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    • 2. 发明申请
    • FLUOROPHORE-BASED OLIGONUCLEOTIDE PROBES WITH A UNIVERSAL ELEMENT
    • 基于荧光的OLIGONUCLEOTIDE探索与通用元素
    • WO2014202938A2
    • 2014-12-24
    • PCT/GB2014000240
    • 2014-06-18
    • LGC LTDUNIV SOUTHAMPTON
    • FRENCH DAVIDDEBENHAM PAULHOWARD REBECCABROWN TOMRICHARDSON JAMES
    • C12Q1/68
    • C12Q1/6832C12Q1/6818C12Q1/6848C12Q1/6876C12Q2600/156C12Q2600/16C12Q2525/161C12Q2525/313C12Q2527/107C12Q2537/125C12Q2537/143C12Q2565/1025C12Q2565/107
    • There is provided a method of detecting the presence of a target polynucleotide and/or sequence variations within the target polynucleotide using a probe system comprising two independent partner oligonucleotide components wherein the first oligonucleotide has a first and second section, wherein the first section comprises a nucleotide sequence that is labelled with at least one visually detectable label and is not capable of hybridising to the nucleotide sequence of the target polynucleotide; and wherein the second section comprises a nucleotide sequence that is capable of hybridising to a portion of the sequence of the target polynucleotide; and the second oligonucleotide has a first and second section, wherein the first section comprises a nucleotide sequence which is capable of hybridising to a nucleotide sequence of the first section of the first oligonucleotide; and the second section comprises a nucleotide sequence that is capable of hybridising to a nucleotide sequence of the target polynucleotide that is adjacent to the nucleotide sequence that the second section of the first oligonucleotide is capable of hybridising to. There are also provided alternative methods using additional oligonucleotides and probes for use in such methods.
    • 提供了使用包含两个独立配对寡核苷酸组分的探针系统来检测靶多核苷酸存在下的靶多核苷酸和/或序列变异的方法,其中第一寡核苷酸具有第一和第二部分,其中第一部分包含核苷酸 序列,其被至少一个视觉上可检测的标记标记,并且不能与靶多核苷酸的核苷酸序列杂交; 并且其中所述第二部分包含能够与所述靶多核苷酸序列的一部分杂交的核苷酸序列; 并且所述第二寡核苷酸具有第一和第二部分,其中所述第一部分包含能够与所述第一寡核苷酸的第一部分的核苷酸序列杂交的核苷酸序列; 并且第二部分包含能够与第一寡核苷酸的第二部分能够杂交的与核苷酸序列相邻的靶多核苷酸的核苷酸序列杂交的核苷酸序列。 还提供了使用另外的寡核苷酸和探针用于这些方法的替代方法。
    • 4. 发明申请
    • APPARATUS FOR DETERMINING A DIRECTION TO A SOURCE OF RADIATION
    • 用于确定辐射源的方向的装置
    • WO2010026363A3
    • 2010-11-11
    • PCT/GB2009002069
    • 2009-08-25
    • SYMETRICA LTDUNIV SOUTHAMPTONRAMSDEN DAVIDBIRD ANTONYSKINNER GERRY
    • RAMSDEN DAVIDBIRD ANTONYSKINNER GERRY
    • G01T1/29
    • G01T1/295
    • An apparatus for determining a direction to a source of radiation, e.g. a gamma-ray or neutron source at a range of 100 m or more, is described. The apparatus is rotatable, e.g. about a vertical axis, and comprises a plurality of radiation shields and radiation detectors. The radiation shields are separated from one another by gaps to form an alternating series of radiation shields and gaps around a closed path within the plane of rotation for the apparatus. The radiation detectors are arranged to detect changes in radiation passing through gaps between the radiation shields as the apparatus is rotated. In providing a rotatable series of radiation shields and gaps with radiation detectors arranged to detect radiation passing through the gaps in this way, a radiation imager is provided that may be seen as being broadly based on coded mask techniques, but which is sensitive to radiation from all azimuths in its plane of rotation.
    • 一种用于确定辐射源的方向的装置,例如, 描述了在100μm以上的范围内的γ射线或中子源。 该装置可旋转,例如。 围绕垂直轴线,并且包括多个辐射屏蔽和辐射探测器。 辐射屏蔽通过间隙彼此分离,以在装置的旋转平面内围绕闭合路径形成交替的一系列辐射屏蔽和间隙。 放射线检测器被布置成当设备旋转时检测通过辐射屏蔽之间的间隙的辐射的变化。 在提供具有辐射检测器的可旋转系列的辐射屏蔽和间隙中,辐射检测器被布置成以这种方式检测通过间隙的辐射,提供辐射成像器,其可以被视为广泛地基于编码掩模技术,但是对于来自 其旋转平面中的所有方位角。