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2. 发明公开

EP0407543A1 MAMMALIAN GAP-43 COMPOSITIONS AND METHODS OF USE 失效
标题翻译：哺乳动物GAP-43组成和使用。
公开(公告)号：EP0407543A1
公开(公告)日：1991-01-16
申请号：EP90902001.0
申请日：1989-12-22
申请人： FISHMAN, Mark C. , FEDEROFF, Howard J. , ZUBER, Mauricio X. , STRITTMATTER, Stephen M. , VALENZUELA, Dario
发明人： FISHMAN, Mark C. , FEDEROFF, Howard J. , ZUBER, Mauricio X. , STRITTMATTER, Stephen M. , VALENZUELA, Dario
IPC分类号： G01N33 , A61K38 , A61K39 , A61K47 , A61P25 , C07K1 , C07K5 , C07K7 , C07K14 , C07K16 , C07K19 , C12N5 , C12N15 , C12P21 , C12R1
CPC分类号： C07K7/06 , A61K38/00 , A61K47/62 , C07K14/4702 , C07K16/18 , C07K2319/00 , C07K2319/034 , C07K2319/61 , C07K2319/75 , C12N15/85
摘要： On a produit par clonage l'ADNc de la GAP-43 (protéine de croissance axonale) de mammifères. L'invention concerne les séquences des nucléotides et les séquences correspondantes d'acides aminés de la GAP-43 humaine et de la GAP-43 murine. Ces séquences essentiellement pures peuvent être exprimées dans des procaryotes et dans des eucaryotes hôtes et servent à surveiller et à régler la croissance neuronale dans des animaux, y compris des êtres humains. Un nouveau peptide d'acheminement vers la membrane est capable d'acheminer toute protéine ou tout peptide voulu vers la membrane cellulaire de cellules neuronales ou non neuronales. Ces séquences essentiellement pures sont utiles dans des procédés thérapeutiques et diagnostiques.

3. 发明申请

WO2002090508A2 CELL ADHESION-MEDIATING PROTEINS AND POLYNUCLEOTIDES ENCODING THEM 审中-公开
标题翻译：细胞粘附介导蛋白质和编码它们的多核苷酸
公开(公告)号：WO2002090508A2
公开(公告)日：2002-11-14
申请号：PCT/US2002/014457
申请日：2002-05-07
申请人： ALPHAGENE, INC. , STARK, Karen, A. , WEAVER, Alix , HOFFMANN, Heidi, M. , KRAUSS, Raul , VALENZUELA, Dario, B. , SAINI, Kulvinder, Singh
发明人： STARK, Karen, A. , WEAVER, Alix , HOFFMANN, Heidi, M. , KRAUSS, Raul , VALENZUELA, Dario, B. , SAINI, Kulvinder, Singh
IPC分类号： C12N
CPC分类号： C07K16/3007 , A61K2039/505 , C07K14/70503 , C07K16/30 , C07K2317/24 , C07K2319/00
摘要： The present invention provides multiple polynucleotide sequences from the same novel gene, the exons comprising the polynucleotide sequences, and the proteins encoded by the polynucleotide sequences. Three splicing variant polynucleotides were isolated from prostate tissue. The polypeptides, including the splicing variants, have a region of hydrophobicity indicative of a transmembrane domain and all three extracellular and cytoplasmic domains.
摘要翻译：本发明提供来自相同新基因的多个多核苷酸序列，包含多核苷酸序列的外显子和由多核苷酸序列编码的蛋白质。从前列腺组织中分离出三个剪接变体多核苷酸。包括剪接变体的多肽具有指示跨膜结构域和所有三个胞外和胞质结构域的疏水性区域。

4. 发明申请

WO0046406A9 ARRAYS FOR INVESTIGATING PROTEIN PROTEIN INTERACTIONS 审中-公开
标题翻译：用于研究蛋白质蛋白质相互作用的阵列
公开(公告)号：WO0046406A9
公开(公告)日：2001-09-07
申请号：PCT/US0002974
申请日：2000-02-04
申请人： ALPHAGENE INC , YUAN OLIVE YI LU , VALENZUELA DARIO , RAPEIJKO PETER , HOFFMANN HEIDI , HIGGINS KARA ANN
发明人： YUAN OLIVE YI-LU , VALENZUELA DARIO , RAPEIJKO PETER , HOFFMANN HEIDI , HIGGINS KARA ANN
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1055
摘要： A method is provided for the rapid identification of protein-protein interaction networks within a cell, tissue, or whole genome. The introduction of a multi-bait approach is a distinguishing feature of the technology. In this method a pair of two-hybrid cDNA libraries, each one carrying the complement of genes from the tissue under study, are combined for an interaction screen. A large number of yeast colonies, each identifying a protein interaction pair, are picked and distributed in single wells, providing an arrayed archive of protein-protein interactions. The archive also serves as a source of plasmids to construct arrayed replicas containing DNA of the interacting plasmid pairs. Hybridization of a given cDNA to the arrayed replicas identifies the corresponding interacting clones. Protein interaction networks are constructed by iteration of the hybridization with newly identified interacting clones.
摘要翻译：提供了一种快速鉴定细胞，组织或全基因组内蛋白质 - 蛋白质相互作用网络的方法。多诱饵方法的引入是该技术的一个显着特点。在这种方法中，组合一对双杂交cDNA文库，每个文库携带来自所研究组织的基因的补体，用于相互作用屏幕。大量的酵母菌落，每个鉴定一个蛋白质相互作用对，被挑选并分布在单个孔中，提供蛋白质 - 蛋白质相互作用的排列档案。该档案还用作构建含有相互作用质粒对DNA的阵列复制品的质粒来源。给定cDNA与阵列复制品的杂交鉴定了相应的相互作用克隆。通过与新鉴定的相互作用克隆进行杂交迭代构建蛋白质相互作用网络。

