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1. 发明授权

US07344836B2 Method for nucleic acid hybridization using back and forth flow between a denaturation channel and a hybridization channel via a connection channel 失效
标题翻译：用于通过连接通道在变性通道和杂交通道之间来回流动的核酸杂交的方法
公开(公告)号：US07344836B2
公开(公告)日：2008-03-18
申请号：US10916504
申请日：2004-08-12
申请人： Yung-Chiang Chung , Yao-Sung Chang , Ming-Zheng Shiu
发明人： Yung-Chiang Chung , Yao-Sung Chang , Ming-Zheng Shiu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q2527/107
摘要： A method and apparatus designed for nucleic acid hybridization employs a hydrogen bond denaturation area with a higher temperature and a lower temperature nucleic acid hybridization area that is immobilized with nucleic acid probes. Nucleic acid-containing samples are introduced into the hydrogen bond denaturation area for heating curled nucleic acids in samples so that they become linear and are guided into the nucleic acid hybridization area. In this area, the nucleic acid hybridization rate can be multiplied by increasing the kinetic energy by the repeated flow of driven fluid. The nucleic acid hybridization apparatus provided by the invention contains a hydrogen bond denaturation area, a nucleic acid hybridization area, a two-way driving apparatus, and a temperature control element. The temperatures in the hydrogen bond denaturation area and the nucleic acid hybridization area can be maintained through the management of this temperature control element, and the fluid can gain needed kinetic energy through the two-way driving pump.
摘要翻译：设计用于核酸杂交的方法和装置使用具有较高温度的氢键变性区域和用核酸探针固定的较低温度的核酸杂交区域。将含有核酸的样品引入氢键变性区域，以加热样品中的卷曲核酸，使其变成线性的并被引导到核酸杂交区域。在这个区域，核酸杂交率可以通过重复流动的驱动流体增加动能来倍增。本发明提供的核酸杂交装置含有氢键变性区域，核酸杂交区域，双向驱动装置和温度控制元件。通过管理该温度控制元件可以维持氢键变性区域和核酸杂交区域的温度，并且流体可以通过双向驱动泵获得所需的动能。

2. 发明授权

US06682311B2 Pneumatic driving device for micro fluids wherein fluid pumping is governed by the control of the flow and direction of incident plural gas streams 失效
标题翻译：用于微流体的气动驱动装置，其中流体泵送通过控制流入和入射的多个气流的方向来控制
公开(公告)号：US06682311B2
公开(公告)日：2004-01-27
申请号：US10156054
申请日：2002-05-29
申请人： Yung-Chiang Chung , Te-Chun Wu , Yuan-Fong Kuo , Shr-Hau Huang
发明人： Yung-Chiang Chung , Te-Chun Wu , Yuan-Fong Kuo , Shr-Hau Huang
IPC分类号： F04F544
CPC分类号： F04F5/46 , F04B19/006 , F04F1/02 , F04F5/24
摘要： A pneumatic driving device and the associated method for micro fluids, wherein the pneumatic driving device for micro fluids is constructed by connecting a fluid pipe with a structure that is formed by two gas stream inlets, a gas stream fender, a gas stream flow path and a gas stream outlet. The suction, exclusion and stagnation for the micro fluids inside the fluid pipes can be accomplished by adjusting the flow rates of the gas streams into the device at the two gas stream inlets. In the invention several pneumatic driving devices are connected to form a single recursive pneumatic driving device by the concept of recursion, and the micro fluids inside several fluid pipes can be controlled to mix or to separate through the recursive pneumatic driving device by adjusting the flow rates of the gas streams into the device at different gas stream inlets.
摘要翻译：一种用于微流体的气动驱动装置和相关方法，其中用于微流体的气动驱动装置通过将流体管与由两个气流入口，气流挡泥板，气流流路和气流出口。可以通过调节在两个气流入口处进入装置的气流的流速来实现流体管内的微流体的抽吸，排除和停滞。在本发明中，通过递归的概念连接多个气动驱动装置以形成单个递归气动驱动装置，并且可以通过调节流量来控制几个流体管内的微流体混合或分离通过递归气动驱动装置的气流进入不同气流入口的装置。

