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1. 发明授权

US07776613B2 Sub-diffraction image resolution and other imaging techniques 有权
$Sub-diffraction image resolution and other imaging techniques$
标题翻译：次衍射图像分辨率等成像技术
公开(公告)号：US07776613B2
公开(公告)日：2010-08-17
申请号：US11605842
申请日：2006-11-29
申请人： Xiaowei Zhuang , Wilfred Mark Bates , Michael J. Rust
发明人： Xiaowei Zhuang , Wilfred Mark Bates , Michael J. Rust
IPC分类号： G01N21/76
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6421 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58 , G02B2207/113
摘要： The present invention generally relates to sub-diffraction image resolution and other imaging techniques. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g., by determining light emitted by the entity), then a second entity may be activated and determined. The emitted light may be used to determine the positions of the first and second entities, for example, using Gaussian fitting or other mathematical techniques, and in some cases, with sub-diffraction resolution. The methods may thus be used, for example, to determine the locations of two or more entities immobilized relative to a common entity, for example, a surface, or a biological entity such as DNA or a protein. The entities may also be determined with respect to time, for example, to determine a time-varying reaction. Other aspects of the invention relate to systems for sub-diffraction image resolution, computer programs and techniques for sub-diffraction image resolution, methods for promoting sub-diffraction image resolution, methods for producing photoswitchable entities, and the like.
摘要翻译：本发明一般涉及副衍射图像分辨率和其它成像技术。在一个方面，本发明涉及确定和/或成像来自两个或多个实体的光，所述物体被隔开的距离小于入射光的衍射极限。例如，对于可见光，实体可以分开小于约1000nm或小于约300nm的距离。在一组实施例中，实体可以是可选择性地激活的，即，一个实体可以被激活以产生光，而不激活其他实体。可以激活和确定第一实体（例如，通过确定由实体发射的光），则可以激活和确定第二实体。发射的光可以用于确定第一和第二实体的位置，例如，使用高斯拟合或其他数学技术，并且在一些情况下，使用次衍射分辨率。因此，所述方法可用于例如确定相对于共同实体（例如表面）或生物实体（例如DNA或蛋白质）固定的两个或多个实体的位置。实体也可以相对于时间来确定，例如确定时变反应。本发明的其他方面涉及用于副衍射图像分辨率的系统，用于子衍射图像分辨率的计算机程序和技术，用于促进子衍射图像分辨率的方法，用于生产可照片开关实体的方法等。

2. 发明申请

US20080032414A1 Sub-diffraction image resolution and other imaging techniques 有权
$Sub-diffraction image resolution and other imaging techniques$
标题翻译：次衍射图像分辨率等成像技术
公开(公告)号：US20080032414A1
公开(公告)日：2008-02-07
申请号：US11605842
申请日：2006-11-29
申请人： Xiaowei Zhuang , Wilfred Mark Bates , Michael J. Rust
发明人： Xiaowei Zhuang , Wilfred Mark Bates , Michael J. Rust
IPC分类号： G01N21/76
CPC分类号： G01N21/6458 , G01N21/6428 , G01N2021/6421 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58 , G02B2207/113
摘要： The present invention generally relates to sub-diffraction image resolution and other imaging techniques. In one aspect, the invention is directed to determining and/or imaging light from two or more entities separated by a distance less than the diffraction limit of the incident light. For example, the entities may be separated by a distance of less than about 1000 nm, or less than about 300 nm for visible light. In one set of embodiments, the entities may be selectively activatable, i.e., one entity can be activated to produce light, without activating other entities. A first entity may be activated and determined (e.g., by determining light emitted by the entity), then a second entity may be activated and determined. The emitted light may be used to determine the positions of the first and second entities, for example, using Gaussian fitting or other mathematical techniques, and in some cases, with sub-diffraction resolution. The methods may thus be used, for example, to determine the locations of two or more entities immobilized relative to a common entity, for example, a surface, or a biological entity such as DNA or a protein. The entities may also be determined with respect to time, for example, to determine a time-varying reaction. Other aspects of the invention relate to systems for sub-diffraction image resolution, computer programs and techniques for sub-diffraction image resolution, methods for promoting sub-diffraction image resolution, methods for producing photoswitchable entities, and the like.
摘要翻译：本发明一般涉及副衍射图像分辨率和其它成像技术。在一个方面，本发明涉及确定和/或成像来自两个或多个实体的光，所述物体被隔开的距离小于入射光的衍射极限。例如，对于可见光，实体可以分开小于约1000nm或小于约300nm的距离。在一组实施例中，实体可以是可选择性地激活的，即，一个实体可以被激活以产生光，而不激活其他实体。可以激活和确定第一实体（例如，通过确定由实体发射的光），则可以激活和确定第二实体。发射的光可以用于确定第一和第二实体的位置，例如，使用高斯拟合或其他数学技术，并且在一些情况下，使用次衍射分辨率。因此，所述方法可用于例如确定相对于共同实体（例如表面）或生物实体（例如DNA或蛋白质）固定的两个或多个实体的位置。实体也可以相对于时间来确定，例如确定时变反应。本发明的其他方面涉及用于副衍射图像分辨率的系统，用于子衍射图像分辨率的计算机程序和技术，用于促进子衍射图像分辨率的方法，用于产生可照片开关实体的方法等。

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