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1. 发明授权

US06834122B2 Visualization and processing of multidimensional data using prefiltering and sorting criteria 失效
标题翻译：使用预过滤和排序标准可视化和处理多维数据
公开(公告)号：US06834122B2
公开(公告)日：2004-12-21
申请号：US09767595
申请日：2001-01-22
申请人： Mary M. Yang , Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Steven J. Robles , Douglas C. Youvan
发明人： Mary M. Yang , Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Steven J. Robles , Douglas C. Youvan
IPC分类号： G06K968
CPC分类号： G06K9/00127 , G06K9/0014 , G06K9/0063 , G06K9/6253 , G06K2009/00644 , G06K2009/4657 , G06T7/0012 , G06T2200/24 , G06T2207/10016 , G06T2207/10056 , G06T2207/30024 , Y10S128/922 , Y10S707/99937
摘要： Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources. User-defined criteria can also be incorporated into the sorting process by means of a graphical user interface that comprises a visualization tools including a contour plot, a sorting bar and a grouping bar, an image window, and a plot window that allow run-time interactive identification of pixels or features meeting one or more criteria, and display of their associated spectral or kinetic data. These methods are useful for extracting information from imaging data in applications ranging from biology and medicine to remote sensing.
摘要翻译：通过数字成像光谱法生成的复杂多维数据集可以通过应用软件和基于计算机的方法进行组织和分析，包括排序算法。这些算法与图像和图形数据的组合允许像素或特征根据定义的标准快速有效地分类为有意义的组。可以基于独立排序标准来执行多轮像素或特征选择。在一个实施方案中，通过光谱标准（例如，给定波长处的强度）的排序与通过时间标准（例如，给定时间的吸光度）排序相结合，以鉴定携带突变基因的重组体的微菌落，所述突变基因编码具有期望的动力学属性和底物特异性的酶。基于先前排序（“排序和锁定”分析）中应用的标准，在后续排序中分析的像素集合的限制最小化了计算和存储资源。用户定义的标准也可以通过包括可视化工具的图形用户界面结合到分类过程中，该可视化工具包括轮廓图，分类栏和分组条，图像窗口和允许运行时间的绘图窗口满足一个或多个标准的像素或特征的交互式识别，以及它们相关联的光谱或动力学数据的显示。这些方法可用于从生物学和医学到遥感等应用中从成像数据中提取信息。

2. 发明授权

US06661909B2 Calibration of fluorescence resonance energy transfer in microscopy 失效
公开(公告)号：US06661909B2
公开(公告)日：2003-12-09
申请号：US09912117
申请日：2001-07-23
申请人： Dougalas C. Youvan , Christopher M. Silva , Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Mary M. Yang
发明人： Dougalas C. Youvan , Christopher M. Silva , Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Mary M. Yang
IPC分类号： G06K900
CPC分类号： G01N21/6458 , G01N21/278 , G01N21/6428 , G01N2021/6441
摘要： Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

3. 外观设计

USD656269S1 Hair treatment applicator 有权
公开(公告)号：USD656269S1
公开(公告)日：2012-03-20
申请号：US29364995
申请日：2010-07-01
申请人： Robert David Castagno , William J. Coleman
设计人： Robert David Castagno , William J. Coleman
CPC分类号： A45D40/26 , A45D19/0008 , A45D19/02 , A45D2019/0091 , A46B9/005 , A46B9/023