5. 发明申请

WO02090508A3 CELL ADHESION-MEDIATING PROTEINS AND POLYNUCLEOTIDES ENCODING THEM 审中-公开
标题翻译：细胞粘附介导蛋白质和编码它们的多核苷酸
公开(公告)号：WO02090508A3
公开(公告)日：2003-05-15
申请号：PCT/US0214457
申请日：2002-05-07
申请人： ALPHAGENE INC , STARK KAREN A , WEAVER ALIX , HOFFMANN HEIDI M , KRAUSS RAUL , VALENZUELA DARIO B , SAINI KULVINDER SINGH
发明人： STARK KAREN A , WEAVER ALIX , HOFFMANN HEIDI M , KRAUSS RAUL , VALENZUELA DARIO B , SAINI KULVINDER SINGH
IPC分类号： C07K14/705 , C07K16/30 , C07H21/02 , C12P21/06 , C12P15/63 , C12P15/85
CPC分类号： C07K16/3007 , A61K2039/505 , C07K14/70503 , C07K16/30 , C07K2317/24 , C07K2319/00
摘要： The present invention provides multiple polynucleotide sequences from the same novel gene, the exons comprising the polynucleotide sequences, and the proteins encoded by the polynucleotide sequences. Three splicing variant polynucleotides were isolated from prostate tissue. The polypeptides, including the splicing variants, have a region of hydrophobicity indicative of a transmembrane domain and all three extracellular and cytoplasmic domains.
摘要翻译：本发明提供来自相同新基因的多个多核苷酸序列，包含多核苷酸序列的外显子和由多核苷酸序列编码的蛋白质。从前列腺组织中分离出三个剪接变体多核苷酸。包括剪接变体的多肽具有指示跨膜结构域和所有三个胞外和胞质结构域的疏水性区域。

6. 发明申请

WO1990006948A1 MAMMALIAN GAP-43 COMPOSITIONS AND METHODS OF USE 审中-公开
标题翻译： MAMMALIAN GAP-43组合物和使用方法
公开(公告)号：WO1990006948A1
公开(公告)日：1990-06-28
申请号：PCT/US1989005808
申请日：1989-12-22
申请人： FISHMAN, Mark, C. , FEDEROFF, Howard, J. , ZUBER, Mauricio, X. , STRITTMATTER, Stephen, M. , VALENZUELA, Dario
IPC分类号： C07K07/06
CPC分类号： C07K7/06 , A61K38/00 , A61K47/62 , C07K14/4702 , C07K16/18 , C07K2319/00 , C07K2319/034 , C07K2319/61 , C07K2319/75 , C12N15/85
摘要： Mammalian GAP-43 cDNA has been cloned. The nucleotide sequences and corresponding amino acid sequences for human GAP-43 as well as rat GAP-43 are disclosed. The substantially pure sequences of the present invention may be expressed in prokaryotic and eukaryotic hosts, and are of use in monitoring and regulating neuronal growth in animals including humans. A novel membrane-targeting peptide has been discovered, which is capable of directing any desired protein or peptide to the cell membrane of neuronal or non-neuronal cells. The substantially pure sequences of the present invention are of use in therapeutic and diagnostic methods.
摘要翻译：已经克隆了哺乳动物GAP-43 cDNA。公开了人GAP-43以及大鼠GAP-43的核苷酸序列和相应的氨基酸序列。本发明的基本上纯的序列可以在原核和真核宿主中表达，并且可用于监测和调节包括人在内的动物的神经元生长。已经发现了一种新的膜靶向肽，其能够将任何所需的蛋白质或肽引导到神经元或非神经元细胞的细胞膜。本发明的基本上纯的序列可用于治疗和诊断方法。

