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    • 10. 发明授权
    • Method for the production of itaconic acid using aspergillus terreus solid state fermentation
    • 使用土曲霉固体发酵生产衣康酸的方法
    • US06171831B2
    • 2001-01-09
    • US09510449
    • 2000-02-22
    • Ying-Chieh TsaiMin-Chang HuangShuen-Fuh LinYuan-Chi Su
    • Ying-Chieh TsaiMin-Chang HuangShuen-Fuh LinYuan-Chi Su
    • C12P744
    • C12P7/44
    • The method to produce itaconic acid is a solid-state fermentation method. Sugarcane pressmud or peeled sugarcane pressmud is the support used to adsorb liquid medium for the production of itaconic acid by an Aspergillus terreus mutant strain. This mutant strain was derived from Aspergillus terreus ATCC 10020 by successive mutation. Beside the remaining sucrose in the sugarcane pressmud, other carbon source can be added, i.e. glucose, fructose, sucrose, or starch hydrolysate. Appropriate amounts of nitrogen source, such as ammonium nitrate, or ammonium sulfate are added. Mineral salts, such as potassium dihydrogen phosphate, magnesium sulfate, calcium sulfate, ferric chloride, zinc sulfate, and copper sulfate can be added to the medium. The suitable amount of liquid medium that can be added to the support is 4 to 6 times its dry weight for the sugarcane pressmud and 8 to 14 times its dry weight for the peeled sugarcane pressmud. The optimal pH of the medium is between 2.0-3.0. The fermentation temperature is between 30-40 degrees centigrade.
    • 衣康酸的制备方法是固态发酵法。 甘蔗压榨或去皮甘蔗压榨机是用于通过土曲霉突变体菌株吸附液体培养基以生产衣康酸的载体。 该突变菌株通过连续突变来源于土曲霉ATCC10020。 除了甘蔗压榨机中的剩余蔗糖外,可以加入其他碳源,即葡萄糖,果糖,蔗糖或淀粉水解产物。 加入适量的氮源,如硝酸铵或硫酸铵。 矿物盐如磷酸二氢钾,硫酸镁,硫酸钙,氯化铁,硫酸锌和硫酸铜可加入到培养基中。 可以加入到载体中的液体介质的适量为甘蔗压浆机的干重的4至6倍,对于去皮甘蔗压浆机的干燥重量为8至14倍。 介质的最佳pH在2.0-3.0之间。 发酵温度在30-40摄氏度之间。