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    • 2. 发明申请
    • MICROBIOLOGICAL PROCESS FOR THE PREPARATION OF (S,S)-N,N'-ETHYLENEDIAMINEDISUCCINIC ACID
    • 用于制备(S,S)-N,N'-乙烯基亚胺酸的酸的微生物学方法
    • WO1996036725A1
    • 1996-11-21
    • PCT/EP1996001868
    • 1996-05-04
    • CIBA SC HOLDING AGZÄHNER, HansCEBULLA, IngeborgHARDER, Michael
    • CIBA SC HOLDING AG
    • C12P13/00
    • C12P13/04Y10S435/822
    • A microbiological process for the preparation of N,N'-ethylenediaminedisuccinic acid of formula (1), by (a) precultivation of a strain of the genus Amycolatopsis orientalis on a specified nutrient medium (1st preculture), (b) inoculation of a nutrient solution of the same composition as the base of the production fermenter by the 1st preculture (2nd preculture), (c) inoculation of the base nutrient solution in the production fermenter with the 2nd preculture and incubation in accordance with a specified timetable, (d) feeding of the production fermenter in the fed-batch process with a feeding solution under aerobic conditions at a pH of from 2 to 8 and at a temperature of from 15 to 40 DEG C, wherein the base nutrient and feeding solutions are free of zinc-containing compounds, is described. The compound prepared by the process according to the invention is suitable as complexing agent with good biodegradability in detergents.
    • 通过(a)在特定的营养培养基(第一预培养物)上预培育东方双歧杆菌属的菌株,(b)接种营养物质的方法,用于制备式(1)的N,N'-乙二胺二琥珀酸的微生物过程 通过第一次预培养(第二次预培养),与生产发酵罐的基础相同组成的溶液,(c)按照规定的时间表,用第二次预培养和温育接种生产发酵罐中的基础营养液,(d) 在补充分批过程中,在需氧条件下,在2至8和15至40℃的温度下,用进料溶液进料生产发酵罐,其中基础营养物和进料溶液不含锌 - 含有化合物。 通过本发明方法制得的化合物适用于洗涤剂中具有良好生物降解性的络合剂。
    • 3. 发明申请
    • ORGANICALLY-BASED AGENT FOR REMOVING RUST FROM SURFACES, PRODUCTION AND USE OF SAID AGENT
    • 用于对有机基质除锈曲面,其生产和使用
    • WO1996009423A1
    • 1996-03-28
    • PCT/EP1995003670
    • 1995-09-18
    • KUNZ, Peter, M.ZÄHNER, HansWILLRETT, MonikaDAUSMANN, StefanPAUL, Michael
    • C23G01/24
    • C23G5/00C23G1/24
    • The invention concerns an agent, based on organic compounds obtained from micro-organisms, for removing rust from metal surfaces corroded with iron oxides. Rust is normally removed from metal articles using corrosive baths containing organic and/or inorganic acids which, owing to the concentrations used, must meet certain safety requirements and entail costly disposal procedures once the baths are depleted. Iron-absorbing products obtainable using biological methods are generally active in the neutral pH range, eliminating the salt production and contamination of the effluent associated with the neutralisation process normally required. Rust can be removed using reproducible intra- or extra-cellular products which have a high affinity with iron ions. The proposed agents are produced by culturing appropriate organisms in a suitable medium in such a way that they are stimulated to produce these agents. The articles requiring de-rusting are then treated by being dipped in or painted with the agent. Provision of appropriate supports to immobilise the articles facilitates use of the agents and permits recycling of the agent by suitable treatment.
    • 本发明提供了从金属表面除去锈的装置,包括铁氧化物,基于其来源于微生物的有机化合物。 金属部件生锈通常与酸洗液的帮助下完成。 这些包括由浓度的某些安全性要求的应用的水平满足,并且必须设置的消耗容量用尽后的有机和/或无机酸。 铁结合,可以通过生物方法获得的产品,通常起在中性pH范围内。 由否则必要中和,因此废水的污染的盐的形成被省略。 的帮助nachproduzierbarer具有对铁离子的高亲和性细胞内或细胞外产物,除锈可以实现的。 本发明通过适当的生物体的细胞的组合物的制备分别生长在合适的培养基,以使它们被激发以产生这些药剂。 到entrostenden份然后通过用该药剂或涂层与该喷雾处理。 一个更好的处理,从合适的载体的固定化介质的结果。 这样一来,资金通过适当的治疗恢复是可能的。