7. 发明申请

WO0046406A3 ARRAYS FOR INVESTIGATING PROTEIN PROTEIN INTERACTIONS 审中-公开
标题翻译：用于调查蛋白质蛋白质相互作用的阵列
公开(公告)号：WO0046406A3
公开(公告)日：2001-04-12
申请号：PCT/US0002974
申请日：2000-02-04
申请人： ALPHAGENE INC , YUAN OLIVE YI LU , VALENZUELA DARIO , RAPEIJKO PETER , HOFFMANN HEIDI , HIGGINS KARA ANN
发明人： YUAN OLIVE YI-LU , VALENZUELA DARIO , RAPEIJKO PETER , HOFFMANN HEIDI , HIGGINS KARA ANN
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/1055
摘要： A method is provided for the rapid identification of protein-protein interaction networks within a cell, tissue, or whole genome. The introduction of a multi-bait approach is a distinguishing feature of the technology. In this method a pair of two-hybrid cDNA libraries, each one carrying the complement of genes from the tissue under study, are combined for an interaction screen. A large number of yeast colonies, each identifying a protein interaction pair, are picked and distributed in single wells, providing an arrayed archive of protein-protein interactions. The archive also serves as a source of plasmids to construct arrayed replicas containing DNA of the interacting plasmid pairs. Hybridization of a given cDNA to the arrayed replicas identifies the corresponding interacting clones. Protein interaction networks are constructed by iteration of the hybridization with newly identified interacting clones.
摘要翻译：提供了一种用于快速鉴定细胞，组织或全基因组内的蛋白质 - 蛋白质相互作用网络的方法。引入多诱饵方法是该技术的显着特征。在该方法中，将一对双杂交cDNA文库（每个携带来自所研究的组织中的基因的互补物）组合用于相互作用屏幕。每个鉴定蛋白质相互作用对的大量酵母菌落被挑选并分布在单个孔中，提供蛋白质 - 蛋白质相互作用的排列档案。归档还用作构建含有相互作用质粒对的DNA的排列副本的质粒来源。将给定的cDNA杂交到排列的拷贝识别相应的相互作用的克隆。蛋白质相互作用网络是通过与新鉴定的相互作用克隆进行杂交的迭代来构建的。

8. 发明申请

WO1989009256A1 REGULATION OF GENE EXPRESSION 审中-公开
标题翻译：基因表达调控
公开(公告)号：WO1989009256A1
公开(公告)日：1989-10-05
申请号：PCT/GB1989000334
申请日：1989-03-31
申请人： IMPERIAL CHEMICAL INDUSTRIES PLC , PRESIDENT AND FELLOWS OF HARVARD COLLEGE , HOLLIS, Melvyn , PIOLI, David , VALENZUELA, Dario
发明人： IMPERIAL CHEMICAL INDUSTRIES PLC , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
IPC分类号： C12N01/20
CPC分类号： C12N15/67
摘要： Gene expression is regulated by binding of a heterodimer to an asymmetric protein binding site within the gene's regulatory region.

9. 发明专利

CA2006496A1 MAMMALIAN GAP-43 COMPOSITIONS AND METHODS OF USE 未知
公开(公告)号：CA2006496A1
公开(公告)日：1990-06-22
申请号：CA2006496
申请日：1989-12-22
申请人： FISHMAN MARK C , FEDEROFF HOWARD J , ZUBER MAURICIO X , STRITTMATTER STEPHEN M , VALENZUELA DARIO
发明人： FISHMAN MARK C , FEDEROFF HOWARD J , ZUBER MAURICIO X , STRITTMATTER STEPHEN M , VALENZUELA DARIO
IPC分类号： G01N33/53 , A61K38/00 , A61K39/395 , A61K47/48 , A61P25/00 , A61P25/28 , C07K1/107 , C07K1/22 , C07K5/10 , C07K7/06 , C07K7/08 , C07K14/00 , C07K14/435 , C07K14/47 , C07K14/48 , C07K14/52 , C07K16/00 , C07K16/18 , C07K19/00 , C12N5/10 , C12N15/09 , C12N15/85 , C12P21/00 , C12P21/08 , C12R1/91 , C12N15/12 , C12N1/00 , C12N5/00 , C07K5/04 , C07K7/04 , A61K38/17 , C07K16/44
摘要： Mammalian GAP-43 cDNA has been cloned. The nucleotide sequences and corresponding amino acid sequences for human GAP-43 as well as rat GAP-43 are disclosed. The substantially pure sequences of the present invention may be expressed in prokaryotic and eukaryotic hosts, and are of use in monitoring and regulating neuronal growth in animals including humans. A novel membrane-targeting peptide has been discovered, which is capable of directing any desired protein or peptide to the cell membrane of neuronal or non-neuronal cells. The substantially pure sequences of the present invention are of use in therapeutic and diagnostic methods. A104-01.WP 122189

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