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1. 发明申请

WO9837185A3 VECTORS FOR CONTROLLED GENE EXPRESSION 审中-公开
标题翻译：受控基因表达载体
公开(公告)号：WO9837185A3
公开(公告)日：1998-11-26
申请号：PCT/US9803092
申请日：1998-02-19
申请人： UNIV TEXAS , XU HONG JI , LOGOTHETIS CHRISTOPHER J , HU SHI XUE , ZHOU YUNLI
发明人： XU HONG-JI , LOGOTHETIS CHRISTOPHER J , HU SHI-XUE , ZHOU YUNLI
IPC分类号： C07K14/47 , C07K14/525 , C12N15/63 , C12N15/85 , C12N15/861 , C12N15/00 , C12N15/67
CPC分类号： C12N15/86 , C07K14/4736 , C07K14/525 , C12N15/635 , C12N15/85 , C12N2710/10343 , C12N2830/003 , C12N2830/006 , C12N2830/008 , C12N2830/15 , C12N2830/60 , C12N2830/85
摘要： Disclosed are improved broad host range expression vectors wherein gene expression is tightly regulated by tetracycline. The disclosed tetracycline-responsive vectors provide for the creation of stable cell lines, and a method of controlled gene expression where the transient elimination of gene expression is indicated. Also disclosed are tetracycline regulated adenovirus vectors which may be efficiently propagated in 293 cells, along with other tetracycline regulated viral vectors. Also disclosed are methods of using the tetracycline regulated vectors in the treatment of diseases such as cancer.

2. 发明申请

WO2004028342A2 ENDOGENOUS GRANZYME B IN HUMAN NON-HEMATOPOIETIC CELLS 审中-公开
标题翻译：人非球形细胞中的内源性GRANZYME B
公开(公告)号：WO2004028342A2
公开(公告)日：2004-04-08
申请号：PCT/US2003/029967
申请日：2003-09-24
申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM , XU, Hong-Ji , JU, Shi-Xue , MILLS, Gordon, B.
发明人： XU, Hong-Ji , JU, Shi-Xue , MILLS, Gordon, B.
IPC分类号： A61B
CPC分类号： C12N9/6467
摘要： The instant invention relates to the determination that constitutively nearly silent GrB locus in human breast carcinoma and osteosarcoma cells activated upon retinoblastoma protein (pRB)-induced growth arrest owing to the usage of an alternative promoter/transcription start site. Cloned novel cDNA from the locus adds 34 amino acid residues to the N-terminus of GrB zymogen. The alternate product has been designated as GrB-NIC. Tumor cells with accumulated endogenous GrB-NIC, whose mature form was identical to lymphocyte GrB but with a distinctive glycosylation pattern, undergoes post-growth-arrest apoptosis that occurs concurrently with pRB cleavage, and are capable of inducing rapid apoptosis of bystander pRB - tumor cells. Expression of GrB-NIC is also observed in malignant cells of other types as well as in normal non-immune cells upon cell differentiation, especially in differentiating and differentiated neural cells. GrB-NIC plays a physiological role in embryonic, and particularly in early neuronal development. The disclosure further provides compositions and methods utilizing this new GrB-NIC technology.
摘要翻译：本发明涉及由于使用替代性启动子/转录起始位点，人乳腺癌和骨肉瘤细胞中组成型几乎无声的GrB基因座在视网膜母细胞瘤蛋白（pRB）上激活的生长停滞的测定。来自该基因座的克隆的新的cDNA向GrB酶原的N末端添加34个氨基酸残基。备用产品已被指定为GrB-NIC。具有积累的内源性GrB-NIC的肿瘤细胞，其成熟形式与淋巴细胞GrB相同但具有独特的糖基化模式，经历与pRB切割同时发生的后生长停滞的凋亡，并且能够诱导旁观者pRB < >肿瘤细胞。在细胞分化，特别是分化和分化的神经细胞中，其他类型的恶性细胞以及正常的非免疫细胞也观察到GrB-NIC的表达。 GrB-NIC在胚胎中起着生理作用，特别是在早期神经元发育中。本公开进一步提供了使用该新型GrB-NIC技术的组合物和方法。

