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    • 8. 发明申请
    • PLANT ACYL ACP THIOESTERASE SEQUENCES
    • 植物ACYL ACP硫酯酶序列
    • WO9513390A3
    • 1995-07-06
    • PCT/US9413131
    • 1994-11-10
    • CALGENE INCVOELKER TONI ALOISYUAN LINGKRIDL JEANHAWKINS DEBORAHJONES AUBREY
    • VOELKER TONI ALOISYUAN LINGKRIDL JEANHAWKINS DEBORAHJONES AUBREY
    • A01H5/00A23D9/00C11C3/00C12N5/10C12N9/16C12N15/09C12N15/55C12N15/82A01H5/10C11B1/00C12N15/53
    • A23D9/00C12N9/16C12N15/8247
    • This invention relates to plant thioesterases, specifically plant acyl-ACP thioesterases having substantial activity on palmitoyl-ACP substrates. DNA constructs useful for the expression of a plant palmitoyl-ACP thioesterase in a plant seed cell are described. Such constructs will contain a DNA sequence encoding the plant palmitoyl-ACP thioesterase of interest under the control of regulatory elements capable of preferentially directing the expression of the plant palmitoyl-ACP thioesterase in seed tissue, as compared with other plant tissues, when such a construct is expressed in a transgenic plant. This invention also relates to methods of using a DNA sequence encoding a plant palmitoyl-ACP thioesterase for the modification of the proportion of free fatty acids produced in a plant seed cell. Plant palmitoyl-ACP thioesterase sequences exemplified herein include Cuphea, leek, mango and elm. Transgenic plants having increased levels of C16:0 fatty acids in their seeds as the result of expression of these palmitoyl-ACP thioesterase sequences are also provided.
    • 本发明涉及植物硫酯酶,特别是对棕榈酰-ACP底物具有实质活性的植物酰基-ACP硫酯酶。 描述了用于在植物种子细胞中表达植物棕榈酰-ACP硫酯酶的DNA构建体。 与其他植物组织相比,这种构建体将含有编码感兴趣的植物棕榈酰-ACP硫酯酶的DNA序列,该植物棕榈酰-ACP硫酯酶在能够优先指导植物棕榈酰-ACP硫酯酶在种子组织中表达的调控元件的控制下, 在转基因植物中表达。 本发明还涉及使用编码植物棕榈酰-ACP硫酯酶的DNA序列来修饰植物种子细胞中产生的游离脂肪酸比例的方法。 本文例举的植物棕榈酰-ACP硫酯酶序列包括Cuphea,韭葱,芒果和榆树。 还提供了由于表达这些棕榈酰-ACP硫酯酶序列而在其种子中具有增加的C16:O脂肪酸水平的转基因植物。