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1. 发明申请

US20070190579A1 Preparation method of biotinylated protein and detection method using the same 有权
标题翻译：生物素化蛋白质的制备方法及其检测方法
公开(公告)号：US20070190579A1
公开(公告)日：2007-08-16
申请号：US11643737
申请日：2006-12-21
申请人： Yaeta Endo , Tatsuya Sawasaki , Yuko Matsubara
发明人： Yaeta Endo , Tatsuya Sawasaki , Yuko Matsubara
IPC分类号： G01N33/53 , C07K14/47
CPC分类号： G01N33/532 , C07K1/13 , G01N33/543 , G01N33/58 , G01N2500/00
摘要： The present invention presents construction of a detection method requiring no step of removing free biotin during preparation of a biotinylated protein having a biotin tag, in a detection method of a substance interacting with a protein, and studied various preparation methods of the biotinylated protein. In order to solve the above-mentioned problem, the present inventor has found that in a cell-free protein synthesizing system, in particular, a wheat embryo cell-free protein synthesizing system, when biotinylation is performed during or after protein's synthesis, the biotinylation of the protein can be attained in an remarkably lower concentration of the biotin than that in the conventional biotinylation operations, and has accomplished the present invention by using the protein having the biotin tag in each detection system.
摘要翻译：本发明提出了在与蛋白质相互作用的物质的检测方法中，在制备具有生物素标签的生物素化蛋白质的过程中不需要除去游离生物素的步骤的检测方法的结构，并且研究了生物素化蛋白质的各种制备方法。为了解决上述问题，本发明人发现，在无细胞蛋白质合成系统中，特别是小麦胚细胞无蛋白质合成系统中，当在蛋白质合成期间或之后进行生物素化时，生物素化的蛋白质可以在生物素浓度显着低于常规生物素化操作中获得，并且通过在每个检测系统中使用具有生物素标签的蛋白质来完成本发明。

2. 发明授权

US07674593B2 Preparation method of biotinylated protein and detection method using the same 有权
标题翻译：生物素化蛋白质的制备方法及其检测方法
公开(公告)号：US07674593B2
公开(公告)日：2010-03-09
申请号：US11643737
申请日：2006-12-21
申请人： Yaeta Endo , Tatsuya Sawasaki , Yuko Matsubara
发明人： Yaeta Endo , Tatsuya Sawasaki , Yuko Matsubara
IPC分类号： G01N33/53
CPC分类号： G01N33/532 , C07K1/13 , G01N33/543 , G01N33/58 , G01N2500/00
摘要： The present invention presents construction of a detection method requiring no step of removing free biotin during preparation of a biotinylated protein having a biotin tag, in a detection method of a substance interacting with a protein, and studied various preparation methods of the biotinylated protein. In order to solve the above-mentioned problem, the present inventor has found that in a cell-free protein synthesizing system, in particular, a wheat embryo cell-free protein synthesizing system, when biotinylation is performed during or after protein's synthesis, the biotinylation of the protein can be attained in an remarkably lower concentration of the biotin than that in the conventional biotinylation operations, and has accomplished the present invention by using the protein having the biotin tag in each detection system.
摘要翻译：本发明提出了在与蛋白质相互作用的物质的检测方法中，在制备具有生物素标签的生物素化蛋白质的过程中不需要除去游离生物素的步骤的检测方法的结构，并且研究了生物素化蛋白质的各种制备方法。为了解决上述问题，本发明人发现，在无细胞蛋白质合成系统中，特别是小麦胚细胞无蛋白质合成系统中，当在蛋白质合成期间或之后进行生物素化时，生物素化的蛋白质可以在生物素浓度显着低于常规生物素化操作中获得，并且通过在每个检测系统中使用具有生物素标签的蛋白质来完成本发明。

3. 发明授权

US6143207A Wide-range thermistor material and method for producing it 失效
标题翻译：宽范围热敏电阻材料及其制造方法
公开(公告)号：US6143207A
公开(公告)日：2000-11-07
申请号：US68551
申请日：1998-05-18
申请人： Katsunori Yamada , Mitsuru Asai , Nobuo Kamiya , Yuko Matsubara
发明人： Katsunori Yamada , Mitsuru Asai , Nobuo Kamiya , Yuko Matsubara
IPC分类号： C04B35/117 , C04B35/488 , C04B35/593 , C04B35/645 , H01B3/00 , H01C7/00 , H01C7/02 , H01C7/04 , H01B1/08 , H01C7/10
CPC分类号： C04B35/488 , C04B35/117 , C04B35/584 , C04B35/593 , C04B35/6261 , C04B35/62625 , C04B35/62655 , C04B35/62695 , C04B35/62834 , C04B35/645 , H01B3/004 , H01C7/008 , H01C7/02 , H01C7/04 , H01C7/042 , H01C7/043 , C04B2235/3217 , C04B2235/3225 , C04B2235/3232 , C04B2235/383 , C04B2235/3834 , C04B2235/3873 , C04B2235/5427 , C04B2235/5436 , C04B2235/80 , C04B2235/96
摘要： Provided are wide-range thermistor materials with high responsibility, which have linear resistance temperature characteristics and a large rate of temperature-dependent resistance change in wide temperature range of from lower than room temperature to higher than 1000.degree. C. The thermistor material comprises an electrically-insulating ceramic matrix and second phase grains as discontinuously dispersed in the matrix, in which the second phase grains are of a semiconductive or conductive substance having a large rate of temperature-dependent resistance chance. The material may optionally contain a resistance-controlling additive of one or more of nitrides, borides, silicides, sulfides, oxides and carbide of elements, third phase grains with internal stress-relaxing ability having a lower modulus of elasticity than the matrix and the second phase grains, and a resistance stabilizer of TiO.sub.2 and/or Ti.sub.n O.sub.2n-1 (n=4 to 9).
摘要翻译： PCT No.PCT / JP97 / 03271 Sec。 371日期1998年5月18日 102（e）日期1998年5月18日PCT提交1997年9月16日PCT公布。第WO98 / 12714号公报 1998年3月26日提供广泛的热敏电阻材料，具有线性电阻温度特性，温度范围从室温到高于1000℃的宽温度范围内具有很大的温度依赖性电阻变化率。热敏电阻材料包括绝缘陶瓷基体和不连续分散在基质中的第二相晶粒，其中第二相晶粒是具有大的温度依赖性电阻机率的半导体或导电物质。该材料可以任选地包含一种或多种氮化物，硼化物，硅化物，硫化物，元素的氧化物和碳化物中的一种或多种的电阻控制添加剂，具有比基体更低的弹性模量的内应力松弛能力的第三相晶粒，第二相 TiO2和/或TinO2n-1（n = 4〜9）的电阻稳定剂。

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