4. 发明授权

US06498026B2 Variant galactose oxidase, nucleic acid encoding same, and methods of using same 失效
标题翻译：变体半乳糖氧化酶，编码相同的核酸及其使用方法
公开(公告)号：US06498026B2
公开(公告)日：2002-12-24
申请号：US09782906
申请日：2001-02-14
申请人： Simon Delagrave , Anthony M. Maffia, III , Dennis J. Murphy , Jennifer L. Rittenhouse Pruss , Edward J. Bylina , William J. Coleman
发明人： Simon Delagrave , Anthony M. Maffia, III , Dennis J. Murphy , Jennifer L. Rittenhouse Pruss , Edward J. Bylina , William J. Coleman
IPC分类号： C12N904
CPC分类号： C12N9/0006 , D21H17/32
摘要： The present invention provides mutant genes encoding variant galactose oxidase enzymes, termed vGOs; constructs and recombinant host cells incorporating the genes; the vGO polypeptides encoded by the genes; antibodies to the polypeptides; and methods of making and using all of the foregoing.
摘要翻译：本发明提供了编码变体半乳糖氧化酶的突变基因，称为vGO; 构建体和掺入该基因的重组宿主细胞; 由该基因编码的vGO多肽; 对多肽的抗体; 以及制作和使用上述所有方法。

5. 发明授权

US06456734B1 Calibration of fluorescence resonance energy transfer in microscopy 失效
公开(公告)号：US06456734B1
公开(公告)日：2002-09-24
申请号：US09092316
申请日：1998-06-05
申请人： Douglas C. Youvan , Christopher M. Silva , Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Mary M. Yang
发明人： Douglas C. Youvan , Christopher M. Silva , Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Mary M. Yang
IPC分类号： G06K900
CPC分类号： G01N21/6458 , G01N21/278 , G01N21/6428 , G01N2021/6441
摘要： Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red). The method is demonstrated on samples exhibiting FRET between genetically engineered derivatives of the Green Fluorescent Protein (GFP) bound to the surface of Ni chelating beads by histidine-tags.

6. 外观设计

USD656667S1 Hair treatment applicator 有权
标题翻译：头发处理涂抹器
公开(公告)号：USD656667S1
公开(公告)日：2012-03-27
申请号：US29391972
申请日：2011-05-16
申请人： Robert David Castagno , William J Coleman
设计人： Robert David Castagno , William J Coleman
CPC分类号： A45D19/0008 , A45D19/02 , A45D2019/0091

7. 发明申请

US20110252686A1 High Reliability Extractor Depressor for Use in Handguns 审中-公开
标题翻译：高可靠性提取器用于手枪的压缩机
公开(公告)号：US20110252686A1
公开(公告)日：2011-10-20
申请号：US12760686
申请日：2010-04-15
申请人： William J. Coleman
发明人： William J. Coleman
IPC分类号： F41A15/10
CPC分类号： F41A15/10
摘要： A high reliability extractor depressor assembly for use in handguns which significantly reduces the effect of recoil on the extractor depressor plunger assembly. These positive effects are accomplished by the following: (a) designing a new longer piece (standoff bar) of greater mass, (b) redistributing the mass from the rear of the assembly to the front, (c) designing a new shorter piece (extractor depressor plunger), (d) reversing the location of the longer piece (standoff bar) and the shorter piece (extractor depressor plunger) in the assembly, (e) increasing total mass of the assembly, and (f) changing the balance of the assembly by shifting mass to the rear of the spring.
摘要翻译：一种用于手枪的高可靠性提取器压降组件，其显着地减少了后退对提取器压降器柱塞组件的影响。这些积极的作用是通过以下方式实现的：（a）设计一个更大质量的新的较长件（支柱），（b）将组件的后部重新分配到前部，（c）设计一个新的较短件提取器压头柱塞），（d）反转组件中较长件（支柱）和较短件（提升器压头柱塞）的位置，（e）增加组件的总质量，（f）改变组件的平衡通过将质量块移动到弹簧的后部来进行组装。

