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    • 1. 发明申请
    • CELL CULTURE SPINNER FLASKS
    • 细胞培养螺旋体
    • WO0121760A9
    • 2002-10-03
    • PCT/US0026122
    • 2000-09-22
    • CELL SCIENCE THERAPEUTICS INCUPTON TODD MFLICKINGER JOHN
    • UPTON TODD MFLICKINGER JOHN
    • C12M3/04C12M3/00
    • C12M23/08C12M25/14C12M27/02
    • The present invention relates to culturing devices that are compact, utilize small amounts of cell culture media to establish and maintain cell cultures, and produce a large number of cells in a short period of time when compared to other cell culturing devices and techniques. Such devices are useful in the culture of all cell types, but are particularly useful in the culture of cells that are known in the art to be difficult to culture, including cells that lose one or more of their particular attributes/characteristics (e.g., pluripotentiality), or cells that are difficult to establish cultures of (e.g. primary cells), during culturing in traditional cell culture devices.
    • 本发明涉及紧凑的培养装置,利用少量的细胞培养基来建立和维持细胞培养物,并且与其它细胞培养装置和技术相比在短时间内产生大量的细胞。 这样的装置在所有细胞类型的培养中是有用的,但是在本领域已知的难以培养的细胞的培养中特别有用,包​​括丧失一个或多个其特定属性/特征的细胞(例如,多能性 )或在传统细胞培养装置培养期间难以建立培养物(例如原代细胞)的细胞。
    • 3. 发明申请
    • LARGE SCALE CELL CULTURE VESSEL
    • 大规模细胞培养船
    • WO2008069902A3
    • 2008-08-07
    • PCT/US2007023923
    • 2007-11-14
    • CORNING INCMARTIN GREGORY RTANNER ALLISON JUPTON TODD M
    • MARTIN GREGORY RTANNER ALLISON JUPTON TODD M
    • C12M1/00
    • C12M23/34C12M23/14C12M23/24C12M29/04
    • A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of flexible cell culture compartments in combination with a spacer material maintaining air space(s). The expandable compartments of the cell culture apparatus have imparted therein gas permeable membranes in combination with air spaces that will allow the free flow of gases between the cell culture compartments and the external environment. Furthermore an interconnecting passageway is provided between each compartment and between the cell culturing vessel and the external environment. The expandable vessel promotes the growth of large numbers of adherent or suspension cells by providing the volumes of nutrient medium and gaseous exchange.
    • 公开了一种培养细胞的多层细胞培养装置。 细胞培养装置被定义为具有多个柔性细胞培养室与保持空气间隔的间隔物组合的整体结构。 细胞培养装置的可扩张隔室在其中赋予气体可渗透的膜与空气空间的组合,这将允许细胞培养室和外部环境之间的气体自由流动。 此外,在每个隔室之间和细胞培养容器与外部环境之间提供互连通道。 可膨胀的血管通过提供体积的营养培养基和气体交换来促进大量粘附或悬浮细胞的生长。