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1. 发明申请

WO1996017932A1 METHOD OF INHIBITING NON SPECIFIC HYBRIDIZATION IN PRIMER EXTENSION 审中-公开
标题翻译：抑制扩展中非特异性杂交的方法
公开(公告)号：WO1996017932A1
公开(公告)日：1996-06-13
申请号：PCT/JP1995002535
申请日：1995-12-11
申请人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA , YAMANE, Akio
发明人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA
IPC分类号： C12N15/10
CPC分类号： C12Q1/6865 , C12N15/10 , C12Q1/686 , C12Q1/6869 , C12Q2525/197 , C12Q2525/117
摘要： A method of inhibiting nonspecific hybridization of a primer in the reaction of primer extension for forming a nucleic acid strand complementary to the template strand of a nucleic acid with the use of a primer, characterized by conducting the reaction of the primer with the template strand in the presence of a nucleic acid or a derivative thereof that is complementary to the primer and that has an affinity for the primer which is at most equal to the affinity of the primer for the template strand of the nucleic acid.
摘要翻译：在引物延伸的反应中抑制引物的非特异性杂交以形成与核酸的模板链互补的核酸链的引物的方法，其特征在于使引物与模板链反应存在与引物互补且对引物具有最多等于引物对核酸的模板链的亲和力的亲和力的核酸或其衍生物。

2. 发明申请

WO1995002068A1 METHOD OF DISCRIMINATING NUCLEIC ACID AND TESTING SET FOR DISCRIMINATING NUCLEIC ACID 审中-公开
标题翻译：分离核酸的方法和用于分离核酸的测试仪
公开(公告)号：WO1995002068A1
公开(公告)日：1995-01-19
申请号：PCT/JP1994001106
申请日：1994-07-07
申请人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA , OKA, Takanori , MATSUNAGA, Hironari , YAMANE, Akio
发明人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6827 , C12Q2565/102 , C12Q2545/101 , C12Q2531/113
摘要： A method of discriminating nucleic acids by examining the presence or absence of variant genes in a nucleic acid, the ratio of normal genes to variant genes, or the identity among specified genes present in various samples, which method comprises amplifying a specified region of a target nucleic acid present in a specimen by the polymerase chain reaction using a primer bearing a detectable marker and a primer bearing a site capable of binding to a solid-phase carrier, conducting competitive hybridization by adding the resultant nucleic acid as a marker sample DNA in at least an equimolar amount to a non-marker standard DNA of which the identity with the sample DNA is to be discriminated, and then measuring the marker intensity of the product of hybridization. A testing set for discriminating nucleic acids according to the above method comprises a primer to be used for amplifying a target nucleic acid and comprising a primer bearing a detectable marker and a primer bearing a site capable of binding to a solid-phase carrier, and a non-marker standard DNA of which the identity with a gene amplified by the amplifying primer is to be discriminated.
摘要翻译：通过检查核酸中变体基因的存在或不存在，正常基因与变体基因的比例或存在于各种样品中的特定基因之间的同一性来区分核酸的方法包括扩增靶的特定区域通过使用具有可检测标记的引物通过聚合酶链式反应存在于样品中的核酸和具有能够结合固相载体的位点的引物，通过将所得到的核酸作为标记物样品DNA加入进行竞争性杂交至少与待标识样品DNA的同一性的非标记标准DNA等摩尔量，然后测量杂交产物的标记强度。根据上述方法的用于鉴别核酸的测试组包括用于扩增靶核酸的引物，并且包含具有可检测标记的引物和具有能够结合固相载体的位点的引物，以及对与扩增引物扩增的基因同一性的非标记标准DNA进行鉴别。

3. 发明申请

WO1994020613A1 DETECTION OF MALARIA 审中-公开
标题翻译：检测疟疾
公开(公告)号：WO1994020613A1
公开(公告)日：1994-09-15
申请号：PCT/JP1994000324
申请日：1994-02-28
申请人： WAKUNAGA PHARMACEUTICAL CO., LTD. , WATAYA, Yusuke , YAMANE, Akio
发明人： WAKUNAGA PHARMACEUTICAL CO., LTD.
IPC分类号： C12N15/11
CPC分类号： C12Q1/6893 , C12Q2600/156 , Y02A50/58
摘要： A method of detecting tropical fever, tertian malaria, quartan malaria and Plasmodium ovale by using primers represented by formulae (1), (3) through (10). This method allows all of the plasmodia that infect the human being to be detected readily and rapidly at a high sensitivity and differentiated accurately, thus enabling malaria treatment or large-scale mass examination in the area where malaria is prevalent.
摘要翻译：通过使用由式（1），（3）至（10）表示的引物检测热带热，疟疾，四分体疟疾和卵巢疟原虫的方法。该方法允许以高灵敏度容易且快速地检测感染人的所有疟原虫，并且精确分化，从而能够在疟疾流行地区进行疟疾治疗或大规模的群体检查。

4. 发明申请

WO1989006285A1 METHOD FOR DETECTING TARGET NUCLEIC ACID IN SPECIMEN 审中-公开
标题翻译：用于检测样品中目标核酸的方法
公开(公告)号：WO1989006285A1
公开(公告)日：1989-07-13
申请号：PCT/JP1988001316
申请日：1988-12-23
申请人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA , YAMANE, Akio , OKA, Takanori , NAKAGAMI, Satoru , MIYOSHI, Kenichi
发明人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6846 , C12Q1/6832 , C12Q2563/131
摘要： A method for detecting a target nucleic acid in a specimen, which comprises hybridizing in a specimen a primer of at least one strand of the nucleic acid chains of a target nucleic acid and adding a unit nucleic acid thereto for elongation of the chain to thereby prepare a synthetic nucleic acid complementary to and hybridized with said strand, thus obtaining a copy of the target nucleic acid comprising (a) the double-stranded nucleic acid thus formed, (b) a synthetic nucleic acid prepared by separating the double-stranded nucleic acid into a single-stranded nucleic acid, or (c) a double-stranded nucleic acid which is a double-stranded hybridized product complementary to the synthetic nucleic acid, and examining whether this copy exists or not in the sample.

