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1. 发明授权

US5202427A DNA segment containing streptomycin resistance gene and being capable of controlling expression of said gene 失效
标题翻译：含有链霉素抗性基因并能够控制所述基因表达的DNA片段
公开(公告)号：US5202427A
公开(公告)日：1993-04-13
申请号：US659469
申请日：1991-02-25
申请人： Hamao Umezawa, deceased , Yoji Umezawa, heir , Yoji Umezawa, heir , Yoshiro Okamo, heir
发明人： Hamao Umezawa, deceased , Yoji Umezawa, heir , Yoji Umezawa, heir , Yoshiro Okamo, heir
IPC分类号： C12N9/12 , C12N15/76
CPC分类号： C12N15/76 , C12N9/12
摘要： A DNA segment of this invention is a DNA fragment of a length of about 3.8 kb which is obtained by excising with a restriction endonuclease Bgl II a hybrid plasmid pST 141 having a length of about 12.6 kb of Streptomyces griseus 4-1 strain (FERM P-7984, namely, FERM BP-1198) to give a Bgl II-Bgl II DNA fragment, and then excising with a restriction endonuclease Sph I the resultant Bgl II-Bgl II DNA fragment having a length of about 7.0 kb and having the restriction endonuclease sites as shown in the restriction endonuclease map of FIG. 1 . This DNA fragment is a DNA which contains a streptomycin resistance gene and contains, in the vicinity of this gene, such a DNA region possessing a function to control the expression of the streptomycin resistance gene. An insertion of this DNA fragment having a length of about 3.8 kb into a suitable actinomycetes plasmid vector can produce such a hybrid plasmid which acts reliably as a selected marker of the streptomycin resistance.
摘要翻译：本发明的DNA片段是长约3.8kb的DNA片段，其通过用限制性内切核酸酶Bgl II切割具有长度为约12.6kb的灰色链霉菌4-1菌株（FERM P）的杂交质粒pST141获得 -7984，即FERM BP-1198），得到Bgl II-Bgl II DNA片段，然后用限制性内切核酸酶SphI切割得到长度为约7.0kb并具有约束条件的所得Bgl II-Bgl II DNA片段如图1的限制性内切核酸酶图所示的内切核酸酶位点。 1。该DNA片段是含有链霉素抗性基因的DNA，该基因附近含有具有控制链霉素抗性基因表达功能的DNA区域。将具有约3.8kb长度的该DNA片段插入合适的放线菌质粒载体中可以产生这样的杂交质粒，其可靠地作为链霉素抗性的选择标记物起作用。

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