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    • 9. 发明公开
    • DNA MOLECULE FOR EXPRESSING HARIPIN RNA, THE CONSTRUCTING METHOD AND THE USE THEREOF
    • DNA-MOLEKÜLZUR EXPRESSION VON HAARNADEL-RNA,KONSTRUKTIONSVERFAHRENDAFÜRUND VERWENDUNG DAVON
    • EP2327782A1
    • 2011-06-01
    • EP08800572.3
    • 2008-09-04
    • Wang, LeiFan, Yunliu
    • Wang, LeiFan, Yunliu
    • C12N15/11C12N15/63C12N15/85C07H19/00C07H21/02C40B40/08C40B50/06
    • C12N15/1093C12N15/10C12N15/111C12N15/66C12N2310/14C12N2330/31C12N2330/51
    • DNA molecule for expressing hairpin RNA, the constructing method and the use thereof are provided. The DNA molecule is a closed single-strand cyclic DNA molecule which is constructed by linking four fragments A, B, C and D as following oeder: A-C-B-D or A-D-B-C, wherein A is a sense strand or anti-sense strand fragment of the target double-strand DNA; B is the fragment which is reversely complementary to A; C and D are DNA fragments with stem-loop structure and they meet one of the following requirements: 1) the nucleotide sequence of fragment C or D comprises at least one sequence e; 2) the nucleotide sequence of fragment C comprises at least one sequence e, the nucleotide sequence of fragment D comprises at least one sequence f, wherein sequences e and f individually are one strand of different restriction endonuclease recognition sequences. The DNA molecule for expressing hairpin RNA and its constructing method are useful in constructing 1hRNA library.
    • 提供用于表达发夹RNA的DNA分子,其构建方法及其用途。 DNA分子是封闭的单链环状DNA分子,其通过连接四个片段A,B,C和D如下所述构建:ACBD或ADBC,其中A是靶双链的有义链或反义链片段 品牌DNA; B是与A反向互补的片段; C和D是具有茎环结构的DNA片段,它们满足以下要求之一:1)片段C或D的核苷酸序列包含至少一个序列e; 2)片段C的核苷酸序列包含至少一个序列e,片段D的核苷酸序列包含至少一个序列f,其中序列e和f分别是不同限制性内切核酸酶识别序列的一条链。 用于表达发夹RNA的DNA分子及其构建方法可用于构建1hRNA文库。