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2. 发明公开

KR1020040032791A 일본뇌염 바이러스의 신규한 전체-길이의 게놈 ＲＮＡ 및상기 ＪＥＶ 게놈 ＲＮＡ에 대한 감염성이 있는 ＪＥＶｃＤＮＡ 및 이것의 용도 有权
标题翻译：日本ENCEPHALITIS病毒的全长基因组RNA，其感染性病毒CDNA及其用途
公开(公告)号：KR1020040032791A
公开(公告)日：2004-04-17
申请号：KR1020030070422
申请日：2003-10-09
申请人： (주)씨아이디
发明人： 이영민 , 윤상임
IPC分类号： C12N15/40
CPC分类号： C12N7/00 , A61K39/00 , A61K48/00 , A61K2039/5256 , C07K14/005 , C12N15/86 , C12N2770/24121 , C12N2770/24122 , C12N2800/204 , C12N2840/20 , C12N2840/203 , Y02A50/39
摘要： PURPOSE: Full-length genomic RNA of Japanese encephalitis virus, infectious JEV cDNA therefrom, and the use thereof are provided. The JEV cDNA is useful for identifying JEV gene and studying molecular biological mechanism related to replication, transcription and translation of JEV. CONSTITUTION: Full-length genomic RNA of Korean type Japanese encephalitis virus comprises 95 bp of 5'-untranslated region, 10,299 bp of polypeptide coding region, and 574 bp of 3'-untranslated region, has the nucleotide sequence set forth in SEQ ID NO: 15, and contains 5'-terminal nucleotide sequence of 1AGAAGT- and 3'-terminal nucleotide sequence of -GATCT10968. Infectious JEV cDNA from the full-length genomic RNA of JEV comprises a promoter, SP6 or T7, upstream to the 5'-terminal of JEV genomic RNA, and a restriction enzyme recognition site, XhoI or XbaI, downstream to the 3'-terminal of JEV genomic RNA. A vector pBACT7/JVFLx/XbaI(KCTC 10346BP) contains T7 promoter and JEV cDNA. A vector pBACSP6/JVFLx/XbaI(KCTC 10347BP) contains SP6 promoter and JEV cDNA.
摘要翻译：目的：提供日本脑炎病毒的全长基因组RNA，感染性JEV cDNA及其用途。 JEV cDNA可用于鉴定JEV基因并研究与JEV的复制，转录和翻译相关的分子生物学机制。构成：韩国型日本脑炎病毒的全长基因组RNA包含95bp的5'-非翻译区，10,299bp的多肽编码区和574bp的3'非翻译区，其核苷酸序列如SEQ ID NO ：15，含有1AGAAGT的5'-末端核苷酸序列和-GATCT10968的3'末端核苷酸序列。来自JEV的全长基因组RNA的感染性JEV cDNA包含在JEV基因组RNA的5'末端上游的启动子SP6或T7，以及3'末端下游的限制酶识别位点XhoI或XbaI 的JEV基因组RNA。载体pBACT7 / JVFLx / XbaI（KCTC 10346BP）含有T7启动子和JEV cDNA。载体pBACSP6 / JVFLx / XbaI（KCTC 10347BP）含有SP6启动子和JEV cDNA。

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