5. 发明申请

WO1996023077A1 PRIMER FOR GENE AMPLIFICATION, METHOD FOR NUCLEIC ACID DISCRIMINATION WITH THE USE OF THE SAME, AND NUCLEIC ACID DISCRIMINATION KIT 审中-公开
标题翻译：用于基因扩增的引物，使用其的核酸分解方法和核酸鉴定试剂盒
公开(公告)号：WO1996023077A1
公开(公告)日：1996-08-01
申请号：PCT/JP1996000151
申请日：1996-01-26
申请人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA , OKA, Takanori , YAMANE, Akio
发明人： WAKUNAGA SEIYAKU KABUSHIKI KAISHA
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6827 , C12Q2545/114 , C12Q2537/161
摘要： A method for nucleic acid discrimination with the use of the replacement of complementary chains via competitive hybridization whereby gene mutation(s) or difference(s) among genes can be clearly discriminated even though these mutation(s) or difference(s) are located in the vicinity of the binding site of the target nucleic acid to a primer. The method comprises labeling a primer for gene amplification prepared by adding a sequence non-complementary to the target nucleic acid to the 5'-end of a primer complementary to the target nucleic acid to be amplified; amplifying one of two nucleic acids, the identity of which is to be examined, by using this labeled primer; mixing the labeled DNA thus obtained with an unlabeled DNA prepared from another nucleic acid to effect competitive hybridization; and then determining the extent of the replacement of the complementary chains between these DNAs with the use of the labeling.
摘要翻译：使用通过竞争性杂交替代互补链的核酸鉴别方法，即使这些突变或差异位于其中，也可以清楚地区分基因之间的基因突变或差异目标核酸与引物的结合位点附近。该方法包括标记用于基因扩增的引物，其通过将与靶核酸不互补的序列加入与待扩增的靶核酸互补的引物的5'-末端而制备; 通过使用该标记的引物扩增两个核酸之一，其同一性将被检查; 将由此获得的标记的DNA与由另一核酸制备的未标记的DNA混合以实现竞争性杂交; 然后使用标记确定这些DNA之间的互补链的置换程度。

6. 发明专利

JPS56144355A HOT WATER DEVICE UTILIZING SOLAR HEAT 失效
公开(公告)号：JPS56144355A
公开(公告)日：1981-11-10
申请号：JP4679980
申请日：1980-04-11
申请人： YAMANE AKIO
发明人： YAMANE AKIO
IPC分类号： F24J2/04
摘要： PURPOSE:To efficiently absorb solar energy even with stationary type of a hot water device by arranging collectors over approximately half peripheral part of the outer peripheral plate of a hot water tank formed in a trapezoidal conical shape having approx. 45 deg. at the top. CONSTITUTION:A tank unit (a) is formed of a hot water tank (a1) formed in a trapezoidal conical shape having approx. 45 deg. at the top, a chilled water tank (a2) formed in the same conical shape and a heat insulating segmented chamber (a3). The tank unit (a) is installed on a post tube 4. Approximately half peripheral part of the outer peripheral plate of the hot water tank (a1) is utilized at the collector mounting body plate 7, and extension plate 7a extended obliquely downwardly is integrally formed thereat. Upper and lower collector groups are radially arranged at the collector mounting plates 7, 7a.

7. 发明专利

JPS56146950A TOWER ARRANGEMENT FOR HOT AND COLD WATER 失效
公开(公告)号：JPS56146950A
公开(公告)日：1981-11-14
申请号：JP4914980
申请日：1980-04-16
申请人： YAMANE AKIO
发明人： YAMANE AKIO
IPC分类号： F24J2/04 , F24J2/34 , F24J2/52
摘要： PURPOSE:To enable to absorb the solar energy efficiently at all times by a construction wherein the main body of tank arrangement having a truncated cone shape with collectors over nearly semicircular portion on the side surface plates of the cone is provided high up at the top of a columnar tube. CONSTITUTION:The main body of tank arrangement a consists of the hot water tank a1 having the shape of a truncated cone with an apex angle of nearly 45 deg., the cold water tank a2 having the shape of an inverse truncated cone with an apex angle of nearly 45 deg. same as the apex angle of the tank a1, and an insulation compartment a3. A vertical pipe 3 for piping is provided at the center of the main body of tank arrangement a. The upper collector group 8 consisting of a large number of pipes 8a and the lower collector group 9 consisting of a large number of pipes 9a are connectingly supported at the plate part 7 of the main body for fitting the collectors. The main body of tank arrangement a is provided high up at the top of the columnar tube 4 and the height of the columnar tube 4 is selected at will. When the collector group 8 and 9 are heated by insolation, the water is sent forth from each pipe 8a to the upper part of the hot water tank 91 and, due to the convection, the temperature of the hot water in the tank 91 rises. The hot water at the upper part of the tank 91 is supplied to the desired positions by opening the valve (not shown) of a hot water take-out pipe 24.

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