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1. 发明申请

WO0178288A3 DIFFERENTIABLE SPECTRAL BAR CODE METHODS AND SYSTEMS 审中-公开
标题翻译：不同的光谱条码方法和系统
公开(公告)号：WO0178288A3
公开(公告)日：2002-03-28
申请号：PCT/US0111251
申请日：2001-04-06
申请人： QUANTUM DOT CORP , EMPEDOCLES STEPHEN A , TREADWAY JOSEPH A , WATSON ANDREW R
发明人： EMPEDOCLES STEPHEN A , TREADWAY JOSEPH A , WATSON ANDREW R
IPC分类号： G01J3/28 , B01L3/00 , B82B1/00 , C40B40/06 , C40B60/14 , G01J3/443 , G01N15/00 , G01N15/14 , G01N21/25 , G01N21/31 , G01N21/64 , G01N35/00 , G06F3/00 , G06F13/42 , G06K7/00 , G06K7/12 , G06K19/06 , C30B28/12 , C22B43/00 , C22C21/00 , C30B29/46 , H01J1/02 , H01L29/06
CPC分类号： G01N21/272 , B01J2219/00317 , B01J2219/00432 , B01J2219/005 , B01J2219/00574 , B01J2219/00576 , B01J2219/00578 , B01J2219/00585 , B01J2219/00596 , B01J2219/00648 , B01J2219/00657 , B01J2219/00659 , B01J2219/00677 , B01J2219/00707 , B01J2219/00722 , B01J2219/00743 , B01L3/5025 , B01L3/502715 , B01L3/502761 , B01L3/5085 , B01L3/545 , B01L2200/0668 , B01L2300/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/0454 , B01L2400/0457 , B82Y30/00 , C40B40/06 , C40B60/14 , G01N21/25 , G01N21/253 , G01N21/274 , G01N21/278 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N21/6486 , G01N21/6489 , G01N33/54373 , G01N2015/0092 , G01N2015/1472 , G01N2015/1497 , G01N2021/6441 , G01N2021/6482 , G01N2035/00158 , G01N2035/00742 , G01N2035/00752 , G01N2201/04 , G01N2201/06113 , G01N2201/12
摘要： Devices, systems, methods (as depicted in figure 1a), and compositions of matter can track and/or identify a library of elements, particularly for use with fluids, particulates, cells, and the like. Signals from one or more semiconductor nanocrystals may be combined to define spectral codes. Separation of signal wavelengths within dedicated wavelength ranges or windows facilitates differentiation of spectral codes, while calibration signals within the spectral codes can avoid ambiguity. Modeling based on prior testing can help derive libraries of acceptable codes.
摘要翻译：装置，系统，方法（如图1a所示）和物质的组成可以跟踪和/或识别元件库，特别是用于流体，微粒，细胞等。来自一个或多个半导体纳米晶体的信号可以被组合以限定光谱代码。信号波长在专用波长范围或窗口内的分离有助于光谱代码的分化，而光谱码内的校准信号可以避免歧义。基于先前测试的建模可以帮助导出可接受代码的库。

2. 发明申请

WO0183823A8 METHODS AND COMPOSITIONS FOR POLYNUCLEOTIDE ANALYSIS USING GENERIC CAPTURE SEQUENCES 审中-公开
标题翻译：使用一般捕获序列的多核苷酸分析的方法和组合
公开(公告)号：WO0183823A8
公开(公告)日：2002-02-07
申请号：PCT/US0113979
申请日：2001-04-30
申请人： QUANTUM DOT CORP , LAI JENNIFER H , PHILLIPS VINCE E , WATSON ANDREW R
发明人： LAI JENNIFER H , PHILLIPS VINCE E , WATSON ANDREW R
IPC分类号： C07H21/02 , C07H21/04 , C12M1/34 , C12P19/34 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6844 , C12Q1/6834 , C12Q2565/519 , C12Q2537/143 , C12Q2531/113
摘要： Methods, compositions and articles of manufacture for assaying a sample for an amplification product from a target polynucleotide are provided. An amplification reaction is used to produce the amplification product from the target polynucleotide so that it can be used to indirectly assay the sample for the target polynucleotide. A sample suspected of containing the target polynucleotide is contacted with first and second primers to amplify the target polynucleotide; the first primer comprises a tag sequence, the complement of which is formed on the opposite strand during amplification and is referred to as a capture sequence. That opposite strand is referred to as a second primer extension product or an amplification product, and comprises a label. A capture probe is provided that is conjugated to a substrate and can bind to the capture sequence to form an amplification product detection complex. Methods of detecting the amplification product thus produced are also provided, as are amplification product assay arrays, along with methods of forming the same. The methods are particularly useful in multiplex settings where a plurality of target polynucleotides are to be assayed. Kits comprising reagents for performing such methods are also provided.
摘要翻译：提供了用于从靶多核苷酸测定扩增产物样品的方法，组合物和制品。使用扩增反应从靶多核苷酸产生扩增产物，使其可用于间接测定靶多核苷酸的样品。将怀疑含有靶多核苷酸的样品与第一和第二引物接触以扩增靶多核苷酸; 第一引物包含标签序列，其互补体在扩增过程中在相对的链上形成并被称为捕获序列。该相对链被称为第二引物延伸产物或扩增产物，并且包含标记。提供了捕获探针，其与底物缀合并且可以结合捕获序列以形成扩增产物检测复合物。还提供了检测如此生产的扩增产物的方法，扩增产物测定阵列以及其形成方法。所述方法在多重设置中特别有用，其中将测定多个靶多核苷酸。还提供了包含用于执行这些方法的试剂的试剂盒。

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