3. 发明申请

WO2004028342A3 ENDOGENOUS GRANZYME B IN HUMAN NON-HEMATOPOIETIC CELLS 审中-公开
标题翻译：人非球形细胞中的内源性GRANZYME B
公开(公告)号：WO2004028342A3
公开(公告)日：2007-12-06
申请号：PCT/US0329967
申请日：2003-09-24
申请人： UNIV TEXAS , XU HONG-JI , JU SHI-XUE , MILLS GORDON B
发明人： XU HONG-JI , JU SHI-XUE , MILLS GORDON B
IPC分类号： A01N43/04 , A61B20060101 , A61K31/70 , C07H21/04 , C12N5/00 , C12N5/02 , C12N9/64 , C12N15/00
CPC分类号： C12N9/6467
摘要： The instant invention relates to the determination that constitutively nearly silent GrB locus in human breast carcinoma and osteosarcoma cells activated upon retinoblastoma protein (pRB)-induced growth arrest owing to the usage of an alternative promoter/transcription start site. Cloned novel cDNA from the locus adds 34 amino acid residues to the N-terminus of GrB zymogen. The alternate product has been designated as GrB-NIC. Tumor cells with accumulated endogenous GrB-NIC, whose mature form was identical to lymphocyte GrB but with a distinctive glycosylation pattern, undergoes post-growth-arrest apoptosis that occurs concurrently with pRB cleavage, and are capable of inducing rapid apoptosis of bystander pRB - tumor cells. Expression of GrB-NIC is also observed in malignant cells of other types as well as in normal non-immune cells upon cell differentiation, especially in differentiating and differentiated neural cells. GrB-NIC plays a physiological role in embryonic, and particularly in early neuronal development. The disclosure further provides compositions and methods utilizing this new GrB-NIC technology.
摘要翻译：本发明涉及由于使用替代性启动子/转录起始位点，人乳腺癌和骨肉瘤细胞中组成型几乎无声的GrB基因座在视网膜母细胞瘤蛋白（pRB）上激活的生长停滞的测定。来自该基因座的克隆新的cDNA向GrB酶原的N末端添加34个氨基酸残基。备用产品已被指定为GrB-NIC。具有积累的内源性GrB-NIC的肿瘤细胞，其成熟形式与淋巴细胞GrB相同但具有独特的糖基化模式，经历与pRB切割同时发生的后生长停滞的凋亡，并且能够诱导旁观者pRB ^{- 肿瘤细胞。在细胞分化，特别是分化和分化的神经细胞中，其他类型的恶性细胞以及正常的非免疫细胞也观察到GrB-NIC的表达。 GrB-NIC在胚胎中起着生理作用，特别是在早期神经元发育中。本公开进一步提供了使用该新型GrB-NIC技术的组合物和方法。}

4. 发明申请

WO9837091A3 MODIFIED RETINOBLASTOMA TUMOR SUPPRESSOR PROTEINS 审中-公开
标题翻译：改良的雷公藤红素抑制剂蛋白质
公开(公告)号：WO9837091A3
公开(公告)日：1998-11-05
申请号：PCT/US9803041
申请日：1998-02-19
申请人： UNIV TEXAS , BAYLOR COLLEGE MEDICINE , XU HONG JI , HU SHI XUE , BENEDICT WILLIAM F , ZHOU YUNLI
发明人： XU HONG-JI , HU SHI-XUE , BENEDICT WILLIAM F , ZHOU YUNLI
IPC分类号： C12N15/09 , A61K31/711 , A61K38/00 , A61K48/00 , A61P35/00 , C07K14/47 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C07K14/00
CPC分类号： C07K14/4736 , A01K2217/05 , A61K38/00 , A61K48/00 , C12N2799/022
摘要： Disclosed are modified broad-spectrum retinoblastoma tumor suppressor proteins that have at least the same, and in most cases higher biological activity than the corresponding wild-type retinoblastoma tumor suppressor protein. Exemplary modified retinoblastoma tumor suppressor proteins have a modified N-terminal region, in particular comprising one or more deletions and/or mutations. Also disclosed are methods of making and using the modified retinoblastoma tumor suppressor proteins, particularly in circumstances where inhibition of cell growth is desired. Thus the present disclosure provides methods for treating diseases, as exemplified by, but not limited to cancer, that are characterized by abnormal cellular proliferation.

5. 发明申请

WO1994021115A1 BROAD SPECTRUM TUMOR SUPPRESSOR GENES, GENE PRODUCTS AND METHODS FOR TUMOR SUPPRESSION GENE THERAPY 审中-公开
标题翻译：广谱光谱抑制基因，基因产物和肿瘤抑制基因治疗方法
公开(公告)号：WO1994021115A1
公开(公告)日：1994-09-29
申请号：PCT/US1994003211
申请日：1994-03-24
申请人： BAYLOR COLLEGE OF MEDICINE , XU, Hong-Ji , XU, Shi-Xue , BENEDICT, William, F.
发明人： BAYLOR COLLEGE OF MEDICINE
IPC分类号： A01N01/00
CPC分类号： A61K31/70 , A61K38/00 , A61K48/00 , C07K14/4736
摘要： The present invention relates to a broad-spectrum tumor suppressor gene and the protein expressed by that gene in appropriate hostcells. The protein is a second in-frame AUG codon-initiated retinoblasoma protein of about 94 kD relative molecular mass. The present invention also relates to methods of treating a mammal having a disease or disorder characterized by abnormal cellular proliferation, such as a tumor or cancer and methods of treating abnormally proliferating cells, such as tumor or cancer cells. Treatment is accomplished by inserting a host cell compatible p94 expression vector or an effective amount of p94 protein into a cell or cells in need of treatment.
摘要翻译：本发明涉及广谱肿瘤抑制基因和由该基因在适当的宿主细胞中表达的蛋白质。该蛋白质是第二个具有约94kD相对分子量的框内AUG密码子起始的视网膜母细胞瘤蛋白。本发明还涉及治疗以异常细胞增殖为特征的疾病或病症如肿瘤或癌症的哺乳动物的治疗方法以及治疗异常增殖细胞如肿瘤或癌细胞的方法。通过将宿主细胞相容的p94 表达载体或有效量的p94 蛋白插入到需要治疗的细胞或细胞中来完成治疗。

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