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1. 发明申请

US20100316989A1 METHOD FOR SCREENING AN INHIBITORY AGENT OF HBV PROLIFERATION BY USING THE INTERACTION BETWEEN HBV CAPSID AND SURFACE PROTEINS BASED ON CELLULAR IMAGING 审中-公开
标题翻译：通过使用基于细胞成像的HBV CAPSID和表面蛋白之间的相互作用来筛选HBV增殖的抑制剂的方法
公开(公告)号：US20100316989A1
公开(公告)日：2010-12-16
申请号：US12443740
申请日：2008-11-21
申请人： Hyesung Jeon , Soo Jin Oh , Yeon Gyu Yu , Yun-Kyoung Kim
发明人： Hyesung Jeon , Soo Jin Oh , Yeon Gyu Yu , Yun-Kyoung Kim
IPC分类号： C12Q1/70 , C12N15/63 , C12N5/10
CPC分类号： G01N33/5035 , C07K2319/60 , C07K2319/735 , G01N33/582 , G01N2333/02
摘要： The present invention relates to a method for screening an inhibitory agent of HBV proliferation by measuring the interaction (binding strength) between capsid protein and surface protein, necessary for the proliferation of HBV, by using cellular imaging, more precisely a method for measuring changes on cellular imaging caused by the interaction between a fusion protein containing PreS domain of HBV surface protein and PH (Pleckstrin homology) domain sequence and a fusion protein containing capsid protein and fluorescence protein (GFP) interacting with the said fusion protein. The method of the present invention detecting the interaction between proteins necessary for HBV proliferation at cellular level can be effectively used for the screening of a novel inhibitory agent of HBV proliferation at cellular level.
摘要翻译：本发明涉及通过使用细胞成像测量HBV增殖所必需的衣壳蛋白和表面蛋白之间的相互作用（结合强度）来筛选HBV增殖抑制剂的方法，更准确地说，由含有HBV表面蛋白的PreS结构域和PH（Pleckstrin同源性）结构域序列的融合蛋白和含有与所述融合蛋白相互作用的荧光蛋白（GFP）的融合蛋白之间的相互作用引起的细胞成像。检测细胞水平上HBV增殖所必需的蛋白质之间相互作用的本发明的方法可以有效地用于在细胞水平上筛选HBV增殖的新型抑制剂。

2. 发明授权

US06033889A Gene sequence of Aquifex pyrophilus superoxide dismutase and protein expressed in Escherichia coli 失效
标题翻译：在大肠埃希氏菌中表达的Aquifex pyrophilus超氧化物歧化酶和蛋白质的基因序列
公开(公告)号：US06033889A
公开(公告)日：2000-03-07
申请号：US8303
申请日：1998-01-16
申请人： Ye Sun Han , Yeon Gyu Yu , Sung Hou Kim , Jae Hwan Lim , Jae Ryeon Ryu , In Geol Choi
发明人： Ye Sun Han , Yeon Gyu Yu , Sung Hou Kim , Jae Hwan Lim , Jae Ryeon Ryu , In Geol Choi
IPC分类号： C12N15/53 , A61K38/00 , C12N9/02 , C12N5/00 , C12N15/00 , C12P21/06
CPC分类号： C12N9/0089 , A61K38/00
摘要： Gene sequences of superoxide dismutase of Aquifex pyrophilus which is one of hyperthermophile microorganism and protein expressed therefrom are provided, wherein the protein is used as a necessary medicament in treatment of inflammation, disease of autoimmunization, chromosome lesion and the like, and particularly, A. pyrophilus is a hyperthermophile which can grow at the optimum temperature of 85.degree. C., and thus the superoxide dismutase of A. pyrophilus has a higher thermal stability than other organisms, resulting in further broad applications in the pharmaceutical field.
摘要翻译：提供了作为超嗜热微生物和由其表达的蛋白质之一的Aquifex pyrophilus的超氧化物歧化酶的基因序列，其中所述蛋白质用作治疗炎症，自身免疫疾病，染色体病变等的必需药物，特别是A. 天竺葵是一种能够在85℃的最佳温度下生长的嗜热菌，因此嗜酸菌的超氧化物歧化酶比其他生物具有更高的热稳定性，从而在制药领域得到进一步的广泛应用。

3. 发明授权

US06489449B2 Peptides for inhibition of HIV infection 失效
标题翻译：用于抑制HIV感染的肽
公开(公告)号：US06489449B2
公开(公告)日：2002-12-03
申请号：US09834628
申请日：2001-04-16
申请人： Yeon Gyu Yu , Key-Sun Kim , Bong-Suk Jin
发明人： Yeon Gyu Yu , Key-Sun Kim , Bong-Suk Jin
IPC分类号： C07K1600
CPC分类号： A61K38/10
摘要： The present invention relates to peptides that can inhibit the infection of HIV, and more particularly, to peptides consisting of less than 30 amino acids which can introduce a helix capping motif into a peptide derived from C-terminal helical region (its 628-646th amino acid region) of gp41, an envelope glycoprotein of HIV, as well as consisting of the symmetrical bivalent peptide through the introduction of a branched amino acid, Fmoc-Lys(Fmoc)-OH at C-terminus of its peptide, and induce a more stable helical structure thus inhibiting the infection of HIV.
摘要翻译：本发明涉及可以抑制HIV感染的肽，更具体地说，涉及由少于30个氨基酸组成的肽，其可以将衍生自C末端螺旋区的肽（其第628-646位氨基酸性区域）gp41，HIV的包膜糖蛋白，以及通过在其肽的C末端引入支链氨基酸Fmoc-Lys（Fmoc）-OH的对称二价肽组成，并诱导更多的稳定的螺旋结构从而抑制HIV的感染。

