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1. 发明授权

KR100462180B1 알츠하이머병 병인 유전자로 형질전환된 동물 및 그 제조방법 失效
标题翻译： 알츠하이머병병인유전자로형질전환된동물및그제조방알츠
公开(公告)号：KR100462180B1
公开(公告)日：2004-12-16
申请号：KR1020010072257
申请日：2001-11-20
申请人： (주) 디지탈바이오텍
发明人： 묵인희 , 심혜진 , 이철상
IPC分类号： C12N15/85
摘要： PURPOSE: A transgenic animal with genes relating to Alzheimer's disease and a production method thereof are provided, thereby the transgenic animal can be useful in studies on and development of medicines for Alzheimer disease. CONSTITUTION: An expression cassette for producing the transgenic animal with genes relating to Alzheimer's disease comprises (a) a mutated gene of amyloid precursor protein(APP), (b) a mutated gene of presenilin 1(PS1) and (c) a neuron specific promoter capable of regulating the said two mutated genes, wherein the promoter is neuron specific enolase(NSE); and an internal ribosomal entry site(IRES) may be inserted between the APP mutant gene and PS1 mutant gene. An expression vector containing the said expression cassette is constructed. The method for producing transgenic animal with genes relating to Alzheimer's disease comprises the steps of: constructing a vector containing NSE promoter and NSE intron 1; independently inserting a human APP mutant gene, a PS1 mutant gene and a BACE(β-site APP cleaving enzyme) gene into the vector to prepare three plasmids; isolating an insert to be inserted into an animal from the three plasmids; and injecting the insert into an abdominal cavity of an animal.
摘要翻译：目的：提供一种具有阿尔茨海默病相关基因的转基因动物及其生产方法，由此该转基因动物可用于阿尔茨海默病药物的研究和开发。（a）淀粉状蛋白前体蛋白（APP）的突变基因，（b）早老蛋白1（PS1）的突变基因和（c）神经元特异性能够调节所述两种突变基因的启动子，其中启动子是神经元特异性烯醇化酶（NSE）; 并且可以在APP突变基因和PS1突变基因之间插入内部核糖体进入位点（IRES）。构建含有所述表达盒的表达载体。生产具有阿尔茨海默病相关基因的转基因动物的方法包括以下步骤：构建含有NSE启动子和NSE内含子1的载体; 将人APP突变基因，PS1突变基因和BACE（β-位点APP切割酶）基因独立插入载体中以制备三种质粒; 从三种质粒中分离插入动物的插入物; 并将插入物注射到动物的腹腔内。

2. 发明公开

KR1020030041458A 알츠하이머병 병인 유전자로 형질전환된 동물 및 그 제조방법 失效
标题翻译：与阿尔茨海默病相关基因的转基因动物及其生产方法
公开(公告)号：KR1020030041458A
公开(公告)日：2003-05-27
申请号：KR1020010072257
申请日：2001-11-20
申请人： (주) 디지탈바이오텍
发明人： 묵인희 , 심혜진 , 이철상
IPC分类号： C12N15/85
CPC分类号： C12N15/8509 , A01K67/0275 , A01K2227/10 , A01K2227/105 , A01K2267/0312 , C12N2015/8545
摘要： PURPOSE: A transgenic animal with genes relating to Alzheimer's disease and a production method thereof are provided, thereby the transgenic animal can be useful in studies on and development of medicines for Alzheimer disease. CONSTITUTION: An expression cassette for producing the transgenic animal with genes relating to Alzheimer's disease comprises (a) a mutated gene of amyloid precursor protein(APP), (b) a mutated gene of presenilin 1(PS1) and (c) a neuron specific promoter capable of regulating the said two mutated genes, wherein the promoter is neuron specific enolase(NSE); and an internal ribosomal entry site(IRES) may be inserted between the APP mutant gene and PS1 mutant gene. An expression vector containing the said expression cassette is constructed. The method for producing transgenic animal with genes relating to Alzheimer's disease comprises the steps of: constructing a vector containing NSE promoter and NSE intron 1; independently inserting a human APP mutant gene, a PS1 mutant gene and a BACE(β-site APP cleaving enzyme) gene into the vector to prepare three plasmids; isolating an insert to be inserted into an animal from the three plasmids; and injecting the insert into an abdominal cavity of an animal.
摘要翻译：目的：提供具有与阿尔茨海默氏病有关的基因的转基因动物及其制备方法，由此转基因动物可用于阿尔茨海默氏病药物的研究和开发。构成：用于产生具有与阿尔茨海默病有关的基因的转基因动物的表达盒包括（a）淀粉样蛋白前体蛋白（APP）的突变基因，（b）早老素1（PS1）的突变基因和（c）神经元特异性能够调节所述两个突变基因的启动子，其中启动子是神经元特异性烯醇化酶（NSE）; 并且可以在APP突变基因和PS1突变基因之间插入内部核糖体进入位点（IRES）。构建含有所述表达盒的表达载体。用于产生与阿尔茨海默病有关的基因的转基因动物的方法包括以下步骤：构建含有NSE启动子和NSE内含子1的载体; 将人APP突变基因，PS1突变基因和BACE（β位点APP切割酶）基因独立插入载体中以制备三种质粒; 从三个质粒分离插入到动物中的插入物; 并将插入物注射到动物的腹腔中。

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