3. 发明授权

US07273705B2 Apparatus and method for separating ribonucleic acid 失效
标题翻译：核糖核酸分离装置及方法
公开(公告)号：US07273705B2
公开(公告)日：2007-09-25
申请号：US10704707
申请日：2003-11-12
申请人： Yung-Chiang Chung
发明人： Yung-Chiang Chung
IPC分类号： C12P1/68 , C07H21/04
CPC分类号： B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2400/0463 , C12Q1/6806 , G01N1/34 , C12Q2565/629 , C12Q2527/107
摘要： An RNA separation method includes injecting a reaction solution into a reaction region which contains a covalent linking of specific RNA probe for hybridizing with the reaction solution; adjusting a moderate hybridization temperature in the reaction region; moving the remainder of the reaction solution to a transition region by using a pneumatic actuator; adding a buffer solution to the reaction region, and heating the reaction region to a moderate denature temperature by a heating apparatus; moving a waste solution which is product produced from the reaction region after denature to a waste region by using the pneumatic actuator; and moving the remainder solution back to the reaction region by the pneumatic actuator.
摘要翻译： RNA分离方法包括将反应溶液注入含有与反应溶液杂交的特异性RNA探针的共价连接的反应区域; 调节反应区域中的中等杂交温度; 通过使用气动致动器将反应溶液的剩余部分移动到过渡区域; 向反应区域加入缓冲溶液，并通过加热装置将反应区域加热至适度的变性温度; 通过使用气动执行机构，将从变性后的反应区域产生的产品的废液移动到废弃区域; 并通过气动致动器将剩余溶液移回反应区域。

4. 发明申请

US20050118647A1 Microfluidic mixer apparatus and microfluidic reactor apparatus for microfluidic processing 审中-公开
标题翻译：用于微流体加工的微流控混合装置和微流体反应器装置
公开(公告)号：US20050118647A1
公开(公告)日：2005-06-02
申请号：US10986818
申请日：2004-11-15
申请人： Yung-Chiang Chung , Ming-Shiung Jan , Ju-Hwa Lin , Shr-Hau Huang
发明人： Yung-Chiang Chung , Ming-Shiung Jan , Ju-Hwa Lin , Shr-Hau Huang
IPC分类号： B01F5/00 , B01F13/00 , B01J19/00 , G01N33/53
CPC分类号： B01F5/0068 , B01F13/0059 , B01F2215/0422 , B01F2215/0431 , B01F2215/045 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00828 , B01J2219/00889 , B01J2219/00921
摘要： A device for extracting nucleic acids includes a cover including a first opening and a second opening spaced apart from the first opening, a chip including a first terminal to communicate with the first opening and a second terminal to communicate with the second opening, and a flow channel of the chip including an inner surface onto which immobilized beads are dispersed for binding with nucleic acids in a fluid flowing in the flow channel.
摘要翻译：用于提取核酸的装置包括：包括第一开口和与第一开口间隔开的第二开口的盖，包括与第一开口连通的第一端子和与第二开口连通的第二端子的芯片，芯片的通道包括内表面，在该内表面上分散有固定的珠粒以与在流动通道中流动的流体中的核酸结合。

5. 发明授权

US06331073B1 Order-changing microfluidic mixer 有权
标题翻译：订单更换微流控搅拌机
公开(公告)号：US06331073B1
公开(公告)日：2001-12-18
申请号：US09692461
申请日：2000-10-20
申请人： Yung-Chiang Chung
发明人： Yung-Chiang Chung
IPC分类号： B01F1300
CPC分类号： B01F5/0646 , B01F13/0059 , Y10S366/02
摘要： An order-changing microfluid mixer comprises an extended microfluidic channel and a chamber structure that provides order-changing functions to microfluid when the microfluid passes through the chanter structure. The order-changing chamber has two openings connected to said microfluidic channel and divides said microfluidic channel into two sections. When a microfluid is driven from one section of the microfluidic channel to the other, the microfluid enters into the order-changing chamber and flows along a first side of the order-changing chamber, whereby portions of the microfluid positioned adjacent to the second side of the order-changing chamber remain unmoved. When a microfluid is driven from the other section of the microfluidic channel to the one section, the microfluid flows along the second side of the order-changing chamber, whereby portions of the microfluid positioned adjacent to the second side of the order-changing chamber are driven to the first terminal of the microfluidic channel, and portions of the microfluid positioned adjacent to the first side of the order-changing chamber stays unmoved. By driving the microfluid back and forth in the microfluidic channel, spatial orders of the microfluid may be changed as desired.
摘要翻译：顺序改变的微流体混合器包括延伸的微流体通道和室结构，当微流体通过流道结构时，该结构为微流体提供顺序改变功能。顺序改变室具有连接到所述微流体通道的两个开口，并将所述微流体通道分成两个部分。当微流体从微流体通道的一个部分驱动到另一个微流体时，微流体进入顺序改变室并沿顺序改变室的第一侧流动，从而将位于邻近第二侧的微流体部分订单更换室保持不动。当微流体从微流体通道的另一部分驱动到一个部分时，微流体沿顺序变换室的第二侧流动，从而与定序转换室的第二侧相邻的微流体部分是被驱动到微流体通道的第一端子，并且位于与顺序改变室的第一侧相邻的微流体的部分保持不移动。通过在微流体通道中来回驱动微流体，可以根据需要改变微流体的空间顺序。