4. 发明授权

US06294341B1 Method for detecting a substance having an activity to inhibit HIV infection using immunoassay and variant protein used for said method 失效
标题翻译：用于使用所述方法使用免疫测定法和变体蛋白检测具有抑制HIV感染活性的物质的方法
公开(公告)号：US06294341B1
公开(公告)日：2001-09-25
申请号：US09272342
申请日：1999-03-19
申请人： Yeon Gyu Yu , Sung-Hou Kim , Jae-Ryeon Ryu
发明人： Yeon Gyu Yu , Sung-Hou Kim , Jae-Ryeon Ryu
IPC分类号： G01N3353
CPC分类号： G01N33/532 , G01N2333/162 , G01N2500/00 , Y10S435/974 , Y10S530/826
摘要： The present invention relates to a method for detecting a substance having an activity to inhibit HIV infection rapidly, economically and safely. The present invention uses the characteristics that if a function of transmembrane protein gp41 of HIV is inhibited, HIV infection is also inhibited, and therefore the function of gp41 depends on the interaction between two helical structures of gp41. The method of the present invention is to detect a substance to inhibit HIV infection by an immunoassay using the interaction between the variant protein Trx-N, which is prepared by binding the N-terminal helical domain of gp41 to Trx (thioredoxin) and the variant protein GST-C, which is prepared by binding the C-terminal helical domain of gp41 with GST-C (Glutathione S-transferase). This immunoassay can be used for automatic detection of the substance to inhibit the activity of gp41 can be carried out by the method. As a result, it is possible to investigate HIV infection-inhibiting activity of a number of previously known compounds or natural compounds, and it can attribute to the development of therapeutic agent of HIV infection.
摘要翻译：本发明涉及一种经济，安全地检测具有抑制HIV感染活性的物质的方法。本发明使用的特征是，如果HIV的跨膜蛋白gp41的功能被抑制，HIV感染也被抑制，因此gp41的功能取决于gp41的两个螺旋结构之间的相互作用。本发明的方法是使用通过将gp41的N-末端螺旋结构域与Trx（硫氧还蛋白）结合而制备的变体蛋白Trx-N与变体蛋白Trx-N之间的相互作用，通过免疫测定来检测抑制HIV感染的物质，蛋白GST-C，其通过将gp41的C末端螺旋结构域与GST-C（谷胱甘肽S-转移酶）结合而制备。该免疫测定可用于自动检测物质以抑制gp41的活性，可通过该方法进行。因此，可以研究一些先前已知的化合物或天然化合物的HIV感染抑制活性，并且可以归因于HIV感染治疗剂的发展。

5. 发明授权

US06444429B1 Gene coding for DNA ligase of hyperthermophilic bacteria Aquifex pyrophilus and protein expressed therefrom 失效
标题翻译：基因编码嗜热菌嗜酸菌的DNA连接酶Aquifex pyrophilus和由其表达的蛋白质
公开(公告)号：US06444429B1
公开(公告)日：2002-09-03
申请号：US09708426
申请日：2000-11-09
申请人： Ye-Sun Han , Yeon-Gyu Yu , Jae-Hwan Lim
发明人： Ye-Sun Han , Yeon-Gyu Yu , Jae-Hwan Lim
IPC分类号： C12Q168
CPC分类号： C12N9/93 , C12Q1/6876 , C12Q2521/501
摘要： The present invention relates to a gene coding for a thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic Bacterium, a protein having an amino acid sequence expressed therefrom and a probe used for detecting said ligase DNA. More particularly, the present invention is directed to a gene having a base sequence of SEQ. ID. NO: 3 and a thermostable DNA ligase having an amino acid sequence expressed therefrom (SEQ. ID. NO: 4). Since Aquifex pyrophilus DNA ligase of the present invention has 75% of the ligation activity after heating at 95 ° C. for 1 hour, the ligase has higher stability than DNA ligases from the preexisting bacteria under high temperature and thus it can be advantageously used in genetic diseases assay of medical field, etc.
摘要翻译：本发明涉及编码来自Aquifex pyrophilus的热稳定DNA连接酶的基因，超嗜热细菌，具有由其表达的氨基酸序列的蛋白质和用于检测所述连接酶DNA的探针。更具体地，本发明涉及具有SEQ ID NO：1的碱基序列的基因。 ID。 NO.3和具有由其表达的氨基酸序列的热稳定DNA连接酶（SEQ ID NO：4）。由于本发明的Aquifex pyrophilus DNA连接酶在95℃加热后具有75％的连接活性，为1 小时，连接酶在高温下比来自预先存在的细菌的DNA连接酶具有更高的稳定性，因此可有利地用于医疗领域的遗传疾病测定等。

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