mm sieve; transferring the filtrated seedlings on an MS agar medium and culturing it under fluorescent light for 5 to 9 days; selecting seedlings of Eleutherococcus senticosus Max showing excellent growth activity; and culturing them on an agar medium under fluorescent light for 5 to 9 days.

4. 发明公开

KR1019990037867A 가시오갈피 배발생 세포로부터 생식용 유식물체의 제조방법 및 그 추출물의 용도 失效
标题翻译：来自Elutheroccus senticosus最大和使用的胚胎细胞的可食用幼苗的制造和提取方法
公开(公告)号：KR1019990037867A
公开(公告)日：1999-05-25
申请号：KR1019990004108
申请日：1999-02-06
申请人： (주) 마이크로프랜츠 , 김명조 , 김재훈
发明人： 김명조 , 김재훈 , 민준기 , 오승철 , 모기식 , 변경록 , 김주성 , 임요섭 , 권용수 , 김환묵 , 김창민
IPC分类号： A01H4/00
CPC分类号： A01H4/005 , A01H4/001 , C12N5/0025
摘要： 본발명은가시오갈피(Max)의배발생세포 (/ Embryogenic cells)를 MS (Murashige and Skoog) 액체배지에서현탁배양하여체세포배발생과정(somatic embryo- genesis)을통해유식물체(seedlings또는 plantlet라고함)를대량생산하여한천배지에옮겨무균, 무공해상태의가시오갈피유식물체를생식용식품으로공급하는데있다. 또한, 가시오갈피유식물체로부터추출물을얻어항산화및 항지질과산화활성의의약품및 건강식품원료로공급하는데있다. 본발명은세계최초로가시오갈피배발생세포로부터유식물체를생산하는기술을개발하여공업적인규모로탱크배양에의해단기간에가시오갈피유식물체를대량으로생산할수 있다. 이와같이생산된가시오갈피유식물체를메탄올, 에탄올등과같은저급알코올로추출하고다시핵산, 에칠아세테이트및 부탄올등을사용하여순차적으로분획한가시오갈피유식물체추출물을얻는다. 상기의추출물은탁월한항산화활성뿐만아니라항지질과산화, 항암, 항바이러스, 항미생물등에도활성을나타내므로의약품, 식품, 음료, 차, 화장품및 신기능성건강보조식품등에유용하게사용될수 있다.

5. 发明公开

KR1020010010217A 생물반응기 배양법에 의한 가시오갈피 유식물체의 배양방법 및그 용도 无效
标题翻译：培养生物反应器中白US US ING ING ING INGS INGS INGS INGS INGS INGS INGS INGS INGS INGS INGS
公开(公告)号：KR1020010010217A
公开(公告)日：2001-02-05
申请号：KR1019990028968
申请日：1999-07-16
申请人： (주) 마이크로프랜츠
发明人： 김재훈 , 김명조 , 민준기 , 오승철 , 변경록
IPC分类号： C12N5/02
CPC分类号： C12N5/04 , A23L2/38 , A23L29/065 , A61K8/97 , A61K38/168
摘要： PURPOSE: A method for culturing seedlings of Eleutherococcus senticosus Max, which are useful as a source of health foods and medical supplies, in a bioreactor is provided. The seedlings cultured in the bioreactor show 20-40 times increased bioweight for 10 days, ans consequently it indicates that the culture in the bioreactor shortens the growth period from a somatic embryo to the seedlings. The seedlings of good quality are obtained with a high yield using the method. CONSTITUTION: The method for culturing seedlings of Eleutherococcus senticosus Max in a bioreactor equipped with an air lift device comprises the steps of : subculturing an embryogenic cell in Murashige and Skoog (MS) liquid medium aseptically to grow to homogeneous somatic embryo; transferring and culturing the somatic embryo in the air lift bioreactor; washing the seedlings with a sugar solution followed by culturing in a petridish comprising an agar medium at predetermined temperature; packaging the seedlings of Eleutherococcus senticosus Max under aseptic condition into a container and then storing in a refrigerator.
摘要翻译：目的：提供一种在生物反应器中培养作为健康食品和医疗用品来源的Eleutherococcus senticosus Max的幼苗的培养方法。在生物反应器中培养的幼苗显示出20-40倍生物量增加10天，因此表明生物反应器中的培养物缩短了从体细胞胚到幼苗的生长期。使用该方法以高产率获得质量好的幼苗。构成：在装有空气提升装置的生物反应器中培养洋葱的幼苗的方法包括以下步骤：无菌地将Murashige和Skoog（MS）液体培养基中的胚胎发生细胞传代培养成均质的体细胞胚; 在升降机生物反应器中转移和培养体细胞胚; 用糖溶液洗涤幼苗，然后在包含琼脂培养基的预定温度的培养基中培养; 将无菌条件下的Eleutherococcus migicosus Max的幼苗包装在容器中，然后储存在冰箱中。

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