6. 发明申请

US20050059027A1 Apparatus and method for separating ribonucleic acid 失效
标题翻译：核糖核酸分离装置及方法
公开(公告)号：US20050059027A1
公开(公告)日：2005-03-17
申请号：US10704707
申请日：2003-11-12
申请人： Yung-Chiang Chung
发明人： Yung-Chiang Chung
IPC分类号： B01L3/00 , B01L7/00 , C12M1/34 , C12Q1/68 , G01N1/34
CPC分类号： B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2400/0463 , C12Q1/6806 , G01N1/34 , C12Q2565/629 , C12Q2527/107
摘要： This present invention is concerned with a device and method for the mRNAs purification of prokaryotic cells. The device based on a microfluidic chip which comprises: (a) a reaction region including the heater apparatus and the specific rRNA or/and tRNA probes for hybridization; (b) a transition region for stocking the remainder of reaction solution; (c) a water region for discarding the waste buffer solutions; and (d) a pneumatic actuator apparatus including the air compressor for blowing airflow and controlling the process of reaction.
摘要翻译：本发明涉及原核细胞的mRNA纯化的装置和方法。基于微流体芯片的装置，其包括：（a）包括加热器装置和用于杂交的特异性rRNA或/和tRNA探针的反应区域; （b）用于储存反应溶液的其余部分的过渡区域; （c）用于废弃废物缓冲溶液的水域; 和（d）包括用于吹送气流的空气压缩机和控制反应过程的气动致动器装置。

7. 发明申请

US20050014185A1 Method and apparatus for nucleic acid hybridization 失效
标题翻译：核酸杂交的方法和装置
公开(公告)号：US20050014185A1
公开(公告)日：2005-01-20
申请号：US10916504
申请日：2004-08-12
申请人： Yung-Chiang Chung , Yao-Sung Chang , Ming-Zheng Shiu
发明人： Yung-Chiang Chung , Yao-Sung Chang , Ming-Zheng Shiu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6832 , C12Q2527/107
摘要： A method and apparatus designed for nucleic acid hybridization employs a hydrogen bond denaturation area with a higher temperature and a lower temperature nucleic acid hybridization area that is immobilized with nucleic acid probes. Nucleic acid-containing samples are introduced into the hydrogen bond denaturation area for heating curled nucleic acids in samples so that they become linear and are guided into the nucleic acid hybridization area. In this area, the nucleic acid hybridization rate can be multiplied by increasing the kinetic energy by the repeated flow of driven fluid. The nucleic acid hybridization apparatus provided by the invention contains a hydrogen bond denaturation area, a nucleic acid hybridization area, a two-way driving apparatus, and a temperature control element. The temperatures in the hydrogen bond denaturation area and the nucleic acid hybridization area can be maintained through the management of this temperature control element, and the fluid can gain needed kinetic energy through the two-way driving pump.
摘要翻译：设计用于核酸杂交的方法和装置使用具有较高温度的氢键变性区域和用核酸探针固定的较低温度的核酸杂交区域。将含有核酸的样品引入氢键变性区域，以加热样品中的卷曲核酸，使其变成线性的并被引导到核酸杂交区域。在这个区域，核酸杂交率可以通过重复流动的驱动流体增加动能来倍增。本发明提供的核酸杂交装置含有氢键变性区域，核酸杂交区域，双向驱动装置和温度控制元件。通过管理该温度控制元件可以维持氢键变性区域和核酸杂交区域的温度，并且流体可以通过双向驱动泵获得所需的动能。

8. 发明申请

US20080051572A1 Method for Extracting Nucleic Acids 审中-公开
标题翻译：提取核酸的方法
公开(公告)号：US20080051572A1
公开(公告)日：2008-02-28
申请号：US11865255
申请日：2007-10-01
申请人： Yung-Chiang CHUNG , Ming-Shiung JAN , Ju-Hwa LIN , Shr-Hau HUANG
发明人： Yung-Chiang CHUNG , Ming-Shiung JAN , Ju-Hwa LIN , Shr-Hau HUANG
IPC分类号： C07H1/06
CPC分类号： B01F5/0068 , B01F13/0059 , B01F2215/0422 , B01F2215/0431 , B01F2215/045 , B01J19/0093 , B01J2219/00783 , B01J2219/00822 , B01J2219/00828 , B01J2219/00889 , B01J2219/00921
摘要： A device for extracting nucleic acids includes a cover including a first opening and a second opening spaced apart from the first opening, a chip including a first terminal to communicate with the first opening and a second terminal to communicate with the second opening, and a flow channel of the chip including an inner surface onto which immobilized beads are dispersed for binding with nucleic acids in a fluid flowing in the flow channel.
摘要翻译：用于提取核酸的装置包括：包括第一开口和与第一开口间隔开的第二开口的盖，包括与第一开口连通的第一端子和与第二开口连通的第二端子的芯片，芯片的通道包括内表面，在该内表面上分散有固定的珠粒以与在流动通道中流动的流体中的核酸结合。

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