8. 发明授权

US5914245A Solid phase enzyme kinetics screening in microcolonies 失效
标题翻译：微菌落中的固相酶动力学筛选
公开(公告)号：US5914245A
公开(公告)日：1999-06-22
申请号：US98202
申请日：1998-06-16
申请人： Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Christopher M. Silva , Mary M. Yang , Douglas C. Youvan
发明人： Edward J. Bylina , William J. Coleman , Michael R. Dilworth , Christopher M. Silva , Mary M. Yang , Douglas C. Youvan
IPC分类号： C12Q1/04 , C12Q1/34 , C12Q1/44 , C12Q1/00 , C12Q1/37 , C12Q1/54
CPC分类号： C12Q1/04 , C12Q1/34 , Y10S435/808 , Y10S435/968
摘要： A MicroColonyImager instrument and solid phase methods to screen cells expressing mutagenized enzymes for enhanced activity. The MicroColonyImager instrument and methods permit high throughput screening of enzyme libraries by time course analyses of single-pixels, using either absorption, fluorescence or FRET. This high throughput assay can detect small differences in enzyme rates within microcolonies grown at a nearly confluent density on an assay disk. Each microcolony is analyzed simultaneously at single-pixel resolution, requiring less than 100 ml substrate/measurement. By simultaneously assaying different substrates tagged with spectrally distinct chromogenic or fluorogenic reporters, the substrate specificity of an enzyme can be changed.
摘要翻译： MicroColonyImager仪器和固相方法筛选表达诱变酶的细胞以增强活性。 MicroColonyImager仪器和方法允许通过使用吸收，荧光或FRET的单个像素的时程分析来酶库的高通量筛选。这种高通量测定可以检测在测定盘上以几乎汇合的密度生长的微菌落内的酶速度的小差异。每个微绒毛在单像素分辨率下同时进行分析，需要少于100毫升的底物/测量。通过同时测定用光谱不同的显色或荧光报告物标记的不同底物，可以改变酶的底物特异性。

9. 发明授权

US5806263A Glass block connector strip 失效
标题翻译：玻璃块连接条
公开(公告)号：US5806263A
公开(公告)日：1998-09-15
申请号：US597391
申请日：1996-02-08
申请人： William J. Coleman
发明人： William J. Coleman
IPC分类号： E04C1/42 , E04B1/12
CPC分类号： E04C1/42
摘要： A connector strip is disclosed for assembling a building wall of glass blocks. The strip includes a longitudinal center section having a pair of oppositely disposed and longitudinally extending grooves dimensioned to receive a centrally located ridge extending peripherally around the outer edge surfaces of a molded building block to which the strip is to be mounted. U-shaped channels extend along the center groove for receiving wiring such as fiber optics for illuminating the glass block wall. Peripheral members and secondary walls serve to strengthen the connector strip, engage marginal edge surfaces of glass blocks and facilitate cement bonding between the connector strip and the adjacent blocks. A fastener member also is provided for coupling together adjacently positioned connector strips to form a rigid framework for assembling successive rows of blocks into a wall assembly.
摘要翻译：公开了用于组装玻璃砖的建筑物墙壁的连接条。该条带包括纵向中心部分，该纵向中心部分具有一对相对设置并且纵向延伸的槽，其尺寸被设计成接收围绕待安装条带的模制构件的外缘表面周向延伸的居中定位的脊。 U形通道沿着中心槽延伸，用于接收诸如用于照亮玻璃砖壁的光纤的布线。外围构件和次级壁用于加强连接器条，接合玻璃块的边缘边缘表面，并促进连接器条和相邻块之间的粘结。还提供了紧固件构件，用于将相邻定位的连接器条连接在一起以形成刚性框架，用于将连续排的块组装成壁组件。

10. 发明授权

US10844563B1 Flood wall protection system 有权
公开(公告)号：US10844563B1
公开(公告)日：2020-11-24
申请号：US16665061
申请日：2019-10-28
申请人： William J. Coleman
发明人： William J. Coleman
IPC分类号： E02B3/10 , E06B9/00
CPC分类号： E02B3/106 , E06B2009/007
摘要： A flood protection system comprises a plurality of stackable planks which mount at their ends to a building wall with a water tight seal. The wall mount includes a flat gasket portion directly affixed to the building and outwardly extending protrusions having a substantially T-shaped configuration located thereon such that a uniquely shaped gasket connected to a plank engages the substantially T-shaped outer portion of the protrusions to permit rapid stacking and disassembly of planks. The extruded aluminum planks include internal ribbing to provide added strength while eliminating the excessive weight of conventional planks. Unique anchor means may also be provided to stabilize the planks where long spans are involved.

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